Flexible, open source software for laser scanning microscopy

用于激光扫描显微镜的灵活开源软件

• 批准号: 7691935
• 负责人: Gordon M Shepherd
• 金额: $ 32.96万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-06-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7691935
• 关键词: Applications Grants; Area; Biological; Biomedical Research; Birds; Chicago; Collection; Communities; Complex; Computer software; Computers; Data; Direct Costs; Documentation; Electronic Mail; Electrophysiology (science); Facilities and Administrative Costs; Floor; Foundations; Funding; Future; Grant; Illinois; Image; Laboratories; Laos; Laser Scanning Microscopy; Lasers; Light; Maps; Measurement; Medical center; Methods; Microscope; Microscopy; Neurosciences; Photons; Physiology; Play; Principal Investigator; Public Health; Research; Role; Scanning; Software Tools; Source; Techniques; Tin; Universities; Vertebral column; Video Recording; Writing; analog; base; cost; data acquisition; detector; digital; flexibility; improved; in vivo; interest; medical schools; neural circuit; open source; professor; programs; tool; ward; wiki

(Enter the text here that is the new abstract information for your application. This section must be no longer than 30 lines of text.) C.) Laser scanning microscopy (LSM), including confocal and 2-photon microscopy, continues to play a central role in many areas of biomedical research. In neuroscience alone, the number of applications continues to grow rapidly. However, in contrast to the ongoing advances made on the hardware aspects of LSM (eg. improved, cheaper light sources, detectors, and scanners), a major limitation to using LSM continues to be the cost and inflexibility of commercial software. Several years ago we introduced Scanlmage, an open source software package for LSM. Scanlmage has been highly successful and is now used by many labs, in particular for 2-photon microscopy. However, new types of cutting-edge applications require new functionality. In addition, it is usually important to combine LSM with other types of digital or analog stimulation or acquisition, such as electrophysiology, underscoring a related, more fundamental need, for an open-source general-purpose data acquisition program. Here, we propose (1) to continue to develop Scanlmage, with a focus on in vivo imaging and (2) to develop Ephus, a new and complementary program for general-purpose laser scanning and data acquisition, such as electrophysiological recording and video imaging. These programs are mostly written in Matlab, with an underlying C-backbone. Future releases of Scanimage will support new types of scanning hardware; improved region-of-interest and related scans; fast z-scanning; and, improved on-line multicolor display. Future releases of Ephus will include support for COD camera -based image acquisition and tools to facilitate easy creation of complex experimental paradigms, such as laser scanning photostimulation based mapping of neural circuits, We will maintain wiki-style documentation for Scanimage and Ephus. We believe that Scanlmage/Ephus will facilitate exciting biological discovery in numerous laboratories. v0, (l) 0-0 CAA ,-,. (n� .(D 0(Q --� N-0

（在此处输入文本，这是新的抽象信息 您的申请。本节必须不超过30行文本。） C.） 激光扫描显微镜（LSM），包括共聚焦和2光子显微镜，在许多生物医学研究领域继续起着核心作用。仅在神经科学中，应用的数量继续迅速增长。但是，与LSM硬件方面的持续进展相比（例如，改进的，廉价的光源，探测器和扫描仪），使用LSM的主要限制仍然是商业软件的成本和僵化性。几年前，我们引入了ScanlMage，这是一个用于LSM的开源软件包。扫描量非常成功，现在许多实验室使用，特别是用于2光子显微镜。但是，新型的尖端应用程序需要新的功能。此外，对于开源通用数据采集计划，将LSM与其他类型的数字或模拟刺激或获取相结合，例如电生理学，强调相关，更根本的需求。在这里，我们建议（1）继续开发扫描量，重点是体内成像，（2）开发Ephus，Ephus是一种用于通用激光扫描和数据获取的新的互补程序，例如电生理记录和视频成像。这些程序主要用MATLAB编写，并带有基础C-Backbone。未来的扫描版本将支持新型的扫描硬件类型；改善利益区域和相关扫描；快速Z扫描；并且，改进了在线多色显示屏。 Ephus的未来释放将包括对基于COD相机的图像采集的支持和工具，以促进容易创建复杂的实验范式，例如基于激光扫描基于神经回路的光刺激映射，我们将维护Wiki型扫描和Ephus的文档。我们认为，Scanlmage/Ephus将以许多方式实验室促进令人兴奋的生物学发现。 v0， （L） 0-0 CAA ， - ，。 （n 。（d 0（q - � N-0