Core A: Animal Models Core

核心 A：动物模型核心

基本信息

批准号：
10447739
负责人：
Ryan B Sartor
金额：
$ 24.97万
依托单位：
UNIV OF NORTH CAROLINA CHAPEL HILL
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-09-19 至 2024-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10447739
关键词：
Animal Model Animals Bacteria Breeding Cell Communication Cell Lineage Cells Chronic Complex Country Crossbreeding Derivation procedure Development Ecology Environment Epithelial Epithelial Cells Faculty Funding Genes Genetic Genetic Transcription Germ-Free Gnotobiotic Goals Homeostasis Housing Human Human Resources Immune Immune response Immunocompromised Host Individual Intestinal Mucosa Intestines Laboratories Leadership Maintenance Mammals Measures Mediating Microbe Modeling Mouse Strains Mucous Membrane Mus Mutant Strains Mice Phenotype Physiological Proteins Relapse Reporter Research Personnel Resolution Resources Rodent Role Services Stretching Tissues Transgenic Mice Transgenic Organisms Universities Variant Washington Work Zebrafish animal facility animal resource cell type cost cost effective experience experimental study fecal transplantation germ free condition gut inflammation gut microbiota improved in vivo imaging innovation intestinal barrier intestinal homeostasis microbial microbial host microbiota mouse model mutant novel prevent programs response success wasting

项目摘要

The Animal Models Core centralizes breeding, development and maintenance of extremely useful, but complex mouse and zebrafish models by providing a stable environment for each centrally housed line to increase efficiency, decrease costs and prevent phenotype variation. This Core leverages exceptional specific pathogen free (SPF) and gnotobiotic mice and zebrafish facilities and expert personnel to provide high-quality resources and services to all 3 current and proposed Projects. Core A personnel work closely with Project investigators to provide SPF and gnotobiotic mouse and zebrafish models that are essential to accomplish goals of each of the current projects. This core provides prioritized creation of novel SPF mouse strains and derivation of germ-free (GF) lines that will support the success of Projects 1, 2, 3 and 4. In addition, this Core has created highly innovative zebrafish models to facilitate activities in Projects 3 and 4. By providing technical experience and central housing to create and maintain GF wild type, mutant and double mutant mouse strains, Core A allows investigators to efficiently perform innovative, mechanistic experiments far beyond the resources of traditional R01-supported investigators and extends the capabilities of our national gnotobiotic resource. These GF mice and zebrafish allows Project investigators to manipulate the complex gut microbial ecology to study the mechanistic role of luminal microbes on immune and epithelial responses in gnotobiotic animals colonized with defined microbiota. The Specific Aims of the Animal Models Core are to provide P01 Projects with: 1.Centralized breeding of specific pathogen free and germ-free mice and zebrafish to eliminate redundancy in breeding units, diminish expenses, decrease environmental variation by maintaining central housing facilities and minimize waste by promoting shared utilization of animals by multiple investigators; 2.Create new mouse and zebrafish models including importing existing mutant strains for central breeding, creating new strains, including cell-specific mutants by crossbreeding of existing mutant, transgenic and fluorescent protein reporter lines, and generating new transgenic and mutant lines; 3.Germ-free derivation of existing and new mouse strains that can be used to evaluate the impact of complex resident microbiota individual microbial species or microbial consortia on intestinal inflammation and mucosal homeostasis. This Core supports Projects 1, 2, 3 and 4 in the renewal application.

动物模型核心将非常有用的繁殖，开发和维护集中通过为每条中央线的稳定环境提供稳定的环境，提高效率，降低成本并防止表型变化。该核心利用特定的特定特定无病原体（SPF）和gnotobiotic小鼠和斑马鱼设施以及专业人员提供高质量所有3个当前和拟议项目的资源和服务。核心A人员与项目调查人员紧密合作，以提供SPF和GNOTOBIORIC MEOX和斑马鱼模型对于实现当前每个项目的目标至关重要。此核心提供优先创建新型SPF小鼠菌株和无菌（GF）线的衍生物，将支持该系项目1、2、3和4的成功。此外，此核心为高度创新的斑马鱼模型创造了通过提供技术经验和中央住房来创建和维持GF野生型，突变体和双突变小鼠菌株，核心A允许研究人员有效进行创新的机械实验远远超出了传统R01支持的资源调查人员并扩展了我们国家gnotobiotic资源的能力。这些GF小鼠和斑马鱼允许项目研究人员操纵复杂的肠道微生物生态学，以研究腔微生物对gnotobirotic动物的免疫和上皮反应，用定义的微生物群定殖。动物模型核心的具体目的是为P01项目提供： 1.特定的无病原体和无细菌小鼠和斑马鱼的中央化育种以消除繁殖单位的冗余，减少费用，通过维持中心减少环境变化住房设施并通过促进多个研究人员对动物的共同利用来最大程度地减少废物； 2.创建新的小鼠和斑马鱼模型，包括进口现有的突变菌株以用于中央育种，创建新菌株，包括通过现有突变体，转基因和荧光蛋白报道系，并产生新的转基因和突变系； 3.现有和新的老鼠菌株的无元素推导，可用于评估的影响复杂的居民微生物群体单个微生物或微生物财团在肠道炎症和粘膜稳态。该核心支持续订应用程序中的项目1、2、3和4。