Cell Culture Core

细胞培养核心

基本信息

批准号：
7659842
负责人：
PAUL E DICORLETO
金额：
$ 15.07万
依托单位：
CLEVELAND CLINIC LERNER COM-CWRU
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-04-15 至 2014-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7659842
关键词：
American Type Culture Collection Antigens Area Biological Assay Blood Blood Vessels Bone Marrow COS-7 Cell Cannulas Cattle Cell Culture Techniques Cell Line Cells Clinic Collection Core Facility Density Gradient Centrifugation Detergents Dyes E-Selectin Economics Edetic Acid Embryo Endothelial Cells Endotoxins Exclusion Fee-for-Service Plans Femur Fibroblasts Fibronectins Ficoll Flushing Foundations Growth Heating Heparin Hour Human Human Resources Hypaque Hypothalamic structure Immunofluorescence Immunologic Incubated Inflammatory Response Instruction Label Laboratories Length Limulus MCDB 107 Maintenance Marrow Massage Maternity Hospitals Medicine Methods Modification Mononuclear Morphology Mus Mycoplasma NIH 3T3 Cells Phosphate Buffer Plastics Population Powder dose form Preparation Provider Publications Quality Control Reagent Research Personnel Resource Sharing Saline Serum Solutions Suspension Culture System Techniques Teflon Testing Thoracic aorta Trypan Blue Trypsin Umbilical cord structure Umbilical vein Veins Water acetyl-LDL base cell type cytokine density embryo cell endotoxin binding protein established cell line fetal macrophage member monocyte neoplastic cell peripheral blood pressure prevent programs response scavenger receptor seal tibia tissue culture

项目摘要

The principal objective of the Cell Culture Core Laboratory is to maintain a sufficient supply of cultured cells for the various studies that are proposed in the individual projects of this Program Project application and to provide this service with the highest level of quality control. In addition, the Core will continue to function as an educational facility in training members of the Program Project laboratories in proper cell culture methods and quality control. During the previous funding period this core laboratory has met its objective with respect to providing program investigators with sufficient numbers of characterized cultured cells for their studies. The great majority of the published reports from the program project contain experiments that were dependent on the proper operation of this core laboratory. Dozens of individuals from the project laboratories have been trained in cell culture methods and the facility has been used for cell culture studies performed by individuals in the laboratories of Project Leaders when their own lab facilities were occupied. Although all Project Leaders maintain "satellite" cell culture facilities, these investigators have been fully dependent on the core laboratory for stock flasks of cells. Core laboratory personnel have established countless long-term cultures of bovine aortic endothelial cells (EC), human umbilical vein EC, mouse aortic EC, and human foreskin fibroblasts. Many established cell lines have also been provided on a regular basis. Quality control in the facility has been excellent with minimal incidents of microbiologic contamination over the years in spite of the many individuals sharing the facility. This success reflects the fact that those using the facility are required to go through the most rigorous of mammalian cell culture training. This training will continue to be provided to all Program Project scientists performing cell culture experiments.

细胞培养核心实验室的主要目标是维持足够的培养细胞供应对于该计划项目应用程序的各个项目中提出的各种研究以及为此服务提供最高水平的质量控制。此外，核心将继续充当培训计划实验室成员的教育设施以适当的细胞培养方法和质量控制。在上一期的资金期间，该核心实验室已经达到了提供计划的目标有足够数量的特征性培养细胞进行研究的研究者。绝大多数该计划项目的发布报告包含取决于适当操作的实验这个核心实验室。来自项目实验室的数十个人已经接受了细胞培养的培训方法和该设施已用于由个人在实验室中进行的细胞培养研究项目负责人当他们自己的实验室设施被占用时。尽管所有项目负责人都保持“卫星” 细胞培养设施，这些研究人员已完全依赖于核心实验室细胞。核心实验室人员已经建立了无数的牛主动脉内皮文化细胞（EC），人脐静脉EC，小鼠主动脉EC和人包皮成纤维细胞。许多建立的细胞也定期提供线路。该设施的质量控制非常出色，在微生物学污染上最小的事件对尽管有许多人共享该设施，但多年。这种成功反映了一个事实，那些使用的人需要设施进行最严格的哺乳动物细胞培养训练。这个培训将继续向所有进行细胞培养实验的计划科学家提供。