Interspecies Streptococcal Antagonisms in Oral Biofilms

口腔生物膜中的种间链球菌拮抗作用

• 批准号: 7750387
• 负责人: Jens Kreth
• 金额: $ 24.9万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-02-16 至 2012-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7750387
• 关键词: Address; Aerobic; Affect; Atomic Force Microscopy; Biological Models; Canned Foods; Cells; Competence; DNA; Dental Plaque; Dental caries; Development; Environment; Environmental Risk Factor; Enzymes; Etiology; Exclusion; Gene Expression; Genes; Glucose; Green Fluorescent Proteins; Growth; HA coating; Hydrogen Peroxide; Hydroxyapatites; Individual; Investigation; Libraries; Metabolic; Microbial Biofilms; Modeling; Molecular Profiling; Nutrient; Outcome; Oxidases; Oxygen; Peptides; Phase; Plasmids; Production; Pyruvate Oxidase; Research; Role; Saliva; Series; Signal Transduction; Specificity; Streptococcus gordonii; Streptococcus mutans; Testing; Time; Virulence; Virulence Factors; abstracting; bacteriocin; base; cell type; design; interest; mutant; oral bacteria; oral biofilm; oral streptococci; promoter; red fluorescent protein; research study; response

ABSTRACT The role of S. mutans in the etiology of dental caries has been well investigated. S. mutans resides in the dental plaque with up to 70 other bacterial species. Recent advances in research suggest that the cariogenic potential of dental plaque may be detemiined by the combined interactions ofthe entire oral bacteria. These interactions include modulation of gene expression, co-aggregation and growth inhibition. We hypothesize that oral streptococci compete In part by influencing virulence gene expression in the antagonistic species'by direct physical Interactions and/or metabolic signaling. The proposed Aims are designed to characterize the virulence genes of S. mutans and S. sanguinis and to identify the mechanisms how oral bacteria influence the expression of virulence gene(s) of other species. We chose the interaction of S. mutans and S. sanguinis as ideal model system because of their long known mutual exclusion. To test our hypothesis that interspecies interactions influence the outcome of the competition between S. mutans and S. sanguinis, we will: ¿ Develop a dual-species biofilm model based on saliva coated hydroxyapatite for reproducible investigation of S. mutans and S. sanguinis biofilm formation ¿ Search for new genes involved in the interspecies interaction between S. mutans and S. sanguinis ¿ Determine the expression profile of S. mutans and S. sanguinis virulence genes in response to each other ¿ Further characterize the direct physical interactions between S. mutans and S. sanguinis by atomic force microscopy

抽象的 变形链球菌在龋齿病因学中的作用已得到充分研究。 变形链球菌与多达 70 种其他细菌一起存在于牙菌斑中。 研究进展表明牙菌斑的致龋潜力可能是 由整个口腔细菌的综合相互作用决定。 包括基因表达的调节、共聚集和生长抑制。 培养口腔链球菌竞争部分通过影响毒力基因 通过直接物理相互作用和/或代谢在拮抗物种中表达 所提出的目标旨在表征金黄色葡萄球菌的毒力基因。 变形链球菌和血链球菌，并确定口腔细菌影响的机制 其他物种毒力基因的表达我们选择了变形链球菌的相互作用。 和 S. sanguinis 是理想的模型系统，因为它们长期以来众所周知的互斥性。 为了检验我们的假设，即种间相互作用影响结果 S. mutans 和 S. sanguinis 之间的竞争，我们将： ¿开发基于唾液涂层羟基磷灰石的双物种生物膜模型 变形链球菌和血链球菌生物膜形成的可重复研究 ¿寻找参与变形链球菌种间相互作用的新基因 和血红沙门氏菌 ¿确定 S. mutans 和 S. sanguinis 毒力基因的表达谱 互相回应 ¿进一步表征变形链球菌和变形链球菌之间的直接物理相互作用。 原子力显微镜下的血红