Development of a high precision and high order DNA counting platform

高精度、高阶DNA计数平台的开发

• 批准号: 10603953
• 负责人: Eleen Yee Lam Shum
• 金额: $ 108.07万
• 依托单位: ENUMERIX, INC.
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10603953
• 关键词: 3-Dimensional; Architecture; COVID-19 test; Centrifugation; Chemistry; Coupled; DNA; Data; Deposition; Detection; Development; Device or Instrument Development; Emulsions; Gene Expression Profiling; Goals; Industry; Laboratories; Light; Measurement; Methods; Molecular Biology; Mutation Detection; Noise; Oligonucleotides; Optics; Phase; Positioning Attribute; Production; RNA; Reagent; Sampling; Scanning; Signal Transduction; Speed; Surveys; System; Techniques; Technology; Testing; Tube; Work; cost; cost effective; design; digital; imager; improved; instrument; liquid biopsy; manufacturability; molecular diagnostics; multiplex detection; next generation; next generation sequencing; prototype; rapid technique; software development; stability testing; tool; usability; web-based tool

PROJECT SUMMARY/ABSTRACT Enumerix has developed a cost-effective method to perform high order DNA counting at high precision called direct count PCR (dcPCR). dcPCR uses a spin column method that generates millions of partitions directly into PCR tubes for target amplification, using standard laboratory centrifuge and thermal cyclers, followed by an ultra-rapid method to count up to 1 million copies of DNA directly from the same PCR tube. We have demonstrated that our technology far exceeds the precision of digital PCR, and can perform at a DNA counting range akin to quantitative PCR and next generation sequencing, which makes it a suitable next-generation DNA counting platform. For this proposed Phase II project we plan to further develop our dcPCR platform with the goal to distribute the technology to end-users to replace quantitative PCR and digital PCR.

项目概要/摘要 Enumerix 开发了一种经济有效的方法，可以高精度执行高阶 DNA 计数 称为直接计数 PCR (dcPCR)。 dcPCR 使用旋转柱方法，可生成数百万个 使用标准实验室离心机直接分配到 PCR 管中进行目标扩增 热循环仪，然后采用超快速方法直接从细胞中计数多达 100 万个 DNA 拷贝 同一个PCR管。我们已经证明我们的技术远远超过数字化的精度 PCR，并且可以在类似于定量 PCR 和下一代的 DNA 计数范围内进行 测序，这使其成为合适的下一代 DNA 计数平台。对于这个拟议的阶段 II 项目我们计划进一步开发我们的 dcPCR 平台，目标是将该技术分发给 最终用户取代定量 PCR 和数字 PCR。