Center for Live Cell Genomics

活细胞基因组学中心

基本信息

批准号：
10676332
负责人：
DAVID H HAUSSLER
金额：
$ 207.38万
依托单位：
UNIVERSITY OF CALIFORNIA SANTA CRUZ
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-15 至 2026-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10676332
关键词：
3-Dimensional 3D Print Address Area Biological Assay Biopsy Bioreactors Blood Brain Brain Injuries CRISPR interference CRISPR-mediated transcriptional activation Cancer Patient Cell Culture Techniques Cell Line Cells Cellular Phone Cellular biology Cerebrum Childhood Glioma Clinical Data Clustered Regularly Interspaced Short Palindromic Repeats Code Communities Competence Complementary DNA Computer software Computers Creativeness Data Data Analyses Data Set Democracy Development Devices Disadvantaged Disease Disease model Drug Screening Ecosystem Education Education and Outreach Educational workshop Electrodes Ensure Environment Evolution Feedback General Population Genes Genomic Data Commons Genomic approach Genomics Genotype Geometry Growth Human Hydrogels Image Incubators Individual Institution Intercellular Fluid International Internet Internet of Things Laboratories Link Machine Learning Maintenance Malignant Childhood Neoplasm Malignant Neoplasms Maps Membrane Potentials Mentorship Methodology Microfluidics Microscopy Modeling Molds Molecular Monitor Morphology National Human Genome Research Institute Neurobiology Neurodevelopmental Disorder Neurosciences Research Operative Surgical Procedures Organoids Pathogenesis Pathway interactions Patients Pattern Pediatric Oncology Performance Perfusion Phenotype Pongidae Postdoctoral Fellow Printing Productivity Proteins RNA Reporter Reproducibility Research Research Personnel Resolution Resources Schools Science Scientist Secondary Schools Speed System Techniques Technology Testing Therapeutic Tissue Model Tissues Training Training Programs Universities Variant Vascularization Venous Work anticancer research base cell type cohort community college cost data sharing design doctoral student driver mutation education access empowerment engineered stem cells exosome experimental study fabrication flexibility genome sciences genomic data glioma cell line graduate student improved innovation interest nanopore nervous system disorder neural circuit neurodevelopment new technology next generation novel strategies online community online resource open source optogenetics outreach pedagogy petabyte pre-clinical precision oncology programs remote control response scaffold screening sensor stem cells stroke model student training three dimensional cell culture tissue culture tissue/cell culture transcriptomics tumor undergraduate student webinar

项目摘要

ABSTRACT Center for Live Cell Genomics We will build new methodology and capacity for large-scale, long-term, inexpensive, modular, customizable, shared, Internet-of-Things-controlled, reproducible live cell culture and tissue-based experimental genomics disease models. Tissue models include traditional cell culture as well as organoid and primary tissue explants obtained from surgery or biopsy. Organoid factories supporting tissue growth and maintenance will be integrated with external and on-chip electro-optofluidic analytical modules to become part of an ecosystem that is modeled after open-source software. It will use commodity sensors, cameras, and computers linked in platforms flexibly designed using simple 3D printing, molding, etching and milling techniques potentially available at any institution. This will stimulate rapid innovation in experimental platforms for tissue culture. We will push this technology and use its best-in-class capabilities to make progress in neurodevelopment and pediatric cancer, addressing big questions. What genes contribute most importantly and specifically to human brain development? How do they go wrong in neurodevelopmental disease or brain injury? What specific molecular pathways are disrupted in individual pediatric cancer cases? How can we test pathway-specific treatments in a tissue model specific to each patient? Our education and outreach plans include a training program to develop a diverse and inclusive cohort of undergraduate students trained in genomic science through secondary school and community college outreach as well as coding workshops and research-based laboratory classes for UCSC undergraduates to develop core competencies. Participation in these activities serve as a basis for training graduate students and postdocs in inclusive pedagogy and mentorship. We are also developing a one-stop information hub to form an online community and to share our technology through immersive webinars and tutorials aimed at a broader audience with a range of expertise from the general public to scientists and clinicians at research institutions. Our work will enable significant advances in neuroscience and cancer research and education, stimulate a new open-source culture in cell biology and genomics, and democratize scientific and educational access beyond elite institutions, extending sharing projects like NHGRI ANvIL beyond data and code to include experiments and Internet-connected experimental platforms.

抽象的活细胞基因组学中心我们将为大规模、长期、廉价、模块化、可定制、共享、物联网控制、可重复的活细胞培养和基于组织的实验基因组学疾病模型。组织模型包括传统的细胞培养以及从手术或活检获得的类器官和原代组织外植体。类器官工厂配套组织生长和维护将与外部和片上光电流体分析集成模块成为以开源软件为模型的生态系统的一部分。它将使用使用简单 3D 灵活设计的平台连接商品传感器、相机和计算机任何机构都可以使用印刷、模制、蚀刻和铣削技术。这将刺激组织培养实验平台的快速创新。我们将推动这项技术并利用其一流的能力在以下方面取得进展神经发育和儿童癌症，解决重大问题。什么基因贡献最大对于人类大脑的发育来说是重要且具体的吗？他们是如何出错的神经发育疾病或脑损伤？哪些特定的分子途径被破坏个别儿童癌症病例？我们如何在组织模型中测试途径特异性治疗具体到每个患者？我们的教育和推广计划包括培养多元化和包容性群体的培训计划通过中学和社区接受基因组科学培训的本科生 UCSC 的大学外展以及编码研讨会和基于研究的实验室课程本科生培养核心能力。参与这些活动是对研究生和博士后进行包容性教学和指导方面的培训。我们也在开发一站式信息中心，形成在线社区并通过以下方式分享我们的技术沉浸式网络研讨会和教程，面向更广泛的受众，提供来自业界的一系列专业知识公众、研究机构的科学家和临床医生。我们的工作将使神经科学和癌症研究和教育取得重大进展，刺激细胞生物学和基因组学的新开源文化，并使科学和技术民主化超越精英机构的教育机会，将 NHGRI ANvIL 等共享项目扩展到数据之外以及包含实验和互联网连接实验平台的代码。