METHODS FOR MEASURING PH IN VIVO

体内 PH 值测量方法

• 批准号: 6238339
• 负责人: DARRYL T HAMAMOTO
• 金额: $ 3.78万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-01 至 1998-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6238339
• 关键词: Primates; acidity /alkalinity; capsaicin; hyperalgesia; ischemia; laboratory rat; microelectrodes; oral facial pain; pain; sensory receptors; spinal cord

A significant proportion of patients presenting to the TMJ & Craniofacial Pain Clinic at the University of Minnesota complain of orofacial pain or headaches that can be precipitated, aggravated, or modified by palpation of localized areas over the masticatory muscles. The etiology of this phenomenon is unknown. Pathophysiological processes occuring within the masticatory muscles may be contributing to these symptoms by activating and/or sensitizing muscle nociceptors. Steen et al. ( 1992) showed that changes in pH activate cutaneous nociceptors. Bengtsson et al. (1986) reported biochemical changes, suggestive of hypoxia, in biopsy specimens taken from tender muscle. Therefore, localized areas of hypoxia may lead to decreased pH which may activate and/or sensitize muscle nociceptors leading to myalgia. My long-term goal is to gain an understanding of the anatomical pathways and physiological processes involved in muscle nociception and to apply this knowledge to elucidating the etiologic processes underlying these myalgias. I have not started a dissertation research project but I am participating in a research project in which the long-term goal is to develop methods for measuring pH in vivo. We use an active microelectrode, a KCI reference electrode with a buffer bridge, and a high impedance amplifier connected to a computer acquisition system to measure and record pH in rat hindlimb muscle. In previous experiments, we demonstrated that these microelectrodes can be used to reliably and validly measure the pH of physiological buffer solutions (Gunn et al. 1993). Currently, we are investigating the reliability and validity of our pH measurement system in muscle. Once these measurements are accomplished we will monitor muscle pH changes in response to various ischemic conditions while electrophysiologically recording the activity of nociceptive muscle afferent units . These experiments will test the hypothesis that decreased muscle pH can activate and sensitize muscle nociceptors.

很大一部分患者就诊 明尼苏达大学颞下颌关节和颅面疼痛诊所抱怨 口面部疼痛或头痛可能会突然、加剧或 通过触诊咀嚼肌局部区域进行修改。 这种现象的病因尚不清楚。 病理生理过程 发生在咀嚼肌内可能会导致这些 通过激活和/或敏化肌肉伤害感受器来缓解症状。 斯蒂恩等人 等人。 (1992) 表明 pH 值的变化会激活皮肤伤害感受器。 本特森等人。 （1986）报告了生化变化，暗示 缺氧，来自嫩肌的活检标本。 所以， 局部缺氧可能导致 pH 值降低，从而激活 和/或使肌肉伤害感受器敏感，导致肌痛。 我的长期目标 是为了了解解剖学途径和生理学 涉及肌肉伤害感受的过程并将这些知识应用于 阐明这些肌痛的病因过程。 我还没有开始论文研究项目，但我正在参与 在一个长期目标是开发方法的研究项目中 用于测量体内pH值。 我们使用活性微电极 KCI 带缓冲电桥的参比电极和高阻抗放大器 连接到计算机采集系统来测量和记录大鼠的 pH 值 后肢肌肉。 在之前的实验中，我们证明了这些 微电极可用于可靠、有效地测量pH 生理缓冲溶液（Gunn 等人，1993）。 目前，我们正在 研究我们的 pH 测量系统的可靠性和有效性 肌肉。 一旦完成这些测量，我们将监测肌肉 pH值随各种缺血条件而变化，同时 电生理学记录伤害性肌肉的活动 传入单位。 这些实验将检验以下假设： 肌肉 pH 值降低可以激活肌肉伤害感受器并使其敏感。