DEVELOPMENT OF CEREBRAL CORTEX

大脑皮层的发育

• 批准号: 2268182
• 负责人: RICHARD T ROBERTSON
• 金额: $ 82.48万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-07-01 至 1997-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2268182
• 关键词: brain mapping; cerebral cortex; developmental neurobiology

This Program Project is directed at understanding mechanisms of development of the cerebral cortex. Particular attention is focused on understanding the process of ingrowth of thalamocortical afferents and the role that these developing afferents play in the differentiation of cortex. Five individual projects bring a variety of complementary modern experimental approaches to address these issues of cortical development, using sensory cortex of rodents as a model system. Studies involve the use of: 1) axonal tracing techniques with light, electron and laser confocal microscopy to study morphological development of thalamocortical afferents [Projects 1, 2, 3]; 2) whole cell current and voltage clamping, and histochemical measures of metabolic activity to study physiological development of thalamocortical systems [Projects 2 and 3]; 3) intracellular dye injections, in situ hybridization, immunocytochemistry, and electron microscopy to study morphological development of cortical neurons [Projects 1, 2, 3, 4 and 5]; and 4) receptor binding assays, in situ hybridization, immunocytochemistry and whole cell clamping to study functional development of cortical neurons [Projects 2, 3, 4 and 5]. The use of laser confocal microscopy in a core facility will allow the relationships between developing afferent axons and differentiating cortical neurons to be studied in detail. this program is designed to maximize the complementary nature of these studies so that results from morphological, physiological, pharmacological, and molecular biological studies can be integrated to provide a comprehensive understanding of the mechanisms of development of the cerebral cortex. Such an understanding of fundamental mechanisms regulating development of the cerebral cortex is vital for determining the causes of developmental neurological disorders affecting the human cerebral cortex, and for planning strategies for therapeutic and preventative interventions.

该计划项目旨在理解发展机制 大脑皮层的。 特别关注的重点是理解 丘脑皮质传入的生长过程和作用 这些发育中的传入在皮质的区分中发挥作用。 五 单个项目带来各种互补的现代实验 使用感官来解决这些皮质发育问题的方法 啮齿动物的皮层作为模型系统。 研究涉及：1） 带有光，电子和激光共焦的轴突跟踪技术 研究丘脑皮质传入的形态发展的显微镜 [项目1，2，3]； 2）整个电池电流和电压夹紧，以及 用于研究生理的代谢活性的组织化学测量 丘脑皮质系统的开发[项目2和3]； 3）细胞内 染料注射，原位杂交，免疫细胞化学和电子 研究皮质神经元形态发展的显微镜[项目 1、2、3、4和5]; 4）原位杂交受体结合测定， 免疫细胞化学和全细胞夹紧以研究功能开发 皮质神经元[项目2、3、4和5]。 使用激光共享 核心设施中的显微镜将允许 发展传入轴突和区分皮质神经元 详细研究。 该程序旨在最大化互补 这些研究的性质，使形态，生理学， 药理学和分子生物学研究可以整合到 提供对发展机制的全面理解 大脑皮层。 对基本机制的理解 调节大脑皮层的发展对于确定 发育神经系统疾病的原因影响人脑 皮质，以及针对治疗和预防性的计划策略 干预措施。