UCD KOMP Project Repository

UCD KOMP 项目存储库

• 批准号: 7788345
• 负责人: KC KENT LLOYD
• 金额: $ 13.89万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-20 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7788345
• 关键词: Alleles; Archives; Arts; Biological Preservation; Biology; Businesses; California; Cataloging; Catalogs; Collection; Communities; Data; Data Coordinating Center; Databases; ES Cell Line; Electronic Mail; Embryo; Ensure; Faculty; Fees; Freezing; Gene Targeting; Genotype; Goals; Grant; Health Status; Individual; Informatics; Institution; International; Intracytoplasmic Sperm Injections; Karyotype; Knockout Mice; Lead; Life; Link; Measures; Medicine; Microinjections; Mus; Pathology; Pediatric Hospitals; Plasmid Cloning Vector; Procedures; Production; Protocols documentation; Quality Control; Recovery; Reporting; Repository Operations; Research; Research Infrastructure; Research Institute; Research Personnel; Resources; Services; System; Telephone; Testing; Universities; Veterinary Medicine; Vial device; authority; comparative; cost; embryonic stem cell; experience; medical schools; mutant; pathogen; programs; repository; sperm cell; tool; transmission process; vector; web site

DESCRIPTION (provided by applicant): This proposal from the University of California, Davis (UCD) and its subcontractor at CHORI seeks to establish and operate the Knock-Out Mouse Program (KOMP) Repository for the purpose of ensuring the preservation, protection, availability, and accessibility of the KOMP resources for use by the scientific research community. The repository will be built upon the closely linked infrastructure, technical support, and faculty expertise within the UCD Mouse Biology Program, Center for Comparative Medicine, the Schools of Medicine and Veterinary Medicine, and the CHORI BACPAC Resource. UCD shall serve as the lead institution and assume administrative oversight, management, and reporting authority and responsibility for all repository activities. UCD shall archive, maintain, QC, and distribute ES cell clones, live mouse lines, and frozen embryos and sperm, while CHORI shall archive, maintain, QC, and distribute all vectors. Our goals will be to 1) to verify the viability, identity, and utility of the collection; 2) make finding and ordering all KOMP products and value-added services simple and easy on-line; and 3) distribute KOMP products promptly and for reasonable cost. To achieve these goals, we shall 1) expand 100% of all gene-targeted ES cell lines to 12-15 vials each; 2) genotype, karyotype, and pathogen-screen ES cell clones in each shipment; 3) reanimate 425 clones to live mice and test for germline transmission of the mutant allele; 4) convert 925 mouse lines (~11% of the collection) to a cryopreserved embryo and sperm archive, and 5) confirm the genotype and SPF-status of each clone when ordered. Requesting investigators will have full access to all UCD resources and services, including reanimation of ES cells into live mice, etc. We shall coordinate our relational databases, internally track KOMP products, interface with the Data Coordinating Center, maintain an easily-navigable and informative public website (www.komp.org), and provide an online searchable catalog and ordering system for all KOMP products, services, and related information. We shall provide attentive customer and technical service to respond to telephone (1-888-KOMP-MICE) and email ([e-mail]) requests for information. Products and services will be offered to requesting investigators for a reasonable fee, enabling us to cost recover for distribution and to develop a self supporting repository operation within 4 years.

描述（由申请人提供）：加利福尼亚大学戴维斯大学（UCD）及其在Chori的分包商的这一提案旨在建立和运营淘汰鼠标计划（KOMP）存储库，以确保科学研究社区使用KOMP资源的保存，保护，可用性，可用性和可访问性。该存储库将建立在UCD小鼠生物学计划，比较医学中心，医学和兽医学院以及Chori Bacpac资源的紧密联系基础设施，技术支持和教师专业知识上。 UCD应作为主要机构，并承担所有存储库活动的行政监督，管理和报告权限和责任。 UCD应存档，维护，QC和分发ES细胞克隆，活小鼠线以及冷冻的胚胎和精子，而Chori应存档，维护，QC并分布所有向量。我们的目标将是1）验证收集的生存能力，身份和效用； 2）使查找和订购所有KOMP产品和增值服务简单简便； 3）及时分发Komp产品，以合理的成本。为了实现这些目标，我们应1）将所有靶向基因的ES细胞系的100％扩展到12-15个小瓶； 2）每个发货中的基因型，核型和病原体屏幕ES克隆； 3）复兴425个克隆，以实现小鼠并测试突变等位基因的生殖线传播； 4）将925条小鼠线（约11％的收集）转换为冷冻保存的胚胎和精子档案，5）确认每个克隆时的基因型和SPF状态。请求调查人员将完全访问所有UCD资源和服务，包括将ES细胞重新启用到活鼠等。我们将协调我们的关系数据库，内部跟踪Komp产品，与数据协调中心的交互，维护易于畅通且有用的公共网站（www.komp.org）（www.komp.org），并为在线搜索搜索范围内的产品和订购系统，并提供各种服务。我们将提供专注的客户和技术服务，以响应电话（1-888-komp-c）和电子邮件（[e-mail]）请求信息。产品和服务将提供给调查人员的合理费用，使我们能够在4年内进行分发的费用，并在4年内开发自支持的存储库操作。