The role of Fbxl22 in the regulation of skeletal muscle mass

Fbxl22在骨骼肌质量调节中的作用

• 批准号: 10414945
• 负责人: David C Hughes
• 金额: $ 12.04万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-01 至 2023-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10414945
• 关键词: Acute; Address; Affect; Aging; Atrophic; Chronic Disease; Complex; Data; Denervation; Direct Lytic Factors; Disease; Electroporation; Exercise; F Box Domain; Faculty; Family member; Fasting; Genes; Genetic; Genetic Transcription; Hindlimb; Homeostasis; Hospitalization; Human Genome; Hypertrophy; Individual; Injury; Iowa; Leucine; Mediating; Mentored Research Scientist Development Award; Mentors; Modeling; Molecular; Muscle; Muscle Fibers; Muscle Proteins; Muscle function; Muscular Atrophy; Myocardium; Myogenic Regulatory Factors; Myogenin; Myopathy; Nerve Crush; Persons; Phenotype; Plasmids; Play; Positioning Attribute; Post-Translational Protein Processing; Process; Promoter Regions; Protein Isoforms; Proteins; Proteomics; Quality of life; RNA Interference; Recording of previous events; Regulation; Research; Research Personnel; Role; Skeletal Development; Skeletal Muscle; Stimulus; Structure; System; Techniques; Testing; Therapeutic; Therapeutic Intervention; Tissues; Ubiquitin; Ubiquitination; Universities; alpha Actinin; base; filamin; genetic manipulation; improved; in vivo; knock-down; leucine-rich repeat protein; mRNA Expression; multicatalytic endopeptidase complex; muscle form; muscle physiology; myogenesis; novel; novel therapeutic intervention; overexpression; protein degradation; skeletal muscle wasting; skills; tool; treatment strategy; ubiquitin-protein ligase

Project Summary/Abstract Skeletal muscle atrophy occurs as a consequence of many chronic diseases and conditions such as disuse. Central to the process of muscle atrophy is protein degradation, for which the E3 ubiquitin ligases are critical in targeting specific proteins for degradation. The roles of many E3 ubiquitin ligases in muscle are unknown and specific substrates have yet to be identified. The overall objective of this proposal is to determine the role of the E3 ligase, F-box and leucine-rich repeat protein 22 (Fbxl22), in skeletal muscle atrophy. My central hypothesis is that Fbxl22 is necessary for the process of muscle atrophy. Robust preliminary data supports this hypothesis: 1) Fbxl22 mRNA expression is induced after 3 days in a model of neurogenic muscle atrophy, 2) Overexpression of Fbxl22 in hindlimb muscles results in significant elevations of total ubiquitinated proteins. I propose two specific aims: Specific Aim 1: Determine the functional role of Fbxl22 isoforms in skeletal muscle atrophy, injury and regrowth. I will address this aim using knockdown and overexpression of Fbxl22 gene isoforms in a model of neurogenic muscle atrophy and assess Fbxl22 isoform expression in an acute muscle injury model. Specific Aim 2: To identify substrate targets for Fbxl22-dependent ubiquitination. I will address this aim using a combined approach of Fbxl22 gene manipulation with proteomics analysis and post-translational modification identification of ubiquitin motifs on Fbxl22-dependent substrates. With the completion of this proposal, I expect to have identified novel mechanisms for understanding the muscle atrophy process. By identifying the mechanisms of skeletal muscle atrophy, we can explore new therapeutic strategies for improving an individual’s quality of life where muscle wasting is present. The University of Iowa has a longstanding history of support to its trainees and junior faculty. My mentor, Prof. Bodine is a world leader in skeletal muscle physiology, atrophy and hypertrophy. This K01 award will expand my research skills in ubiquitin proteomics and skeletal muscle atrophy. These tools may enable the identification of new therapeutic strategies for alleviating the detrimental impact of skeletal muscle wasting.

项目概要/摘要 骨骼肌萎缩是许多慢性疾病和病症的结果，例如 肌肉萎缩过程的核心是蛋白质降解，其中 E3 泛素负责降解。 连接酶对于靶向特定蛋白质进行降解至关重要。许多 E3 泛素的作用。 肌肉中的连接酶尚不清楚，具体底物尚未确定。 该提案的目的是确定 E3 连接酶、F-box 和富含亮氨酸的重复蛋白 22 的作用 （Fbxl22），在骨骼肌萎缩中我的中心假设是 Fbxl22 对于骨骼肌萎缩是必需的。 强有力的初步数据支持这一假设：1) Fbxl22 mRNA。 在神经源性肌肉萎缩模型中 3 天后诱导表达，2) 过表达 我认为后肢肌肉中的 Fbxl22 会导致总泛素化蛋白显着升高。 两个具体目标： 具体目标 1：确定 Fbxl22 同工型在骨骼肌萎缩中的功能作用， 我将通过 Fbxl22 基因的敲低和过度表达来实现这一目标。 神经源性肌肉萎缩模型中的亚型并评估急性肌肉萎缩症中 Fbxl22 亚型的表达 肌肉损伤模型。 具体目标 2：确定 Fbxl22 依赖性泛素化的底物靶标。 使用 Fbxl22 基因操作与蛋白质组学分析相结合的方法来实现这一目标 以及 Fbxl22 依赖性底物上泛素基序的翻译后修饰鉴定。 随着这项提案的完成，我希望能够确定新的机制 通过识别骨骼肌的机制来了解肌肉萎缩的过程。 萎缩，我们可以探索新的治疗策略来改善个人的生活质量 存在肌肉萎缩。 爱荷华大学对其学员和初级教师提供支持的历史由来已久。 我的导师博丁教授是骨骼肌生理学、萎缩和肥大领域的世界领先者。 这个 K01 奖将扩展我在泛素蛋白质组学和骨骼肌萎缩方面的研究技能。 这些工具可能有助于确定新的治疗策略以减轻痛苦 骨骼肌萎缩的影响。