Yale Microarray Center for Research on the Nervous System

耶鲁大学神经系统微阵列研究中心

• 批准号: 7626807
• 负责人: SHRIKANT M MANE
• 金额: $ 137.35万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7626807
• 关键词: Arts; Autopsy; Biometry; Biotechnology; Charge; Complementary DNA; Complex; Cost Sharing; Custom; DNA Microarray Chip; Data; Detection; Development; Diagnosis; Disease; Education; Ensure; Foundations; Funding; Generic Drugs; Genes; Genetic; Glass; Human; Institution; Knowledge; Laboratories; Lasers; Methods; Microarray Analysis; Microscopy; Molecular Profiling; Mus; National Institute of Diabetes and Digestive and Kidney Diseases; National Institute of Mental Health; National Institute of Neurological Disorders and Stroke; Nervous system structure; Neurobiology; Neurologic; Neurosciences; Neurosciences Research; Performance; Physiological; Principal Investigator; Process; Proteins; RNA; Rattus; Relative (related person); Research; Research Infrastructure; Research Personnel; Resources; Sampling; Sensitivity and Specificity; Services; Slide; Solutions; Spottings; Staging; Technology; Tissues; Training and Education; analytical tool; base; biological systems; brain tissue; experience; genome-wide; improved; insight; instrumentation; laser capture microdissection; mRNA Expression; medical schools; parallel computing; prevent; programs; protein function; rat genome; repository; response; success; supercomputer; web interface

DESCRIPTION (provided by applicant): To bring the most advanced microarray technologies within reach of the largest possible number of NINDS and NIMH-supported investigators approved by the Consortium, we propose to establish a Microarray Center at Yale that will build on a substantial foundation and infrastructure that begins with the very strong Yale neuroscience program and with the Keck Foundation Biotechnology Resource Laboratory. Staff from the Keck Affymetrix GeneChip, DNA Microarray, and Biostatistics Resources will provide an extremely expert and highly dedicated team that will ensure the rapid establishment of the Center and its ultimate success. The Center will 1) provide comprehensive, state-of-the-art Affymetrix, custom and generic spotted glass slide, and ABI 1700 DNA microarray analyses to as many NINDS and NIMH-supported investigators as can be supported by the requested funding and available instrumentation, 2) provide DNA microarray and biostatistical training and education, 3) provide specialized services (e.g., custom neuroscience arrays, laser capture microscopy, and small sample amplification methods) and carry out the technology research needed to further enhance the application of DNA microarray analysis to solve challenging neuroscience research problems; 4) provide biostatistical services, develop improved analytical tools for DNA microarray analysis of neurological tissues, and move computing intensive analyses onto the Yale Biomedical supercomputer; 5) develop a NINDS/NIMH Web interface for submission of DNA microarray data into the Microarray Consortium relational data warehouse. Strengths of this application include the extensive expertise, infrastructure, and instrumentation in the Keck Lab that will support the Center; the experience of the Keck Lab at providing state-of-the-art microarray, Affymetrix, biostatistical and other technologies on a service charge basis to >1,100 principal investigators annually from >350 institutions; the experience of the Keck Microarray Resource at overseeing the recent U24-supported, NIDDK Microarray Biotechnology Center; the new Yale Center for High Performance Computation in Biomedicine that will provide unique opportunities for users of the proposed Microarray Center to solve computational challenges that are amenable to a parallel computing solution; and the very strong support of the Yale School of Medicine for the Center and which includes the cost sharing needed to equip the Center with an ABI 1700 scanner.

描述（由申请人提供）： 为了让联盟批准的尽可能多的 NINDS 和 NIMH 支持的研究人员能够接触到最先进的微阵列技术，我们建议在耶鲁大学建立一个微阵列中心，该中心将建立在坚实的基础和基础设施之上，首先是非常强大的基础设施。耶鲁大学神经科学项目和凯克基金会生物技术资源实验室。来自 Keck Affymetrix 基因芯片、DNA 微阵列和生物统计资源的工作人员将提供一支极其专业和高度敬业的团队，以确保该中心的快速建立和最终成功。该中心将 1) 向尽可能多的 NINDS 和 NIMH 支持的研究人员提供全面、最先进的 Affymetrix、定制和通用斑点载玻片以及 ABI 1700 DNA 微阵列分析，只要所请求的资金和可用仪器能够支持，2) 提供 DNA 微阵列和生物统计培训和教育，3) 提供专业服务（例如定制神经科学阵列、激光捕获显微镜和小样本扩增方法）并开展技术研究需要进一步加强DNA微阵列分析的应用，以解决具有挑战性的神经科学研究问题； 4）提供生物统计服务，开发用于神经组织DNA微阵列分析的改进分析工具，并将计算密集型分析转移到耶鲁大学生物医学超级计算机上； 5) 开发一个 NINDS/NIMH Web 界面，用于将 DNA 微阵列数据提交到微阵列联盟关系数据仓库中。该应用程序的优势包括凯克实验室丰富的专业知识、基础设施和仪器，将为该中心提供支持； Keck 实验室每年向超过 350 个机构的超过 1,100 名主要研究人员提供最先进的微阵列、Affymetrix、生物统计和其他技术（按服务收费）的经验； Keck 微阵列资源在监督最近由 U24 支持的 NIDDK 微阵列生物技术中心方面的经验；新的耶鲁大学生物医学高性能计算中心将为拟议的微阵列中心的用户提供独特的机会，以解决适合并行计算解决方案的计算挑战；以及耶鲁大学医学院对该中心的大力支持，其中包括为该中心配备 ABI 1700 扫描仪所需的费用分摊。

项目成果

Temporal specification and bilaterality of human neocortical topographic gene expression.

• DOI: 10.1016/j.neuron.2013.11.018
• 发表时间: 2014-01-22
• 期刊: Neuron
• 影响因子: 16.2
• 作者: Pletikos M;Sousa AM;Sedmak G;Meyer KA;Zhu Y;Cheng F;Li M;Kawasawa YI;Sestan N
• 通讯作者: Sestan N

Spatial mapping of structural and connectional imaging data for the developing human brain with diffusion tensor imaging.

• DOI: 10.1016/j.ymeth.2014.10.025
• 发表时间: 2015-02
• 期刊: METHODS
• 影响因子: 4.8
• 作者: Ouyang, Austin;Jeon, Tina;Sunkin, Susan M.;Pletikos, Mihovil;Sedmak, Goran;Sestan, Nenad;Lein, Ed S.;Huang, Hao
• 通讯作者: Huang, Hao

Recessive LAMC3 mutations cause malformations of occipital cortical development.

• DOI: 10.1038/ng.836
• 发表时间: 2011-06
• 期刊: Nature genetics
• 影响因子: 30.8

Susceptibility loci for intracranial aneurysm in European and Japanese populations.

• DOI: 10.1038/ng.240
• 发表时间: 2008-12
• 期刊: NATURE GENETICS
• 影响因子: 30.8
• 作者: Bilguvar, Kaya;Yasuno, Katsuhito;Niemela, Mika;Ruigrok, Ynte M.;Fraunberg, Mikael von und zu;van Duijn, Cornelia M.;van den Berg, Leonard H.;Mane, Shrikant;Mason, Christopher E.;Choi, Murim;Gaal, Emilia;Bayri, Yasar;Kolb, Luis;Arlier, Zulfikar;Ravuri, Sudhakar;Ronkainen, Antti;Tajima, Atsushi;Laakso, Aki;Hata, Akira;Kasuya, Hidetoshi;Koivisto, Timo;Rinne, Jaakko;Ohman, Juha;Breteler, Monique M. B.;Wijmenga, Cisca;State, Matthew W.;Rinkel, Gabriel J. E.;Hernesniemi, Juha;Jaaskelainen, Juha E.;Palotie, Aarno;Inoue, Ituro;Lifton, Richard P.;Guenel, Murat
• 通讯作者: Guenel, Murat

Whole-exome sequencing identifies recessive WDR62 mutations in severe brain malformations.

• DOI: 10.1038/nature09327
• 发表时间: 2010-09-09
• 期刊: Nature
• 影响因子: 64.8