Spike-triggered current source-density analysis: The synaptic impact of a single

尖峰触发电流源密度分析：单个电流的突触影响

• 批准号: 7760573
• 负责人: HARVEY A SWADLOW
• 金额: $ 19.13万
• 依托单位: UNIVERSITY OF CONNECTICUT STORRS
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-02-01 至 2011-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7760573
• 关键词: Abbreviations; Area; Axon; Beds; Behavior Disorders; Brain; Caliber; Cell Communication; Cells; Characteristics; Collaborations; Communication; Communities; Complex; Contralateral; Data; Development; Disease; Distant; Electric Stimulation; Electrodes; Elements; Environment; Event; Felis catus; Figs - dietary; Future; Goals; Human; Image; In Vitro; Interneurons; Knowledge; Label; Lateral Geniculate Body; Measures; Mental Health; Methods; Modeling; Monitor; Morphology; Neurons; Oryctolagus cuniculus; Penetration; Physiologic pulse; Population; Psyche structure; Running; Slice; Somatosensory Cortex; Source; Synapses; System; Testing; Thalamic structure; Time; Training; Visual Cortex; Work; awake; base; biocytin; density; extracellular; hippocampal pyramidal neuron; in vivo; interest; postsynaptic; presynaptic; professor; relating to nervous system; response; tool

DESCRIPTION (provided by applicant): The hallmark of neuronal systems lies in the vast, modifiable functional interconnections among the elements. Understanding brain function requires a detailed knowledge of how such functional connectivity is achieved, but the tools for analysis of synaptic communication in the waking brain are very limited. In vitro, cell-to-cell connectivity has been studied using paired intracellular recordings or minimal electrical stimulation of thalamic or other afferents. These methods have yielded a wealth of information about cell-to-cell interactions, but in vitro slices eliminate many of the synaptic and modulatory inputs that operate in intact brains. Moreover, these methods are difficult to apply in vivo, where the primary tool for examining synaptic interactions has been extracellular cross-correlation. The above in vitro tools, as well as in vivo extracellular cross-correlation, generally yield an analysis of the synaptic impact of a single presynaptic neuron on a single (or on a very few) postsynaptic target cells. However, some experimental questions require a view of the overall, global synaptic impact generated by a single presynaptic neuron on its targeted cortical domain. We have been developing a method that yields such a view, referred to as spike-triggered current source-density analysis. This method allows an analysis of the synaptic impact generated by a single presynaptic neuron on a neuronal population contained within a restricted cortical cylinder of ~200 microns diameter. This method has proved very useful in the study of systems in which the presynaptic neuron generates a relatively powerful and focal impact on the targeted domain. The goal of the proposed work is to gain a better understanding of both the strengths and limitations of this method, and to extend its use to weaker and less focal neuronal connections, which are more prevalent in the brain. By doing so, the proposed work will provide the scientific community with a new and powerful tool for the study of functional connectivity in the intact, awake brain. The current work will have an important impact on our ability to monitor functional neuronal connectivity in intact, awake brains. A disruption in neural communication has been associated with a wide range of mental diseases. This proposal involves development of new methods to study communication between neurons and will provide the basis for future studies of human mental health and behavioral disorders.

描述（由申请人提供）：神经元系统的标志在于元素之间广泛的，可修改的功能互连。了解大脑功能需要详细了解如何实现这种功能连接性，但是分析醒来的大脑突触通信的工具非常有限。在体外，已经使用成对的细胞内记录或最小的丘脑或其他传入的电刺激研究了细胞对细胞连接性。这些方法产生了有关细胞间相互作用的大量信息，但是体外切片消除了在完整的大脑中运行的许多突触和调节输入。此外，这些方法很难在体内应用，其中用于检查突触相互作用的主要工具是细胞外互相关的。上面的体外工具以及体内的细胞外互相关，通常对单个突触前神经元对单个（或极少数）突触后靶细胞的突触影响进行分析。但是，某些实验问题需要查看单个突触前神经元对其靶向皮质结构域产生的整体全局突触影响。我们一直在开发一种产生这种视图的方法，称为尖峰触发的当前源密度分析。该方法允许分析单个突触前神经元对〜200微米直径约200微米的受限皮层圆柱体中包含的神经元种群产生的突触影响。事实证明，该方法在研究系统前神经元对靶向域产生相对强大和焦点的影响非常有用。拟议的工作的目的是更好地了解该方法的优势和局限性，并将其使用扩展到较弱的局灶性神经元连接，这在大脑中更为普遍。通过这样做，拟议的工作将为科学界提供一种新的强大工具，以研究完整，清醒大脑中功能连接性的研究。当前的工作将对我们监测完整，清醒大脑中功能性神经元连接的能力有重要影响。神经交流的破坏与广泛的精神疾病有关。该建议涉及开发新方法来研究神经元之间的交流，并将为人类心理健康和行为障碍的未来研究提供基础。

项目成果

Faster thalamocortical processing for dark than light visual targets.

丘脑皮质对暗视觉目标的处理速度比对亮视觉目标的处理速度更快。

• DOI: 10.1523/jneurosci.2456-11.2011
• 发表时间: 2011-11-30
• 期刊: The Journal of neuroscience : the official journal of the Society for Neuroscience
• 作者: Jin J;Wang Y;Lashgari R;Swadlow HA;Alonso JM
• 通讯作者: Alonso JM