Cell Biology

细胞生物学

• 批准号: 10669728
• 负责人: Jun Yang
• 金额: $ 15.09万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10669728
• 关键词: Area; Award; Basic Science; Cell Culture Techniques; Cells; Cellular biology; Collaborations; Consultations; Cryopreservation; Cryoultramicrotomy; Disease; Evolution; Faculty; Future; Generations; Genotype; Glaucoma; Individual; Laboratories; Mus; Organoids; Paraffin Embedding; Research; Research Personnel; Research Project Grants; Resources; Retina; Retinal Degeneration; Services; System; Technical Expertise; Training; Translational Research; Universities; Utah; Vision research; developmental disease; eye center; immunocytochemistry; induced pluripotent stem cell; mouse genetics; preservation; tissue culture; working group

Project Summary-Cell Biology Core The OVERALL aims of this Vision Research Core (VRC) are to provide: • access to resources outside the scope of individual R01 awards • access to technical expertise outside the scope a single laboratory • staff training to remove barriers to efficient translational research and collaboration • collaboration initiatives among VRC labs The research areas supported by the VRC span the analysis and treatments of retinal degenerations, developmental disorders, glaucoma and other disorders, as well as a range of cutting-edge basic science initiatives. We have implemented four resource modules that continue the natural evolution of how this research group works together, serving 17 investigators holding 22 NEI R01 awards. The Cell Biology Module derives from the strengths in mouse genetics at the Moran Eye Center and the high demand for tissue culture and profiling. Almost every JMEC laboratory has need of high quality resources for cryosectioning, paratoming, genotyping, immunocytochemistry, quantitative PCR (qPCR), cell culturing, and mouse colony preservation. Some JMEC laboratories will have future need for induced pluripotent stem cell (iPSC) generation and retinal organoid differentiation. Specifically the Cell Biology module provides: • High-quality cryotomy resources through two professionally maintained Leica CM3050S systems • Paraffin embedding and section services via a Leica Paratome • PCR and genotyping services • Training of cryosectioning, immunocytochemistry, genotyping, and qPCR • Cell culture resources through access to a well-equipped and maintained biosafety level 2 (BSL2) cell culture facility • Technical consultation for mouse line cryopreservation, iPSC generation and retinal organoid differentiation

项目概要-细胞生物学核心 该视觉研究核心 (VRC) 的总体目标是提供： • 获取个人R01 奖项范围之外的资源 • 获得单一实验室范围之外的技术专业知识 • 员工培训，以消除高效转化研究和协作的障碍 • VRC 实验室之间的合作计划 VRC 支持的研究领域涵盖视网膜变性的分析和治疗、 发育障碍、青光眼等疾病以及一系列前沿基础科学 我们已经实施了四个资源模块，以继续这一过程的自然演变。 研究小组共同努力，服务了 17 名研究人员，获得了 22 个 NEI R01 奖项。 细胞生物学模块源自莫兰眼科中心小鼠遗传学的优势和高水平 组织培养和分析的需求几乎每个 JMEC 实验室都需要高质量的资源。 冷冻切片、切片、基因分型、免疫细胞化学、PCR (qPCR)、细胞定量培养和 一些 JMEC 实验室未来将需要诱导多能干细胞。 (iPSC) 生成和视网膜类器官分化。 具体来说，细胞生物学模块提供： • 通过两个专业维护的 Leica CM3050S 系统提供高质量的冷冻术资源 • 通过 Leica Paratome 进行石蜡包埋和切片服务 • PCR 和基因分型服务 • 冷冻切片、免疫细胞化学、基因分型和 qPCR 培训 • 通过使用设备齐全且维护良好的生物安全 2 级 (BSL2) 细胞来获取细胞培养资源 文化设施 • 小鼠系冻存、iPSC生成和视网膜类器官技术咨询 差异化