CHARACTERIZE FROG CUTANEOUS RECEPTORS

青蛙皮肤受体的特征

• 批准号: 6336475
• 负责人: DARRYL T HAMAMOTO
• 金额: $ 1.84万
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6336475
• 关键词: CHARACTERIZE; FROG; CUTANEOUS; RECEPTORS

Tissue acidosis may contribute to pain associated with tissue injury or inflammation. Studies have demonstrated that application of acidic solutions to human skin produced graded tissue acidosis and sustained burning pain. Recently, the frog has been proposed as an alternative animal model for pain. A drop of acetic acid (AA) applied to hindlimb skin results in nociceptive behaviors, including a quick wiping of the exposed skin. However, whether the nociceptive behaviors are evoked by decreased tissue pH produced by acetic acid is unknown. Therefore, the first specific aim of these experiments is to determine if the acidity of acetic acid (or another characteristics such as osmolarity) is important for evoking the nociceptive behaviors. Additionally, little is known about the cutaneous receptors in frogs that respond to AA. Thus, the second specific aim of these experiments is to characterize frog cutaneous receptors, particularly those responsive to AA. Our long term goal is to elucid ate the role of tissue acidosis in painful conditions. Such study may suggest novel therapeutic approaches for pain associated with tissue injury or inflammation. To complete the first specific aim, parallel behavioral and subepithelial pH measurement experiments will be performed to determine which characteristics of acetic acid are important for evoking the nociceptive behaviors. To complete the second specific aim, extracellular electrophysiological recordings will be made from the dorsal roots and spinal nerves innervating the skin of the hindlimb. The receptive fields of these primary afferents will be characterized by their conduction velocities and their responses to mechanical, themal, and chemical stimuli. The responses to acetic acid will.be compared to the results from the behavioral and subepithelial pH measurement experiments. These comparisons should reveal which classes of cutaneous receptors likely underlie the wiping response in this model of nociception. By comparing the characteristics of frog receptors responsive to AA with those of acid-sensitive cutaneous receptors reported in mammalian skin, the validity of the frog model of acidotic pain can be determined. Key Words: tissue acidosis, nociceptors, primary afferents, electrophysiology, pH measurements, frogs

组织酸中毒可能会导致与组织损伤或相关的疼痛 炎。 研究表明，应用酸性 解决人体皮肤产生的分级组织酸中毒和持续性 灼痛。 最近，青蛙被提议作为替代品 疼痛动物模型。 将一滴醋酸 (AA) 涂在后肢上 皮肤会导致伤害性行为，包括快速擦拭皮肤 暴露的皮肤。 然而，伤害性行为是否是由 乙酸引起的组织 pH 值降低尚不清楚。 因此， 这些实验的第一个具体目的是确定酸度是否 乙酸（或其他特征，例如渗透压）是 对于引发伤害性行为很重要。 此外，很少 众所周知，青蛙的皮肤受体对 AA 有反应。 因此，这些实验的第二个具体目标是表征 青蛙皮肤受体，特别是对 AA 有反应的受体。 我们的长 长期目标是阐明组织酸中毒在疼痛中的作用 状况。 此类研究可能会提出新的治疗方法 与组织损伤或炎症相关的疼痛。 为了完成第一个具体目标，并行的行为和 将进行上皮下 pH 测量实验以确定 乙酸的哪些特性对于引起 伤害性行为。 为了完成第二个具体目标， 细胞外电生理记录将由 支配后肢皮肤的背根和脊神经。 这些初级传入神经的感受野的特征是 它们的传导速度及其对机械、热、 和化学刺激。 对乙酸的反应将与 行为和上皮下 pH 测量结果 实验。 这些比较应该揭示哪些类别的皮肤 受体可能是该模型中擦拭反应的基础 伤害感受。 通过比较青蛙受体对 AA 的反应特征 哺乳动物皮肤中报道的酸敏感皮肤受体的那些， 可以确定酸中毒疼痛青蛙模型的有效性。 关键词：组织酸中毒，伤害感受器，初级传入神经， 电生理学、pH 测量、青蛙