Functional Microbiomics Core-Bodduluri

功能微生物组学核心-Bodduluri

基本信息

批准号：
10349568
负责人：
HARIBABU BODDULURI
金额：
$ 54.71万
依托单位：
UNIVERSITY OF LOUISVILLE
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-03-01 至 2023-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10349568
关键词：
Address Adopted Anaerobic Bacteria Area Attention Award Bioinformatics Biological Biomedical Research Centers of Research Excellence Clinical Communities Continuing Education Core Facility Data Analyses Development Dietary Intervention Disease Equipment Extramural Activities Fee-for-Service Plans Fostering Funding Generations Genomic DNA Genomics Germ-Free Gnotobiotic Goals Health Housing Human Resources Incubators Individual Infection Inflammation Inflammation Mediators Institution Kentucky Laboratories Mentors Metagenomics Methodology Mission Mus Outcome Pathogenesis Pathogenicity Phase Pilot Projects Process Protocols documentation Research Research Personnel Research Support Resources Ribosomal RNA Sampling Services Sterility Techniques Technology Tissues Training Training and Education Translational Research United States National Institutes of Health Universities Work animal facility base biosafety level 3 facility experimental study fecal transplantation histopathological examination inflammatory marker innovation interest investigator training meetings member metabolomics microbial microbiome microbiome research microbiota mouse model pathogen programs protocol development rRNA Genes social health determinants social relationships success tool

项目摘要

Biomedical research areas including the study of metagenomics, inflammation and pathogenesis are resource and technology intense. All COBRE-supported investigators require state of-the-art resources to successfully compete with investigators from other resource-rich institutions for NIH support. The primary goal of the Functional Microbiomics Core (FMC) is to establish an integrated service center that functions as a critical resource for all Microbiome, Inflammation and Pathogenicity COBRE investigators. Since all the COBRE projects utilize mouse models to explore the inter-relationships between inflammation and pathogenesis, and the contribution of microbiota to this process, the development of a facility to house and maintain germ-free and gnotobiotic mice is crucial to the FMC. An anaerobic culture facility will support research on gnotobiotic mice that requires specific colonization with fecal transplants or defined anaerobic bacteria. Further, in Aim 2, we propose to establish an integrated metagenomics center for microbiota. This facility will prepare genomic DNA following unified protocols and generate 16S rRNA gene amplicon sequences, and provide bioinformatics support for all microbiota analysis. The FMC will also support all COBRE projects with analysis of their biological samples for inflammatory markers as well as histopathological examination of tissue sections. Education and training of junior investigators and the university wide research community in microbiota research is an important goal of the FMC. Towards this goal, the FMC will organize seminars as well as informal meetings that will serve as a forum for highlighting the scientific and technical advances in the field. Finally, the FMC plans to become an independent service center at UofL to support the needs of all researchers in this area of research

生物医学研究领域，包括宏基因组学、炎症和发病机制的研究都是资源和技术密集。所有 COBRE 支持的调查人员都需要最先进的资源才能成功与其他资源丰富机构的研究人员竞争 NIH 的支持。该组织的首要目标是功能微生物组学核心（FMC）是建立一个综合服务中心，作为关键的功能微生物组学核心为所有微生物组、炎症和致病性 COBRE 研究人员提供的资源。由于所有 COBRE 项目利用小鼠模型探索炎症和发病机制之间的相互关系微生物群对此过程的贡献，开发容纳和保持无菌和无菌小鼠对于 FMC 至关重要。厌氧培养设施将支持对无菌小鼠的研究需要粪便移植或特定的厌氧细菌进行特定定植。此外，在目标 2 中，我们建议建立微生物群综合宏基因组学中心。该设施将在以下情况下制备基因组 DNA 统一实验方案并生成16S rRNA基因扩增子序列，为大家提供生物信息学支持微生物群分析。 FMC 还将支持所有 COBRE 项目，对其生物样本进行分析，以用于炎症标志物以及组织切片的组织病理学检查。青少年教育与培训研究人员和大学范围内的微生物群研究是 FMC 的一个重要目标。为了实现这一目标，FMC 将组织研讨会和非正式会议，作为论坛突出该领域的科学技术进步。最后，FMC 计划成为一个独立的组织伦敦大学服务中心，支持该研究领域所有研究人员的需求