Sampling and Separation Core

采样和分离核心

• 批准号: 7620681
• 负责人: Jonathan V. Sweedler
• 金额: $ 24.17万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2014-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7620681
• 关键词: Area; Bioinformatics; Biological; Biological Assay; Brain; Brain region; Cell Nucleus; Cells; Chemicals; Chemistry; Complement; Ensure; Equipment; Experimental Designs; Fractionation; Goals; Heterogeneity; Image; Investigation; Link; Liquid substance; Mass Spectrum Analysis; Measurement; Measures; Methodology; Methods; Microdialysis; Nervous system structure; Neurobiology; Neurons; Neurosciences; Peptide Signal Sequences; Peptides; Pharmaceutical Preparations; Preparation; Process; Proteins; Proteomics; Protocols documentation; Sampling; Signaling Molecule; Spatial Distribution; System; Techniques; Technology; Tissues; Work; base; brain tissue; flexibility; intercellular communication; interest; research study; success

The overarching goal of the Sampling and Separation Core is to support diverse measurements of signaling peptides and proteins using a range of approaches and then provide these samples to the Protein Identification Core for analysis. The chemical, temporal and spatial diversity of cell-cell signaling molecules requires significant expertise and planning to allow successful peptidomics and proteomics measurements. To profile signaling peptides and proteins in specific brain tissues and cells, this core specializes in: (1) collecting and fractionating peptides and proteins from biological samples, (2) creating directed sampling methodologies for single cells, spatially distinct brain regions, and biological fluids, (3) optimizing sample fractionation approaches including low-volume multidimensional separations, and (4) preparing tissues for mass spectrometry imaging. In cases where protocols and techniques do not exist for to accomplish the measurements goals, the appropriate technologies are developed. This suite of technologies will be applied to profile the signaling peptides and proteins within the nervous system in the study of fundamental neuroscience as well as pathological conditions such as drug-of-abuse-induced changes. Samples range from intact brain regions to single cells, from probing the chemical content of a tissue to measuring the subset that undergoes activity-dependent release, and from acquiring information on spatial to temporal changes in the signaling peptide complement. The core offers the flexibility to individually tailor isolation and separation methodologies for signaling peptides and related proteins inherently unique to the neurobiological phenomena under investigation by each major user.

采样和分离核心的总体目标是支持各种测量 使用一系列方法分析信号肽和蛋白质，然后将这些样本提供给 用于分析的蛋白质鉴定核心。细胞间的化学、时间和空间多样性 信号分子需要丰富的专业知识和规划才能成功进行肽组学和 蛋白质组学测量。分析特定脑组织中的信号肽和蛋白质 细胞，该核心专门从事：（1）从生物中收集和分离肽和蛋白质 样本，（2）为单细胞、空间不同的大脑创建定向采样方法 区域和生物液体，(3) 优化样品分级分离方法，包括低容量 多维分离，以及（4）准备用于质谱成像的组织。在情况下 当不存在实现测量目标的协议和技术时， 开发了适当的技术。这套技术将用于分析 基础神经科学研究中神经系统内的信号肽和蛋白质 以及病理状况，例如滥用药物引起的变化。样品范围从 完整的大脑区域到单个细胞，从探测组织的化学含量到测量 经历依赖于活动的释放的子集，并从获取空间信息到 信号肽补体的时间变化。该核心提供了单独的灵活性 为信号肽和相关蛋白质定制分离和分离方法 每个主要用户正在研究的神经生物学现象都是独一无二的。