CELLULAR AND MOLECULAR CHANGES ASSOCIATED WITH REPRODUCTIVE HEALTH

与生殖健康相关的细胞和分子变化

• 批准号: 7960056
• 负责人: Jennifer Luz Barreto Estrada
• 金额: $ 10.15万
• 依托单位: UNIVERSITY OF PUERTO RICO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7960056
• 关键词: Address; Adolescent; Affective; Agonist; Amygdaloid structure; Anabolic steroids; Androgen Analogues; Androgens; Anxiety; Behavior; Behavioral; Biochemical; Biomedical Research; Brain; Brain region; Cells; Complex; Computer Retrieval of Information on Scientific Projects Database; Cultured Cells; Data; Endorphins; Event; Funding; Gonadal Steroid Hormones; Grant; Growth Factor; Growth Factor Receptors; Hormonal; In Vitro; Institution; Knowledge; Medial; Mediating; Mental disorders; Methyltestosterone; Molecular; Neuropeptides; Neurosecretory Systems; Opioid; Peptides; Physiological; Preoptic Areas; Puberty; Puerto Rico; Radioimmunoassay; Rattus; Regulation; Reproduction; Reproductive Behavior; Reproductive Health; Research; Research Personnel; Resources; Role; Source; Symptoms; System; Testosterone; United States National Institutes of Health; base; critical developmental period; depression; immunocytochemistry; in vivo; mRNA Expression; receptor; reproductive; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Sex steroids in the brain regulate reproductive behaviors and have regulatory functions beyond the influence on reproduction. For instance, natural and synthetic androgens can induce psychiatric disorders such as anxiety and depression. Knowledge that androgens can modulate specific cellular substrates such as neuropeptides and growth factors has raised relevant questions regarding the cellular mechanisms responsible for the observed behavioral changes under physiological or abused concentrations. Neuropeptides have been associated with reproductive and anxiety-related behaviors. NPY and opioids, are highly expressed in brain regions that mediate reproductive behaviors and anxiety, but also they have been shown to be under hormonal control. However, the cellular events implied in these behaviors are poorly understood and have been addressed mainly in vivo. This proposal studies the biochemical basis underlying reproductive and anxietyrelated behaviors using an in vitro system. Isolated cells from the medial preoptic area and amygdala complex, will be exposed to testosterone and the anabolic androgenic steroid (AAS), 17¿-methyltestosterone. Cultured cells will be isolated from peripubertal rats, since puberty is a critical developmental period susceptible to hormonal and behavioral changes, and for the alarming increase in adolescents misusing AAS. Immunocytochemistry and radioimmunoassay for NPY and _-endorphin will be used to quantify IR-cells and peptide levels, respectively. Also, mRNA expression for growth factor receptors will be determined. The presence of growth factors and their receptors in the brain might suggest an important role in peptide regulation and therefore, in neuroendocrine function and anxiety. Finally, neuropeptide agonist and antagonists will be centrally infused in vivo to brain regions associated with reproductive and affective behaviors. This aim will encompass a proof of concept between the in vitro and in vivo experiments. These studies will provide critical data regarding the biochemical aspects of psychiatric symptoms associated with androgen exposure.

该副本是使用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这是调查员的机构。 大脑中的性类固醇调节生殖行为并具有调节功能 超越对繁殖的影响。例如，天然和合成雄激素可以 诱导精神疾病，例如焦虑和抑郁。知道雄激素可以 调节特定的细胞底物（例如神经肽和生长因子）已提高 有关导致观察到行为的细胞机制的相关问题 身体或滥用浓度下的变化。神经肽已关联 具有生殖和动画相关的行为。 NPY和阿片类药物在 介导生殖行为和动画的大脑区域，但它们一直是 被证明是在马力控制之下。但是，这些行为中隐含的细胞事件 知之甚少，并且主要在体内被解决。该提议研究了 使用体外的生化基础复制和焦虑行为 系统。中位前区域和杏仁核络合物的分离细胞将暴露于 睾丸激素和合成代谢雄激素类固醇（AAS），17？ - 甲基睾丸激素。培养 由于青春期是关键的发育时期 容易受到荷尔蒙和行为变化，以及青少年的惊人增加 滥用AAS。用于NPY和_- Endorphin的免疫细胞化学和放射免疫测定 分别用于量化IR细胞和肽水平。另外，mRNA表达生长 将确定因子受体。生长因素及其受体在 大脑可能暗示在肽调节中起重要作用，因此在神经内分泌中 功能和动画。最后，神经肽激动剂和拮抗剂将在中央注入 与生殖和情感行为相关的大脑区域的体内。这个目标 包括体外和体内实验之间的概念证明。这些研究会 提供有关相关精神症状的生化方面的关键数据 雄激素暴露。