Modernization of 3-dimensional printing capabilities at the Aquatic Germplasm and Genetic Resource Center

水产种质和遗传资源中心 3 维打印能力的现代化

• 批准号: 10736961
• 负责人: Terrence Robert Tiersch
• 金额: $ 22.89万
• 依托单位: LOUISIANA STATE UNIV AGRICULTURAL CENTER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10736961
• 关键词: 3-Dimensional; 3D Print; Address; Adopted; Animals; Basic Science; Biomedical Research; Collaborations; Communities; Computers; Consumption; Cryopreservation; Custom; Defect; Development; Devices; Economics; Electrodes; Electronics; Engineering; Equipment; Faculty; Filament; Freezing; Funding; Genes; Genetic Transformation; Genetic study; Germ; Goals; Health; Human; Individual; Industrialization; Inferior; Infrastructure; Institution; Interdisciplinary Study; International; Investments; Knock-out; Laboratories; Maintenance; Metals; Microfabrication; Modeling; Modernization; Pathway interactions; Plant Resins; Postdoctoral Fellow; Prevention; Printing; Procedures; Process; Production; Protocols documentation; Quality Control; R24; Recovery; Reproducibility; Research; Research Personnel; Research Project Grants; Resource Sharing; Resources; Risk; Sampling; Science; Services; Site; Standardization; System; Technology; Time; Transgenic Organisms; United States Department of Agriculture; United States National Institutes of Health; Work; Zebrafish; agricultural center; aquatic organism; collaborative environment; commercialization; cost; genetic resource; graduate student; improved; innovative technologies; interdisciplinary approach; manufacturing technology; mechanical device; mutant; novel; open source; portability; preservation; prevent; programs; quality assurance; repository; research and development; screening; sensor; skills; technology development; tool; undergraduate student

PROJECT SUMMARY/ABSTRACT: Aquatic species have become accepted as valuable models in biomedical research, and laboratories around the world produce tens of thousands of mutant, knockout, gene-edited and transgenic lines each year. Establishment of efficient and reliable germplasm repositories is critical for preservation of genetic resources of aquatic organisms that are vital to advancing biomedical research. However, a pervasive lack of reproducibility in cryopreservation creates a significant barrier, posing a great risk of losing valuable lines developed from billions of dollars of research investment. There are two major problems: first, although protocols have been established through basic research, laboratories often produce low-quality samples because of a lack of affordable and reliable hardware for a standardized production pathway. Second, there are no effective approaches to provide quality management at a system level, including quality assurance for prevention of defects, and quality control for elimination of inferior products. Although some commercial options exist, most laboratories are not able to purchase expensive equipment when germplasm banking is not a focus or obligation. In previous R24 projects we worked with the Zebrafish International Resource Center (ZIRC) to establish strong on-site capabilities for them as a central facility. We also developed mechanical devices through 3-D printing and microfabrication to begin standardization efforts at ZIRC. We are expanding this technology initiative for widespread use across the steps required for comprehensive production and quality management pathways by development of multiple types of new scientific hardware that are practical, inexpensive, standardizable, portable, and customizable, and can be made available as user-driven public toolboxes through open hardware for thousands of laboratories. Over the past 10 years the AGGRC, a shared- use facility, has become a leader in production of open hardware for cryopreservation and repository development. To date, we have primarily restricted our work to consumer-level equipment to ensure that hardware distributed as printable computer files would be available to all users regardless of skill level. Although we are well-equipped for filament-based (e.g., plastic) 3-D printing, we have great need to substantially improve our ability to 3-D print with photocurable resins and metal. Modernized equipment is essential for a sustained powerful influence in this field. For example, we have adopted resin printing as an alternative to expensive and time-consuming microfabrication (e.g., photolithography), and require metal printing to work with resins to fabricate tools such as custom electrodes and sensors. Modernizing our capabilities will greatly streamline and improve operating processes and procedures, and it will provide much- needed access to technologically advanced equipment to support current, rapidly evolving, and emerging research programs. This will enable new and advanced approaches, offer innovative technological solutions, and benefit user communities and multiple research projects and investigators at our institution and beyond.

项目概要/摘要： 水生物种已被认为是生物医学研究和周围实验室的有价值的模型 世界每年产生数以万计的突变、敲除、基因编辑和转基因品系。 建立高效、可靠的种质资源库对于保存遗传资源至关重要 对推进生物医学研究至关重要的水生生物。然而，普遍缺乏可重复性 冷冻保存中产生了一个重大障碍，造成了失去从其开发的有价值的细胞系的巨大风险 数十亿美元的研究投资。有两个主要问题：第一，虽然协议已经制定 通过基础研究建立起来的实验室经常由于缺乏 用于标准化生产途径的经济实惠且可靠的硬件。二是没有有效的办法 在系统层面提供质量管理的方法，包括预防质量保证 缺陷和质量控制，以消除劣质产品。尽管存在一些商业选择，但大多数 当种质库不是重点时，实验室无法购买昂贵的设备，或者 义务。在之前的 R24 项目中，我们与斑马鱼国际资源中心 (ZIRC) 合作， 为他们建立强大的现场能力作为中心设施。我们还开发了机械设备 通过 3D 打印和微加工，ZIRC 开始标准化工作。我们正在扩展这个 技术倡议在全面生产和质量所需的步骤中广泛使用 通过开发多种实用的新型科学硬件来管理途径， 廉价、可标准化、可移植、可定制，并且可以作为用户驱动的公共资源提供 通过开放硬件为数千个实验室提供工具箱。在过去的 10 年里，AGGRC 是一个共享的 使用设施，已成为低温保存和存储库开放硬件生产的领导者 发展。迄今为止，我们主要将工作限制在消费级设备上，以确保 作为可打印计算机文件分发的硬件将可供所有用户使用，无论其技术水平如何。 尽管我们已经具备了基于线材（例如塑料）的 3D 打印的装备，但我们仍然非常需要 大幅提高我们使用光固化树脂和金属进行 3D 打印的能力。现代化设备是 对于在该领域保持持续强大的影响力至关重要。例如，我们采用树脂印刷作为 替代昂贵且耗时的微加工（例如光刻），并且需要金属 打印与树脂一起制造定制电极和传感器等工具。现代化我们的 能力将极大地简化和改进操作流程和程序，并且它将提供很多- 需要获得技术先进的设备来支持当前、快速发展和新兴的 研究计划。这将实现新的先进方法，提供创新的技术解决方案， 并使我们机构内外的用户社区以及多个研究项目和研究人员受益。