SONGBIRD NEUROGENOMICS

鸣鸟神经基因组学

• 批准号: 8364224
• 负责人: Erich D Jarvis
• 金额: $ 0.11万
• 依托单位: CARNEGIE-MELLON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-15 至 2013-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364224
• 关键词: Behavioral; Biological; Biomedical Research; Brain; Communication; Complementary DNA; Databases; Funding; Genbank; Goals; Grant; High Performance Computing; Institutes; Japan; Length; Libraries; Messenger RNA; Molecular; National Center for Research Resources; Neurobiology; Principal Investigator; Research; Research Infrastructure; Research Personnel; Resources; Songbirds; Source; United States National Institutes of Health; Universities; abstracting; cDNA Arrays; cost; knowledge base; research study; vocal learning; zebra finch

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Keywords: cDNA, annotation, songbird, microarrays Abstract: The goal of this project is to generate a general purpose cDNA database resource of the songbird brain, for use in behavioral neurobiology experiments, particularly related to vocal learning. As of December 2002, the Genbank database lists on 72 cloned non-redundant zebra finch cDNAs, most of which are not full-length; this is certainly not enough to make evolutionary comparisons, cDNA microarrays, or other types of large scale neurogenomic comparisons. To narrow this molecular gap, we have formed a consortium of investigators from Duke University in the USA and RIKEN Institute in Japan, that has the following aims: 1) clone full-length cDNAs from a normalized zebra finch brain library that represents mRNAs expressed during commonly studied vocal communication states, 2) characterize these cDNAs by sequencing and forming a comprehensive knowledge base, and 3) use the cDNAs and knowledge base to generate and characterize non-reduntant microarrays for asking biological questions.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 关键词： cDNA、注释、鸣禽、微阵列 抽象的： 该项目的目标是生成通用 cDNA 数据库 鸣禽大脑的资源，用于行为神经生物学 实验，特别是与声乐学习相关的实验。截至 2002 年 12 月， Genbank 数据库列出了 72 个克隆的非冗余斑胸草雀 cDNA， 其中大部分都不是完整的；这当然还不足以使 进化比较、cDNA 微阵列或其他类型的大规模 神经基因组比较。为了缩小这个分子间隙，我们形成了 美国杜克大学和日本理化学研究所 (RIKEN) 的研究人员联盟 日本研究所，其目标如下：1）克隆全长 来自标准化斑胸草雀大脑文库的 cDNA，代表 mRNA 在通常研究的声音交流状态下表达，2) 通过测序并形成综合的 cDNA 来表征这些 cDNA 知识库，3) 使用 cDNA 和知识库来生成和 表征用于提出生物学问题的非冗余微阵列。