Zeiss LSM 710 confocal microscope

蔡司 LSM 710 共焦显微镜

• 批准号: 7595533
• 负责人: Jeremy Nance
• 金额: $ 43.8万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-14 至 2010-05-13
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7595533
• 关键词: Address; Animal Model; Caenorhabditis elegans; Cell Shape; Cells; Communities; Confocal Microscopy; Core Facility; Developmental Biology; Disease; Drosophila eye; Embryo; Embryonic Development; Epithelial Cells; Faculty; Fluorescence; Fluorescence Microscopy; Funding; Germ Lines; Grant; Head; Heart; Human Development; Image; Investigation; Laboratories; Lasers; Learning; Life; Microscope; Morphogenesis; Movement; Organ; Pattern; Process; Proliferating; Proteins; Request for Proposals; Research; Research Personnel; Resource Sharing; Resources; Role; Specific qualifier value; Stem cells; Thick; Tissues; Training; Transcriptional Regulation; United States National Institutes of Health; Work; Zebrafish; blastomere structure; cell type; experience; medical schools; public health relevance; research study

DESCRIPTION (provided by applicant): This proposal requests support for the purchase of a Zeiss LSM 710 confocal microscope, which will be a shared resource crucial to the work of five Major User laboratories at NYU School of Medicine as well as a valuable resource to outside users in the wider NYU School of Medicine community. The projects of the Major User labs, which are funded predominantly by 6 NIH R01 grants, address both how cell types are specified during embryonic development and how these different types of cells are rearranged to form functional organs. These studies include (1) identifying mechanisms of heart differentiation and morphogenesis in zebrafish embryos (Yelon); (2) investigating patterning of the Drosophila eye and the role of global transcriptional control in cell fate decisions (Treisman); (3) determining how C. elegans early embryonic cells and epithelial cells polarize (Nance); (4) learning how germ-line stem cells in C. elegans differentiate and proliferate (Hubbard); and (5) deciphering how the zebrafish vasculature becomes patterned (Torres-Vazquez). Each project relies heavily on the use of fluorescence microscopy to visualize cell shapes and movements and to examine the subcellular localization of proteins; because the embryos or tissues imaged are relatively thick, confocal microscopy is required to remove out-of-plane fluorescence. Accessory lasers that enable use of photoactivatable proteins and simultaneous imaging of GFP and mCherry are requested to enable new and powerful types of live imaging experiments. The Major User PIs are a combination of senior and junior faculty with considerable confocal microscopy experience. Together with Dr. Alice Liang, head of the Image Core Facility, they will cooperate in supporting and operating the confocal microscope. Dr. Liang will also oversee training and use of the scope by outside investigators throughout NYU School of Medicine. PUBLIC HEALTH RELEVANCE: The microscope requested in this proposal will be crucial in advancing the research of NIH-funded projects ongoing at NYU School of Medicine, and the requested accessory laser lines will enable new lines of investigation. Collectively the projects use model organisms to address fundamental questions in developmental biology, including how cells are patterned and organized to form organs. A basic understanding of these processes is critical to understanding human development and disease.

描述（由申请人提供）：该提案请求支持购买蔡司 LSM 710 共焦显微镜，这将是对纽约大学医学院五个主要用户实验室的工作至关重要的共享资源，也是对外的宝贵资源更广泛的纽约大学医学院社区的用户。主要用户实验室的项目主要由 6 项 NIH R01 拨款资助，研究胚胎发育过程中细胞类型如何指定以及这些不同类型的细胞如何重新排列以形成功能器官。这些研究包括（1）确定斑马鱼胚胎（Yelon）心脏分化和形态发生的机制； (2) 研究果蝇眼睛的模式以及全局转录控制在细胞命运决定中的作用（Treisman）； (3)确定线虫早期胚胎细胞和上皮细胞如何极化(Nance)； (4) 了解秀丽隐杆线虫中的生殖系干细胞如何分化和增殖（Hubbard）； (5) 破译斑马鱼脉管系统如何形成图案（Torres-Vazquez）。每个项目都严重依赖于使用荧光显微镜来可视化细胞形状和运动并检查蛋白质的亚细胞定位；由于成像的胚胎或组织相对较厚，因此需要共焦显微镜来去除面外荧光。需要能够使用光激活蛋白质并同时对 GFP 和 mCherry 进行成像的辅助激光器，以实现新型、强大的实时成像实验。主要用户 PI 由具有丰富共焦显微镜经验的高级和初级教师组成。他们将与图像核心设施负责人 Alice Liang 博士一起合作支持和操作共焦显微镜。梁博士还将监督整个纽约大学医学院外部研究人员的培训和使用范围。公共健康相关性：本提案中要求的显微镜对于推进纽约大学医学院正在进行的 NIH 资助项目的研究至关重要，而所需的辅助激光线将实现新的研究方向。这些项目共同利用模型生物来解决发育生物学中的基本问题，包括细胞如何形成模式和组织形成器官。对这些过程的基本了解对于理解人类发育和疾病至关重要。

项目成果

Plexin D1 negatively regulates zebrafish lymphatic development.

Plexin D1 负向调节斑马鱼淋巴管发育。

• DOI: 10.1242/dev.200560
• 发表时间: 2022
• 期刊: Development (Cambridge, England)
• 作者: Britto,DenverD;He,Jia;Misa,JuneP;Chen,Wenxuan;Kakadia,PurviM;Grimm,Lin;Herbert,CaitlinD;Crosier,KathrynE;Crosier,PhilipS;Bohlander,StefanK;Hogan,BenjaminM;Hall,ChristopherJ;Torres-Vázquez,Jesús;Astin,JonathanW
• 通讯作者: Astin,JonathanW

Anosmin1 Shuttles Fgf to Facilitate Its Diffusion, Increase Its Local Concentration, and Induce Sensory Organs.

Anosmin1 穿梭 Fgf 以促进其扩散，增加其局部浓度，并诱导感觉器官。

• DOI: 10.1016/j.devcel.2018.07.015
• 发表时间: 2018
• 期刊: Developmental cell
• 影响因子: 11.8
• 作者: Wang,John;Yin,Yandong;Lau,Stephanie;Sankaran,Jagadish;Rothenberg,Eli;Wohland,Thorsten;Meier-Schellersheim,Martin;Knaut,Holger
• 通讯作者: Knaut,Holger