Optoretinography: All-optical measures of functional activity in the human retina

视网膜检光术：人类视网膜功能活动的全光学测量

• 批准号: 10869100
• 负责人: DANIEL V PALANKER
• 金额: $ 16.12万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-30 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10869100
• 关键词: Acceleration; Administrative Supplement; Area; Biological Markers; Biomedical Engineering; Clinical; Clinical Research; Collaborations; Compensation; Cone; Consent; Databases; Dedications; Densitometry; Development; Diagnosis; Disease; Elements; Eye; Friends; Generations; Genotype; Goals; Human; Image; In Situ; Individual; Inherited; Interferometry; Light; Maintenance; Measurement; Measures; Methods; Molecular; Monitor; Motion; Natural regeneration; Optics; Patient Recruitments; Patients; Persons; Phase; Phenotype; Photosensitivity; Phototransduction; Pigments; Positioning Attribute; Protocols documentation; Rattus; Research; Research Infrastructure; Resolution; Responsible Person; Retina; Retinal Diseases; Retinitis Pigmentosa; Rodent; Schedule; Signal Transduction; Standardization; System; Technology; Testing; Therapeutic Intervention; Time; Tissues; Treatment Efficacy; Universities; Variant; Visual System; adaptive optics; arm; clinical translation; human subject; improved; in vivo; neural; neuron regeneration; parent grant; payment; recruit; response; retinal neuron; synergism; therapeutic evaluation; tool; visual processing; visual tracking

Project Summary/Abstract The optoretinogram (ORG) holds great promise as a probe of cone function in normal eyes, in the assessment of disease states of cones in disease, and in the evaluation of therapeutic interventions. To achieve its promise, the cellular and molecular basis of the ORG needs to be established, and methods standardized for application in the clinical setting. This supplement will pursue research opportunities that have opened up under the current Bioengineering research partnership, but were previously unforeseen. These include the addition of adaptive optics to rodent optoretinography in order to improve signal fidelity and resolution at Stanford University. Second, in a collaborative effort between UC Davis and UW, a key discrepancy was discovered previously in all extant measures of pigment bleaching; this supplement will aim to resolve this issue and elucidate the mechanistic basis of the cone ORG. Lastly, the rapid development of a patient-friendly variant of the ORG at UW has accelerated the recruitment of patients with inherited retinal disease, and created an opportunity to standardize the ORG protocols for studying genotype-phenotype correlations in a clinical setting. Support from the supplement will provide an avenue to explore these three unanticipated, exciting and critical areas, thus providing synergy to the clinical, technological and mechanistic facets of the project.

项目概要/摘要 在评估中，视视网膜图（ORG）作为正常眼睛视锥细胞功能的探针具有很大的前景 疾病中视锥细胞的疾病状态，以及治疗干预的评估。为实现其 承诺，需要建立 ORG 的细胞和分子基础，并标准化方法 在临床环境中的应用。本增刊将寻求已经开放的研究机会 在当前的生物工程研究合作伙伴关系下，但之前是不可预见的。这些包括 在啮齿动物视视网膜成像中添加自适应光学器件，以提高信号保真度和分辨率 斯坦福大学。其次，在加州大学戴维斯分校和威斯康星大学之间的合作中，一个关键的差异是 以前在所有现有的颜料漂白措施中都发现过；本补充旨在解决这个问题 问题并阐明了锥体 ORG 的机制基础。最后，患者友好型医疗技术的快速发展 华盛顿大学 ORG 的变体加速了遗传性视网膜疾病患者的招募，并且 创造了一个机会来标准化 ORG 协议，以研究基因型-表型相关性 临床环境。增刊的支持将为探索这三个意想不到的领域提供途径， 令人兴奋和关键的领域，从而为临床、技术和机械方面提供协同作用 项目。

项目成果

Optoretinography is coming of age.

视网膜检光术正在成熟。

• DOI: 10.1073/pnas.2119737118
• 发表时间: 2021
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Roorda,Austin