REGULATION OF PROTEOLYSIS IN PREMATURE NEONATES

早产新生儿蛋白水解的调节

基本信息

批准号：
2392322
负责人：
Brenda Poindexter
金额：
$ 0.77万
依托单位：
INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-04-01 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/2392322
关键词：
clinical research cysteine developmental nutrition dietary aminoacid dietary supplements essential aminoacid human subject insulin leucine nutrition related tag parenteral feedings phenylalanine premature infant human proteolysis stable isotope tyrosine

项目摘要

The overall objective of this proposal is to further define the regulation of proteolysis in premature neonates. As the balance between proteolysis and protein synthesis ultimately determines whether protein accretion and growth will occur, identifying potential regulators of proteolysis may serve as a means to maximize protein accretion in this nutritionally vulnerable population. Exogenous insulin is currently being used in extremely premature neonates to promote growth, although the effect of insulin on protein metabolism is unknown. In addition, parenteral nutrition solutions are frequently used in sick and premature neonates who are unable to tolerate enteral feeding. The quantity and quality of amino acids provided to these neonates is likely to be a critical factor in maximizing protein accretion. The specific aims of this proposal are to: 1)determine how a continuous insulin infusion affects proteolysis in the extremely premature neonate 2)evaluate the extent to which graded amino acids suppress proteolysis in premature infants and 3)determine whether adding tyrosine and cysteine (thought to be essential amino acids in premature infants) to currently available amino acid solutions will further improve rates of protein accretion. Each of these specific aims will be pursued using the stable isotope tracers of the essential amino acids leucine and phenylalanine, whose endogenous rate of appearance is reflective of whole body proteolysis. Performing these studies will provide insight into the physiology of protein accretion, and thus the growth process. Ultimately, this information may serve as the basis for improved nutritional management of premature infants.

该提案的总体目标是进一步明确监管早产儿的蛋白水解作用。由于蛋白水解之间的平衡蛋白质合成最终决定蛋白质的积累和生长将会发生，确定蛋白水解的潜在调节因子可能作为一种最大限度地增加蛋白质在营养方面的手段弱势群体。目前外源性胰岛素主要用于对极早产儿有促进生长的作用，虽然胰岛素对蛋白质代谢的影响尚不清楚。此外，肠外注射营养液经常用于患病和早产新生儿无法耐受肠内喂养。氨基的数量和质量为这些新生儿提供的酸可能是一个关键因素最大限度地增加蛋白质。该提案的具体目标是： 1)确定连续胰岛素输注如何影响蛋白质水解极早产新生儿 2) 评估分级氨基的程度酸抑制早产儿的蛋白水解作用，并且 3) 确定是否添加酪氨酸和半胱氨酸（被认为是人体必需的氨基酸）早产儿）到目前可用的氨基酸溶液将进一步提高蛋白质积累率。这些具体目标中的每一个将使用必需氨基酸的稳定同位素示踪剂进行追踪亮氨酸和苯丙氨酸，其内源出现率为反映全身蛋白水解作用。进行这些研究将提供对蛋白质积累生理学的深入了解，从而成长过程。最终，这些信息可以作为改善早产儿的营养管理。