Monitoring Pesticide Exposure and Accumulation: An improved Rapid Identifier for all Pesticide Classes

监测农药暴露和积累：针对所有农药类别的改进快速识别器

基本信息

批准号：
8903333
负责人：
KEVIN M SPENCER
金额：
$ 14.99万
依托单位：
EIC LABORATORIES, INC.
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-03-15 至 2015-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8903333
关键词：
4-nitrophenol Adsorption Amines Atrazine Automation Azides Biological Biology Body Fluids Calibration Camping Chemicals Chlorinated Hydrocarbons Communities Complex Coupled Data Data Set Databases Detection Diesel Fuels Electrons Elements Environmental Monitoring Environmental Pollution Epidemiologic Studies Exposure to Film Food Supply Goals Health Human Humidity Hydrogen Bonding Ingestion Laboratories Least-Squares Analysis Life Liquid substance Measures Methods Methyl Parathion Monitor National Institute of Environmental Health Sciences Organophosphates Outcome Pesticide Residues Pesticides Phase Population Populations at Risk Process Protocols documentation Raman Spectrum Analysis Reading Reporting Reproducibility Research Resolution Sampling Signal Transduction Silver Simazine Small Business Innovation Research Grant Solutions Source Spectrum Analysis Statistical Algorithm Sunlight Surface Techniques Technology Temperature Testing Time Triazines United States National Institutes of Health Urinalysis Urine Work cost cost effective design electron density farm worker high risk improved innovation interest novel organochlorine pesticide pesticide exposure planetary Atmosphere programs public health relevance sensor surface coating tool ultraviolet irradiation vapor

项目摘要

DESCRIPTION (provided by applicant): An inexpensive disposable vapor sensor, which can also be used for urinalysis, can provide rapid field monitoring of pesticide exposure. The sensor utilizes Surface-Enhanced Raman Spectroscopy (SERS) to detect chemicals, like pesticides, that adsorb strongly on roughened SERS substrates in the high parts-pre- trillion range. As the sensors are tuned to the analytes of interest, interferences from more concentrated chemicals are limited. An earlier research program provided reliable results for organophosphate (OP) and organochlorine (OC) pesticides. In that program, 50 OP and OC pesticides or metabolites were evaluated in the laboratory at the low ppb range, with acephate and methyl parathion detection at farmworker camps. In this program, we will fabricate an innovative SERS sensing element for the detection of triazine pesticides, such as atrazine, simazine and cyromazine. Sensors will be optimized by an azide loading to improve the electron density and yield strong hydrogen bonding with the triazine amine groups. This novel sensor is expected to detect triazines in concentrations of 1 ppb or less and withstand the temperature and humidity conditions encountered by farmworkers. The new sensor can be easily combined with our previous sensor to measure >75 pesticides or metabolites at a fraction of the cost of current analytical laboratory methods, thus providing the NIH (particularly the NIEHS Exposure Biology program) with a large data set on the daily exposure/ingestion of pesticides. This data will allow epidemiological studies and predictions of long-term health outcomes. Core technology concepts have been demonstrated. A previous program demonstrated organochlorine and organophosphate pesticides can be detected down as low as 100 ppt. EIC Laboratories has demonstrated in preliminary studies that an azide-coated SERS sensor is several orders of magnitude more sensitive to triazines than previous sensors. The Phase I program is designed to demonstrate detection of triazine pesticides and metabolites at the 1 ppb level, show an extended field lifetime and an increased sensitivity for direct readings of triazine pesticide metabolites.

描述（由申请人提供）：一种廉价的一次性蒸汽传感器，也可用于尿液分析，可以提供农药暴露的快速现场监测。该传感器利用表面增强拉曼光谱（SERS）来检测吸附的化学物质，例如农药。由于传感器针对感兴趣的分析物进行了调整，因此早期的研究项目提供了可靠的结果。在该计划中，我们将在实验室中对 50 种 OP 和 OC 农药或代谢物进行低 ppb 范围的评估，并在农场工人营地进行乙酰甲胺磷和甲基对硫磷的检测。用于检测三嗪农药（例如莠去津、西玛嗪和灭蝇胺）的创新 SERS 传感元件将通过叠氮化物负载进行优化。提高电子密度并与三嗪胺基团产生强氢键，这种新型传感器有望检测浓度为 1 ppb 或更低的三嗪，并能承受农场工人遇到的温度和湿度条件。我们之前的传感器可测量 >75 种农药或代谢物，而成本只是当前分析实验室的一小部分方法，从而为 NIH（特别是 NIEHS 暴露生物学项目）提供有关每日接触/摄入农药的大量数据。这些数据将有助于流行病学研究和长期健康结果的预测。先前的项目表明，有机氯和有机磷农药的检测浓度可低至 100 ppt。EIC 实验室已在初步研究中证明，叠氮化物涂层的 SERS 传感器可以检测到低至 100 ppt 的有机氯和有机磷农药。第一阶段项目旨在展示 1 ppb 水平的三嗪农药和代谢物的检测，显示出更长的现场寿命和更高的直接读数三嗪农药代谢物的灵敏度。