Functional Microbiomics Core

功能微生物组学核心

• 批准号: 10492098
• 负责人: HARIBABU BODDULURI
• 金额: $ 75.81万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-03-01 至 2028-02-29
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10492098
• 关键词: 3xTg-AD mouse; Administrative Supplement; Adopted; Aging; Alzheimer' s Disease; Area; Award; Behavior; Bioinformatics; Biological; Blood - brain barrier anatomy; Breeding; Centers of Research Excellence; Core Facility; Data Analyses; Dedications; Derivation procedure; Development; Edible Plants; Equipment; Extramural Activities; Fee-for-Service Plans; Fostering; Funding; Future; Genomics; Germ-Free; Gnotobiotic; Goals; Histopathology; Housing; Image; Individual; Inflammation; Inflammation Mediators; Infrastructure; Institution; Joints; Knockout Mice; Laboratories; Mentors; Methodology; Mission; Modeling; Mus; National Institute of General Medical Sciences; Pathogenesis; Pathogenicity; Phase; Play; Process; Protocols documentation; Research; Research Personnel; Research Project Grants; Research Support; Resources; Ribosomal DNA; Role; Sampling; Services; System; Techniques; Testing; Tissues; Training; Training and Education; Transgenic Organisms; Translational Research; Universities; Whole Organism; Work; animal facility; cancer immunotherapy; clinical translation; exosome; experimental study; imaging facilities; in vivo imaging system; innovation; intravital microscopy; metabolomics; microbiome research; microbiota; microscopic imaging; morris water maze; mouse model; multi-photon; nanopore; novel; object recognition; programs; protocol development; success; synthetic polymer Bioplex; tool; tumor immunology

Project Summary/Abstract In this Phase 2 of the Functional Microbiomics, Inflammation and Pathogenicity COBRE, the primary goal of the Functional Microbiomics Core (FMC) is unchanged. We will sustain and enhance the well-established integrated service center that functions as a critical resource for the Research Project Leaders (RPLs) as a priority, and secondarily for all investigators of the Center. Since all new COBRE projects also utilize mouse models to explore the inter-relationships between inflammation and pathogenesis, and the contribution of microbiota to this process, our well-established germ-free and gnotobiotic mice facility (GMF) will continue to play a central role in services provided by the FMC. We will continue to provide anaerobic culturing of mixed and/or mono bacterial strains to facilitate the specific colonization of the germ-free mice. 16S ribosomal DNA will be profiled with the in-house developed Oxford nanopore sequencing as well as PacBio-long read sequencing tools, and bioinformatic support provided. Our most extensively used resource; multiplex-based analysis of inflammatory mediators in biological samples, will continue to function using the Bio-Plex 200 (Bio-Rad) multiplex system. In addition, several recent advances allow us to augment the services provided by the FMC. First, the recently developed, highly successful rederivation service of transgenic and KO mouse models as germ-free, will greatly expand the scope and utility of the GMF. Related to this service is the development of a new area of research on the role of microbiota in Alzheimer’s Disease (AD) supported by an administrative supplement to the FMC from NIA/NIGMS. The GMF has already rederived several AD mouse models. For this project, the FMC also developed a suite of mouse behavior analysis models including the Morris Water Maze and Novel Object Recognition tests. These services will support all future ageing related research at the center including a new project on the role of edible-plant derived exosomes in restoring the integrity of the Blood Brain Barrier. We also now include imaging support, in particular Multiphoton Intravital Microscopy for imaging of live tissue and an In Vivo Imaging System (IVIS) for live mouse imaging. Besides functioning as a service center, another major goal of the FMC is to promote the education and training of researchers, primarily the RPLs. The FMC will also interact with other Core facilities at the University of Louisville, such as the Genomics, Metabolomics and Bioinformatics core facilities established with the support of other highly successful COBRE/INBRE programs. In particular, the newly funded Cancer Immunology and Immunotherapy COBRE-operated Functional Immunomics Core (FIC) facility also directed by Dr. Bodduluri has several joint initiatives with the FMC including the establishment of a histopathology service center. The FMC will work with these and other UofL resources in order to avoid duplication of equipment, and to train individual investigators in specific protocols, foster innovative protocol development and integrate analysis of data.

项目摘要/摘要 在功能性微生物学，炎症和致病性cobre的这一阶段2中， 功能性微生物学核心（FMC）没有变化。我们将维持并增强良好的综合 作为研究项目负责人（RPLS）的关键资源的服务中心，优先 其次，适用于中心的所有调查人员。由于所有新的毛茸茸的项目还使用鼠标模型来探索 炎症和发病机理之间的相互关系，以及菌群对此的贡献 过程，我们公认的无菌和gnotobiotic小鼠设施（GMF）将继续在 FMC提供的服务。我们将继续提供混合和/或单细菌的厌氧文化化 促进无菌小鼠特异性定植的菌株。 16S核糖体DNA将与 内部开发了牛津纳米孔测序以及Pacbio-long读取的测序工具， 提供了生物信息学支持。我们最广泛使用的资源；基于多重炎症的分析 生物样品中的介体将继续使用Bio-plex 200（Bio-Rad）多重系统继续运行。在 此外，最近的几项进步使我们能够增加FMC提供的服务。首先，最近 开发的，非常成功的转基因和KO鼠标模型的重新培训服务将极大地将 扩大GMF的范围和效用。与这项服务相关的是开发新的研究领域 关于菌群在阿尔茨海默氏病（AD）中的作用，由FMC的行政补充支持 来自NIA/NIGMS。 GMF已经对几个AD鼠标模型进行了重新介绍。对于这个项目，FMC也 开发了一套小鼠行为分析模型，包括莫里斯水迷宫和新物体 识别测试。这些服务将支持该中心的所有未来与衰老相关的研究 关于可食用植物衍生的外泌体在恢复血脑屏障的完整性中的作用的项目。我们也是 现在包括成像支撑 实时鼠标成像的体内成像系统（IVI）。除了充当服务中心外，另一个主要目标 FMC的旨在促进研究人员的教育和培训，主要是RPL。 FMC也将相互作用 与路易斯维尔大学的其他核心设施，例如基因组学，代谢组学和生物信息学 核心设施在其他非常成功的山脉/INBRE计划的支持下建立。特别是 新资助的癌症免疫学和免疫疗法肌辅助功能免疫学核心（FIC） Bodduluri博士还指导的设施与FMC有多项联合举措，包括建立 组织病理学服务中心。 FMC将使用这些和其他UOFL资源，以避免 设备的重复，并通过特定协议培训单个调查人员，促进创新协议 数据的开发和综合分析。