CCD Camera for Cryo-EM

用于冷冻电镜的 CCD 相机

基本信息

批准号：
7585893
负责人：
Huilin Li
金额：
$ 24.4万
依托单位：
STATE UNIVERSITY NEW YORK STONY BROOK
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-04-01 至 2010-03-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): We request a 4K by 4K CCD camera designed for cryo-EM. The camera will be installed in a JEOL JEM2010 FasTEM/STEM microscope. The cryo-EM facility is dedicated to studying the 3D structures of biological macromolecules. These protein complexes play critical roles in human health and disease. The microscope is located in Brookhaven National Lab and operated by Huilin Li, who holds a joint appointment between BNL and Biochemistry and Cell Biology Department in Stony Brook University. The large format camera and its associated software will enable microscopy automation and cryo-TEM and cryo-STEM bimodal tomography, thus significantly increase the microscopy throughput and research productivity. There are three large institutes on Long Island, New York, that conduct NIH-supported research. These are the so-called Long Island Tri-Institutes: SUNY Stony Brook being in the middle, Brookhaven National Laboratory about 15 miles to the east, and Cold Spring Harbor Laboratory about 15 miles to the west. There are numerous needs for cryo-EM imaging of large biological assemblies from local researchers. However, our cryo-EM group is the only facility available on Long Island. The requested instrument will facilitate existing NIH-funded cryo-EM projects, with the likelihood of accommodating several more projects from local biomedical community. Public Health Relevance: We request a CCD camera designed for recording digital images in an electron microscope. The microscopy facility is dedicated to studying the 3D structures of biological macromolecules. These protein complexes play critical roles in human health and disease. The large format camera and its associated software will enable microscopy automation and significantly increase the microscopy throughput and research productivity.

描述（由申请人提供）：我们要求4K乘4K CCD摄像头设计为Cryo-EM设计。该相机将安装在JEOL JEEL JEM2010 FASTEM/STEM显微镜中。冷冻EM设施致力于研究生物大分子的3D结构。这些蛋白质复合物在人类健康和疾病中起关键作用。显微镜位于布鲁克黑文国家实验室，由Huilin Li经营，他在Stony Brook University举行了BNL与生物化学和细胞生物学系之间的联合任命。大格式摄像机及其相关的软件将使显微镜自动化以及冷冻-TEM和冷冻型双峰层析成像，从而显着提高显微镜吞吐量和研究生产率。纽约长岛有三个大型机构进行了NIH支持的研究。这些是所谓的长岛三义大主义者：SUNY Stony Brook位于中间，东部约15英里的布鲁克黑文国家实验室，以及西部约15英里的冷泉港实验室。对当地研究人员的大型生物组装的冷冻成像有许多需求。但是，我们的Cryo-Em集团是长岛唯一可用的设施。要求的工具将促进现有的NIH资助的冷冻EM项目，并有可能容纳当地生物医学界的几个项目。公共卫生相关性：我们要求使用用于在电子显微镜中录制数字图像的CCD摄像头。显微镜设备致力于研究生物大分子的3D结构。这些蛋白质复合物在人类健康和疾病中起关键作用。大格式摄像机及其相关的软件将使显微镜自动化，并显着提高显微镜吞吐量和研究生产力。