GeneChip Automation and MegAllele System for Microarray Analysis at Yale Univ.

耶鲁大学用于微阵列分析的 GeneChip 自动化和 MegAllele 系统。

• 批准号: 7125786
• 负责人: SHRIKANT M MANE
• 金额: $ 105.49万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7125786
• 关键词: Address; Adopted; Affect; Arts; Autistic Disorder; Automation; Award; Biological Assay; Biological Factors; Biomedical Research; Biotechnology; Cardiovascular Diseases; Computers and Advanced Instrumentation; Contracts; Coupled; Custom; DNA; Data; Databases; Development; Disease; Ensure; Epilepsy; Equipment; Error Sources; Funding; Gene Expression; Genome; Genotype; Human Resources; Institutes; Institution; Laboratories; Malignant Neoplasms; Manuals; Mental disorders; Methodology; Methods; Microarray Analysis; Nature; Nervous system structure; Neurosciences; Participant; Performance; Productivity; Reliability of Results; Reproducibility; Research; Research Infrastructure; Research Personnel; Resources; Sampling; Services; Site; Standardization; Standards of Weights and Measures; System; Technology; Time; United States National Institutes of Health; Ursidae Family; Variant; base; editorial; experience; improved; instrumentation; research study; size

DESCRIPTION (provided by applicant): Affymetrix GeneChip microarray technology is constantly evolving with regard to the development of new arrays, expression and genotyping assays, and improved instrumentation. The increase in the size and variety of arrays coupled with their exponentially increasing use has greatly exacerbated the need for more advanced and more highly automated instrumentation. Automated methods for microarray analysis are less labor-intensive, less costly on a per sample basis, more reproducible, and are much easier to standardize across different laboratories. The Keck GeneChip facility is very heavily used to analyze gene expression and to genotype DNA. Since its founding in 2003, the GeneChip facility has analyzed >4,000 samples from >115 investigators at Yale and at 22 other institutions. The demand for services has increased by >50% in each of the last three years. Additionally, the Keck GeneChip Resource is part of the newly funded Yale Microarray Center for Research on the Nervous System. As one of four laboratories responsible for providing microarray technologies to 10,000 neuroscientists from 15 institutes in the NIH Neuroscience Blueprint, this recent award will very substantially increase the demand for Keck GeneChip services and will ensure that the requested instrumentation is used to its full capacity. In this regard, the requested GeneChip Automation Platform and MegAllele System would allow the Keck Lab to meet the anticipated increase in demand for existing services, to bring custom genotyping within reach of its users, and also would improve the reliability of the resulting data by reducing operator-to-operator variability. Funding this application would enable the GeneChip Resource to bring state-of-the-art microarray technologies to bear on several important diseases including epilepsy, psychiatric disorders, autism, cardiovascular disorders, and cancer. The strengths of this proposal include a very diverse and productive investigator user base; the experience of the PI in overseeing the Keck Affymetrix Lab and its associated Yale/NIH Microarray Center; the experience of Co-PI, K. Williams, in overseeing numerous large, multi-investigator NIH contracts and Centers; the biotechnological, statistical and database expertise of the PI and other key personnel; and the extensive infrastructure and complementary support from the Keck Lab and its associated NIH-funded Centers (e.g., the Yale/NIDA Neuroproteomics Center and the new Center for High Performance Computation in Biomedicine). If given the opportunity, the PI and key personnel will do absolutely everything in their power to ensure that the requested instrumentation has a very positive and long-lasting impact on biomedical research that will extend well beyond Yale.

描述（由申请人提供）：Affymetrix GeneChip 微阵列技术在新阵列、表达和基因分型测定以及改进仪器的开发方面不断发展。阵列的尺寸和种类的增加及其使用的指数级增长极大地加剧了对更先进和更高度自动化仪器的需求。微阵列分析的自动化方法劳动密集度较低，每个样本的成本较低，可重复性更高，并且更容易在不同实验室之间实现标准化。 Keck GeneChip 设施广泛用于分析基因表达和 DNA 基因分型。自 2003 年成立以来，GeneChip 设施已分析了来自耶鲁大学和其他 22 个机构的超过 115 名研究人员的超过 4,000 个样本。过去三年，服务需求每年增长超过 50%。此外，凯克基因芯片资源是新资助的耶鲁神经系统微阵列研究中心的一部分。作为负责向 NIH 神经科学蓝图中 15 个研究所的 10,000 名神经科学家提供微阵列技术的四个实验室之一，最近的这一奖项将极大地增加对 Keck GeneChip 服务的需求，并将确保所要求的仪器得到充分利用。在这方面，所要求的 GeneChip 自动化平台和 MegAllele 系统将使 Keck 实验室能够满足现有服务需求的预期增长，使用户能够进行定制基因分型，并且还可以通过减少操作员之间的差异。资助该应用将使基因芯片资源能够将最先进的微阵列技术应用于几种重要的疾病，包括癫痫、精神疾病、自闭症、心血管疾病和癌症。该提案的优势包括非常多样化且富有成效的研究者用户群； PI 监督 Keck Affymetrix 实验室及其相关耶鲁/NIH 微阵列中心的经验；联合 PI K. Williams 在监督众多大型、多研究者 NIH 合同和中心方面的经验； PI 和其他关键人员的生物技术、统计和数据库专业知识；以及来自 Keck 实验室及其相关 NIH 资助中心（例如耶鲁/NIDA 神经蛋白质组学中心和新的生物医学高性能计算中心）的广泛基础设施和补充支持。如果有机会，PI 和关键人员将尽其所能，确保所需的仪器对生物医学研究产生非常积极和持久的影响，这种影响将远远超出耶鲁大学。