Core Rederivation and Barrier Renovation

核心重新衍生和屏障改造

• 批准号: 7935559
• 负责人: James W Tracy
• 金额: $ 642.85万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-04 至 2013-03-03
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7935559
• 关键词: Aging; Animal Barrier Facility; Breeding; Cryopreservation; Embryo; Embryo Transfer; Equipment; Event; Film; Floor; Gnotobiotic; Housing; Kentucky; Laboratories; Location; Movement; Organism; Research; Research Infrastructure; Research Personnel; Resources; Rodent; Security; Services; Sterility; Sterilization; Transgenic Organisms; Universities; aged; animal facility; flexibility; microbial; sperm cell; square foot

DESCRIPTION (provided by applicant): The University of Kentucky (UK) requests $6,432,134 to renovate 5,526 sq. ft. on the fourth floor of the Sanders-Brown Center for Aging. The proposed renovation will completely demolish and remove the present aged and obsolete small barrier animal facility. The new facility will provide three critically needed specific core capabilities that are presently not available at UK. A centralized core embryo cryopreservation and embryo transfer laboratory will be constructed and outfitted. This laboratory will provide embryo transfer rederivation of both in-house stock and imported transgenic strains to eliminate pathogenic or commensal organisms potentially altering research results. Additionally this laboratory will provide both sperm and embryo cryopreservation services to UK investigators permitting the long- term storage of unique transgenic strains as an alternative to continual breeding or as a research resource in the event of unanticipated colony loss. This laboratory will be adjacent to a small high- security sterile barrier for initial expansion of rederived strains prior to movement to conventional animal facilities. Finally, a room for gnotobiotic rodent housing will be constructed and outfitted with three flexible film isolators to support the burgeoning need for transgenic strains with defined microbial flora. By locating these three facilities in this single location we will maximally leverage the available support sterilization equipment to provide a set of core services that have been identified by the investigators as a critical infrastructure need.

描述（由申请人提供）：肯塔基大学（英国）请求 6,432,134 美元来翻修桑德斯-布朗老龄化中心四楼 5,526 平方英尺的空间。本次改造将彻底拆除现有老化、陈旧的小型栅栏动物设施。新设施将提供英国目前尚不具备的三项急需的特定核心能力。将建造并配备一个集中的核心胚胎冷冻保存和胚胎移植实验室。该实验室将为内部库存和进口转基因菌株提供胚胎移植再衍生，以消除可能改变研究结果的致病或共生生物。此外，该实验室还将向英国研究人员提供精子和胚胎冷冻保存服务，允许长期储存独特的转基因品系，作为持续育种的替代方案，或在出现意外的菌落损失时作为研究资源。该实验室将毗邻一个小型高安全性无菌屏障，用于在转移到传统动物设施之前对再衍生菌株进行初步扩增。最后，将建造一个用于限生啮齿动物住房的房间，并配备三个灵活的薄膜隔离器，以满足对具有明确微生物菌群的转基因菌株日益增长的需求。通过将这三个设施安置在这个单一地点，我们将最大限度地利用可用的支持灭菌设备来提供一组核心服务，这些服务已被调查人员确定为关键基础设施需求。