Tagged reagents for biological Processes

用于生物过程的标记试剂

• 批准号: 7734329
• 负责人: William Trenkle
• 金额: $ 25.33万
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7734329
• 关键词: Acetylglucosamine; Animal Disease Models; Biological; Biological Assay; Biological Process; Biological Testing; Calmodulin; Cell physiology; Cells; Chemical Structure; Class; Cleaved cell; Core Facility; Development; Diabetes Mellitus; Discrimination; Disruption; Enzymes; Facility Construction Funding Category; Fluorescein; Fluoresceins; Future; Link; Mammalian Cell; Methods; Modification; Monosaccharides; Nerve Degeneration; Non-Insulin-Dependent Diabetes Mellitus; Nuclear; Numbers; O-GlcNAc transferase; Pathway interactions; Phospholipase C; Phosphorylation; Positron-Emission Tomography; Post-Translational Protein Processing; Preparation; Protein Dephosphorylation; Proteins; Purinergic P1 Receptors; Purpose; Radiopharmaceuticals; Reagent; Screening procedure; Series; Signal Pathway; Signal Transduction; Specificity; System; Testing; base; computerized data processing; desire; drug development; improved; insulin signaling; interest; intracellular protein transport; protein localization location; trafficking

During FY2007, a series of substrates was prepared for biological testing for several diverse projects. These preparations required the development of new methods for construction and purification of the chemical structures required for the assays. Biological studies are currently underway to delineate the future directions of the various aspects of this project. The projects collaboratively supported by the core facility include: Z01 DK060101-02 -- Targeted disruption the enzymes of O-GlcNAc cycling: Animal models of Disease Z01 DK060102-02 -- Calmodulin-driven Nuclear Trafficking linked to diabetes and insulin signaling Z01 DK060103-02 -- Enzymes of O-GlcNAc cycling linked to type-2 diabetes and neurodegeneration Z01 DK031117-20 -- Development Of Drugs Acting At Adenosine Receptors Z01 EB000001-04 -- New Radiopharmaceuticals and New Paradigms in Positron Emission Tomography

在2007财年期间，为几个不同项目的生物测试准备了一系列底物。 这些准备工作需要开发新方法来构建和纯化测定法所需的化学结构。 目前正在进行生物学研究，以描绘该项目各个方面的未来方向。 核心设施协作支持的项目包括： Z01 DK060101-02-靶向破坏O-GlcNAC自行车的酶：疾病动物模型 Z01 DK060102-02-与糖尿病和胰岛素信号传导有关的钙调蛋白驱动的核运输 Z01 DK060103-02-与2型糖尿病和神经退行性相关的O-GLCNAC循环酶 Z01 DK031117-20-作用在腺苷受体上的药物的开发 Z01 EB000001-04-正电子发射断层扫描中新的放射性药物和新范式