METHODS FOR MEASURING PH IN VIVO

体内 PH 值测量方法

• 批准号: 5210053
• 负责人: DARRYL T HAMAMOTO
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5210053
• 关键词: Primates; acidity /alkalinity; capsaicin; hyperalgesia; ischemia; laboratory rat; microelectrodes; oral facial pain; pain; sensory receptors; spinal cord

A significant proportion of patients presenting to the TMJ & Craniofacial Pain Clinic at the University of Minnesota complain of orofacial pain or headaches that can be precipitated, aggravated, or modified by palpation of localized areas over the masticatory muscles. The etiology of this phenomenon is unknown. Pathophysiological processes occuring within the masticatory muscles may be contributing to these symptoms by activating and/or sensitizing muscle nociceptors. Steen et al. ( 1992) showed that changes in pH activate cutaneous nociceptors. Bengtsson et al. (1986) reported biochemical changes, suggestive of hypoxia, in biopsy specimens taken from tender muscle. Therefore, localized areas of hypoxia may lead to decreased pH which may activate and/or sensitize muscle nociceptors leading to myalgia. My long-term goal is to gain an understanding of the anatomical pathways and physiological processes involved in muscle nociception and to apply this knowledge to elucidating the etiologic processes underlying these myalgias. I have not started a dissertation research project but I am participating in a research project in which the long-term goal is to develop methods for measuring pH in vivo. We use an active microelectrode, a KCI reference electrode with a buffer bridge, and a high impedance amplifier connected to a computer acquisition system to measure and record pH in rat hindlimb muscle. In previous experiments, we demonstrated that these microelectrodes can be used to reliably and validly measure the pH of physiological buffer solutions (Gunn et al. 1993). Currently, we are investigating the reliability and validity of our pH measurement system in muscle. Once these measurements are accomplished we will monitor muscle pH changes in response to various ischemic conditions while electrophysiologically recording the activity of nociceptive muscle afferent units . These experiments will test the hypothesis that decreased muscle pH can activate and sensitize muscle nociceptors.

出现在 明尼苏达大学的TMJ＆颅面疼痛诊所抱怨 可以沉淀，加重或 通过触诊咀嚼肌肉上的局部区域进行修饰。 这种现象的病因尚不清楚。 病理生理过程 在咀嚼肌内发生可能有助于这些 通过激活和/或致敏的肌肉伤害感受器来症状。 Steen等 al。 （1992年）表明，pH的变化激活皮肤伤害感受器。 Bengtsson等。 （1986）报道了生化变化，暗示 缺氧，在从嫩肌肉中取出的活检标本中。 所以， 缺氧的局部区域可能导致pH下降，这可能会激活 和/或使导致肌痛的肌肉伤害感受器敏感。 我的长期目标 是为了了解解剖途径和生理 肌肉伤害感受的过程，并将这些知识应用于 阐明这些肌痛基础的病因学过程。 我尚未启动论文研究项目，但我正在参加 在一个研究项目中，长期目标是开发方法 用于测量体内pH。 我们使用活性微电极，一个KCI 带有缓冲桥的参考电极和高阻抗放大器 连接到计算机采集系统以测量和记录大鼠的pH 后肢肌肉。 在以前的实验中，我们证明了这些 微电极可用于可靠，有效地测量 生理缓冲溶液（Gunn等，1993）。 目前，我们是 调查我们的pH测量系统的可靠性和有效性 肌肉。 完成这些测量后，我们将监测肌肉 pH响应于各种缺血状况的变化，而 电生理记录伤害性肌肉的活性 传入单位。 这些实验将检验以下假设 肌肉pH值减少会激活并使肌肉伤害感受器敏感。