Self-Cleaning Sensor Membranes to Improve Glucose Monitoring In Vivo

自清洁传感器膜可改善体内血糖监测

基本信息

批准号：
7920084
负责人：
Melissa Grunlan
金额：
$ 17.44万
依托单位：
TEXAS ENGINEERING EXPERIMENT STATION
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-07-17 至 2012-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7920084
关键词：
Affect Alexa594 Animals Behavior Binding Binding Sites Biological Assay Biosensing Techniques Biosensor Blood Glucose Cell Adhesion Cells Chemical Engineering Chemistry Chronic Disease Collaborations Concanavalin A Dependence Detection Development Devices Dextrans Diabetes Mellitus Diffusion Dimensions Disease Excision Fingers Fluorescence Forearm Glucose Goals Health Histologic Housing Hydrogels Hydrophobicity Immune response Implant In Vitro Individual Intercellular Fluid International Light Measurement Measures Mechanics Membrane Methodology Methods Monitor Non-Invasive Cancer Detection Optics Oxidation-Reduction Patients Phase Transition Photobleaching Polymers Preparation Property Protocols documentation Rattus Reading Recording of previous events Research Retrieval Series Signal Transduction Siloxanes Skin Solid Source Surface Surface Properties Swelling System Technology Temperature Testing Texas Time Tissues Transition Temperature Tube Universities Water Work World Health Organization acrylic acid base capsule chemical synthesis colloidal nanoparticle crosslink density design dextran diabetic diabetic rat experience fluorophore glucose monitor glucose sensor hydrophilicity implantation implanted sensor improved in vivo monomer multidisciplinary nanocomposite nanoparticle novel novel strategies optical sensor prevent public health relevance sensor

项目摘要

DESCRIPTION (provided by applicant): Diabetes is an international health concern with millions of patients worldwide. There is an unmet need to develop a biosensor technology that is a fast and convenient way to monitor blood glucose in diabetics. Thus, the particular goal of this research is the development of a self-cleaning hydrogel sensor membrane which undergoes cyclical, thermally driven removal of adhered cells to improve the efficacy and lifetime of an implanted glucose sensor. Implanted optically based sensors have the potential for continuous detection of an analyte (e.g. glucose). However, sensing is often compromised by the attachment and accumulation of cells from surrounding tissue as part of the host response. As a result, glucose diffusion is diminished and the sensor must be removed and replaced. We will develop novel self-cleaning thermoresponsive nanocomposite hydrogel membranes fabricated in the form of a hollow tube that houses a fluorescent glucose-responsive assay. The sensor will be implanted in the interstitial fluid just beneath the skin. After implantation, the glucose specific sensor will, when illuminated with the optical system developed, provide continuous measurement of fluorescence peaks that are proportional to the glucose concentration. There are three specific aims proposed in this research: (1) iteratively develop thermoresponsive nanocomposite hydrogel sensor membranes and characterize their mechanical properties as well as thermally-modulated swelling/dewelling behavior, surface hydrophilicity/- hydrophobicity, glucose diffusion and cell-release behavior, (2) iteratively evaluate the efficacy of selected thermoresponsive nanocomposite hydrogels sensor membranes to house a glucose-responsive assay, and (3) quantify the in vivo efficacy of the sensors prepared from selected self-cleaning hydrogel membranes containing the glucose-responsive assay, using both normal and diabetic rats. The facilities and individuals represented in this multidisciplinary team from the Biomedical and Chemical Engineering Departments at Texas A&M University are uniquely suited to carry out this research and have expertise in the chemical synthesis of thermoresponsive hydrogels, optical and electrochemical glucose biosensing, as well as biomedical and chemical engineering. The team has a history of collaboration and brings over 18 years of experience in the design and development of optical sensors for glucose and other analytes. PUBLIC HEALTH RELEVANCE: Diabetes mellitus is a debilitating, chronic, disease that affects over 180 million people, according to the World Health Organization, with estimates projecting to 366 million in 2030. The disease requires the patient to monitor glucose levels several times daily. This monitoring is currently non-continuous and performed primarily by using a commercially available finger or forearm stick method blood glucose reading device. Thus, the ultimate goal of this work is the development of an implantable self-cleaning glucose sensor that, once implanted, could be used to monitor glucose continuously with light from a watch-type of device to help patients with diabetes mellitus.

描述（由申请人提供）：糖尿病是国际健康问题，全球数百万患者。不满足的需要开发一种生物传感器技术，这是一种快速，方便的方法来监测糖尿病患者血糖的方法。因此，这项研究的特殊目标是开发自我清洁的水凝胶传感器膜，该膜经过周期性的热驱动的粘附细胞去除，以提高植入的葡萄糖传感器的功效和寿命。植入的基于光学的传感器具有连续检测分析物（例如葡萄糖）的潜力。然而，作为宿主反应的一部分，细胞的附着和积累通常会损害传感。结果，葡萄糖扩散会减小，必须卸下传感器并更换。我们将开发出新型的自我清洁热纳米复合材料水凝胶膜，该水凝胶膜以空心管的形式制造，该管子容纳荧光葡萄糖反应测定。传感器将植入皮肤下方的间隙液中。植入后，当开发的光学系统照明时，葡萄糖特异性传感器将连续测量与葡萄糖浓度成正比的荧光峰。这项研究提出了三个特定的目的：（1）迭代发展热反应性的纳米复合水凝胶传感器膜，表征了它们的机械性能以及热调节的肿胀/脱水行为，表面亲水性/ - 疏水性/ - 疏水性，葡萄糖和细胞的渗透行为（2）的渗透性，（2）的渗透性，（2）的渗透率（2）效果（2）的渗透性（2）的渗透率（2）使用正常和糖尿病大鼠，膜上容纳葡萄糖反应测定的膜，（3）通过含有葡萄糖反应性测定的选定的自我清洁水凝胶膜制备的传感器的体内功效。德克萨斯A＆M大学生物医学和化学工程系的该多学科团队中代表的设施和个人非常适合进行这项研究，并且在热培养基水凝胶，光学和电化学葡萄糖生物化的化学合成方面具有专业知识。该团队拥有合作的历史，并在葡萄糖和其他分析物的光学传感器设计和开发方面具有18年的经验。公共卫生相关性：根据世界卫生组织的数据，糖尿病是一种令人衰弱的慢性疾病，影响超过1.8亿人，估计在2030年预计将为3.66亿。该疾病要求患者每天监测几次葡萄糖水平。该监测当前是非连续的，主要通过使用市售的手指或前臂棒方法血糖阅读装置进行。因此，这项工作的最终目标是开发可植入的自我清洁葡萄糖传感器，该传感器一旦植入，就可以使用手表类型的设备手表的光线连续监测葡萄糖，以帮助糖尿病患者。

项目成果

期刊论文数量（4）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Thermoresponsive Nanocomposite Hydrogels: Transparency, Rapid Deswelling and Cell Release.

热响应纳米复合水凝胶：透明、快速消溶胀和细胞释放。

DOI：
10.1166/jbt.2011.1005
发表时间：
2011
期刊：
Journal of biomaterials and tissue engineering
影响因子：
0.1
作者：
Hou,Yaping;Fei,Ruochong;Burkes,JonathanC;Lee,ShinDuk;Munoz-Pinto,Dany;Hahn,MariahS;Grunlan,MelissaA
通讯作者：
Grunlan,MelissaA

Thermoresponsive nanocomposite double network hydrogels.