Monitoring Pesticide Exposure and Accumulation: An Improved Rapid Identifier for all Pesticide Classes

监测农药暴露和累积：改进的所有农药类别的快速识别器

• 批准号: 9447172
• 负责人: KEVIN M SPENCER
• 金额: $ 49.27万
• 依托单位: EIC LABORATORIES, INC.
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-03-15 至 2020-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9447172
• 关键词: Adsorption; Atrazine; Automation; Biological; Biology; Brazil; Calibration; China; Chlorinated Hydrocarbons; Collection; Communities; Complex; Computer software; Coupled; Data; Data Set; Detection; Devices; Elements; Environmental Exposure; Environmental Monitoring; Environmental Pollution; Epidemiology; Evaluation; Exposure to; Failure; Film; Floor; Glycine; Goals; Gossypium; Health; Hour; House Dust; Human; Humidity; Ingestion; Laboratories; Liquid substance; Locales; Measurement; Measures; Methods; Monitor; National Institute of Environmental Health Sciences; North Carolina; Organophosphates; Outcome; Parathion; Pesticide Residues; Pesticides; Phase; Process; Protocols documentation; Raman Spectrum Analysis; Receiver Operating Characteristics; Reporting; Reproducibility; Resolution; Sampling; Signal Transduction; Simazine; Small Business Innovation Research Grant; Specific qualifier value; Spectrum Analysis; Surface; Surveys; System; Techniques; Technology; Temperature; Testing; Thinness; Time; Training; Triazines; United States National Institutes of Health; Universities; Urinalysis; Urine; Validation; Work; blind; cost; design; dosimetry; epidemiology study; farm worker; field study; high risk population; improved; innovation; instrument; novel; organochlorine pesticide; pesticide exposure; planetary Atmosphere; pollutant; portability; programs; sensor; tool; vapor; vibration; volunteer; water sampling; water vapor

Project Summary The proposed program aims to quantify farmworker exposure to pesticides using Surface Enhanced Raman Spectroscopy (SERS) and an inexpensive sensor which can also be used for urinalysis. An earlier program, supported by NIEHS, provided reliable results for organophosphate (OP) and organochlorine (OC) pesticides. Fifty OP and OC pesticides and metabolites were evaluated in the laboratory in the low ppb range, and acephate and methyl parathion were detected at farmworker camps. In this program, we will utilize an innovative SERS sensing element which can also detect triazine pesticides, such as atrazine, simazine and cyromazine. In Phase I we developed a glycine-coated sensor that stabilized the adsorbed triazine ring leading to significant signal enhancement. This novel sensor detects triazines down to 1 ppb, is stable at temperatures and humidities encountered by farmworkers, and can be used for urinalyses. A new, combined sensor, to be developed in Phase II, will measure over 75 pesticides and/or metabolites at a small fraction of the cost of current analytical laboratory methods. It will provide the NIH (particularly the NIEHS Exposure Biology program) the means to develop a large data set on daily exposure/ingestion of pesticides. This approach should allow epidemiological studies and predictions of long-term health outcomes. Core technology has been demonstrated. The Phase II program is designed to demonstrate the capabilities of a combined sensor and provide full analytical specifications, finalize protocols for dosimetry, urine, cotton wipe and house dust collection measurements. Precision and detection limits for pesticides/metabolites of relevance and a thorough interference analysis will be quantitatively defined. System blind tests, statistical software optimization using training sets and unknown verifications and field testing with volunteer North Carolina farmworkers will be performed. Spectral dosimetry and urinalysis results will be correlated to worker activity through worker surveys. An automated system for third party evaluation will be completed in Phase II.

项目概要 拟议的计划旨在使用表面增强拉曼来量化农场工人接触农药的情况 光谱学 (SERS) 和一种也可用于尿液分析的廉价传感器。之前的一个节目， 在 NIEHS 的支持下，为有机磷 (OP) 和有机氯 (OC) 农药提供了可靠的结果。 在实验室中对 50 种低 ppb 范围内的 OP 和 OC 农药及代谢物进行了评估，并且 在农场工人营地检测到乙酰甲胺磷和甲基对硫磷。在这个程序中，我们将利用一个 创新的SERS传感元件，还可以检测三嗪类农药，如莠去津、西玛津和 灭蝇胺。在第一阶段，我们开发了一种甘氨酸涂层传感器，可以稳定吸附的三嗪环 导致信号显着增强。这种新型传感器可检测低至 1 ppb 的三嗪，在 农场工人遇到的温度和湿度，可用于尿液分析。一个新的、组合的 将在第二阶段开发的传感器将测量超过 75 种农药和/或代谢物，而只需一小部分 当前分析实验室方法的成本。它将提供 NIH（特别是 NIEHS Exposure） 生物学计划）是开发有关每日接触/摄入农药的大型数据集的方法。这 方法应允许流行病学研究和长期健康结果预测。 核心技术已得到论证。第二阶段计划旨在展示 组合传感器并提供完整的分析规格，最终确定剂量测定、尿液、棉擦拭的方案 和房屋集尘测量。农药/代谢物的精密度和检测限 将定量定义相关性和彻底的干扰分析。系统盲测、统计 使用训练集和未知验证以及志愿者 North 的现场测试进行软件优化 卡罗莱纳州农场工人将进行表演。光谱剂量测定和尿液分析结果将与工人相关 通过工人调查开展活动。第三方评估自动化系统将在第二阶段完成。