GENICULOCORTICAL PROJECTS DEVELOPMENT AND CORTICAL NEURON DIFFERENTIATION

生殖皮质项目开发和皮质神经元分化

• 批准号: 6243691
• 负责人: RICHARD T ROBERTSON
• 金额: $ 0.11万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-07-01 至 1998-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6243691
• 关键词: acetylcholinesterase; axon; brain mapping; cell death; cell differentiation; cell population study; cerebral cortex; confocal scanning microscopy; developmental neurobiology; gamma aminobutyrate; glutamates; histochemistry /cytochemistry; immunocytochemistry; laboratory rat; lateral geniculate body; neuroanatomy; neuropeptide Y; neurotransmitter metabolism; thalamus; tissue /cell culture; visual cortex

The long range goal of this project is to gain an understanding of how growing thalamocortical axons reach their targets in cortex and how the development of these thalamocortical axons affects the differentiation of cortical neurons. this project will use morphological, immunocytochemical, and laser confocal microscopic techniques to study development of geniculocortical connections in visual cortex of rats. This proposal has three major components: The first component is a morphological analysis of developing geniculocortical axons, with particular emphases on the morphology of geniculocortical axons as they grow into the cortical subplate region and as they grow from the subplate to cortical layer IV. The second component is an analysis of the morphological and neurochemical development of neurons in the subplate region and in layer IV. Both sets of experiments will be undertaken with normal and with enucleated developing rats. The third component will be an analysis of morphological development of thalamocortical projections and morphological and neurochemical development of cortical neurons maintained in organotypic tissue slice cultures. These experiments will reveal morphological and neurochemical cues important for our understanding of mechanisms by which growing thalamocortical axons can identify their target cells and how these normal developmental processes may be disturbed by early trauma-induced deafferentation.

该项目的远距离目标是了解 生长的丘脑皮质轴突在​​皮质中达到目标 这些丘脑皮质轴突的开发会影响 皮质神经元。 该项目将使用形态学，免疫细胞化学， 和激光共聚焦显微镜技术研究的发展 大鼠视觉皮层中的基因皮层连接。 该提议有 三个主要组成部分：第一个组成部分是对 开发遗传皮层轴突，特别强调 基因轴突生长成皮质的形态学 亚板区域以及它们从子板生长到皮质层IV。 第二部分是对形态和神经化学的分析 在子板区域和第四层中的神经元的发展。 两组 实验将以正常和凝聚的方式进行 发育的老鼠。 第三部分将是对形态学的分析 丘脑皮质预测和形态学的发展 在器官型中维持皮质神经元的神经化学发育 组织切片培养。 这些实验将揭示形态学和 神经化学提示对于我们对机制的理解很重要 生长的丘脑皮质轴突可以识别其靶细胞以及如何识别 正常的发育过程可能会因早期创伤引起的困扰 停用。