Protein Identification Core

蛋白质鉴定核心

• 批准号: 7620687
• 负责人: Jonathan V. Sweedler
• 金额: $ 41.36万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2014-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7620687
• 关键词: Address; Arts; Bioinformatics; Biological; Brain; Cells; Communities; Complement; Complex; Custom; Data; Development; Equus caballus; Fingerprint; Fourier Transform; Fourier transform ion cyclotron resonance; Goals; Heart; Image; Imaging Techniques; Investigation; Laboratories; Mass Spectrum Analysis; Measurement; Methods; Mind; Modeling; National Institute of Drug Abuse; Nervous system structure; Neurobiology; Neurons; Organism; Peptide Signal Sequences; Peptides; Post-Translational Protein Processing; Protein Analysis; Proteins; Proteomics; Reading; Research; Sample Size; Sampling; Signal Transduction; Signaling Molecule; Structure; Techniques; Tissues; Work; base; gel electrophoresis; improved; instrument; instrumentation; intercellular communication

The overall goal of the Protein Identification Core of the UIUC Neuroproteomics Center on Cell-Cell Signaling is to characterize the protein and peptide complements of a range of samples, with a special emphasis on those involved in intercellular signaling. The intracellular signaling molecules expressed in the cells and tissues under investigation present an extremely complex analytical challenge with a vast number of components, varying dramatically in size and concentration. To address these challenges, and to provide our users with a battery of state-of-the-art protein identification techniques, a range of mass spectrometric approaches are used, including differential gel electrophoresis, mass fingerprinting, tandem sequencing, accurate mass measurements, and top-down intact protein analysis. In addition, spatial localization of intercellular signaling molecules is performed using a variety of mass spectrometric imaging techniques. Our user's needs for intercellular signaling molecule identification and quantitation in the brain are quite different from what is required from proteomics measurements of unicellular organisms and homogenous tissues, particularly with regard to sample size and complexity. Thus, the advanced measurement strategies used in our Center reflect this complexity. With the combined expertise and facilities in the research groups associated with this core, a unique opportunity exists to identify and characterize intercellular signaling peptides and proteins across a surprising range of models and scales.

UIUC神经蛋白质组学中心的蛋白质鉴定核心的总体目标在细胞细胞中 信号是要表征一系列样品的蛋白质和肽补充， 特别强调参与细胞间信号的人。细胞内信号分子 在调查的细胞和组织中表达的表达非常复杂的分析 大量组件的挑战，大小和集中度变化很大。到 应对这些挑战，并为我们的用户提供一系列最先进的蛋白质 使用识别技术，一系列质谱方法，包括差异 凝胶电泳，质量指纹，串联测序，准确的质量测量和 自上而下的完整蛋白质分析。另外，细胞间信号分子的空间定位 使用多种质谱成像技术进行。我们用户对 大脑中细胞间信号分子的识别和定量与 从单细胞生物和同质组织的蛋白质组学测量中需要什么， 特别是样本量和复杂性。因此，高级测量策略 在我们的中心中使用反映了这种复杂性。研究中的专业知识和设施 与此核心相关的群体，存在一个独特的机会来识别和表征 跨模型和尺度范围内的细胞间信号肽和蛋白质。