[ShEEP/LAMb] Request for a Seahorse Extracellular Flux Analyzer

[ShEEP/LAMb] 索取 Seahorse 细胞外通量分析仪

• 批准号: 9209599
• 负责人: CHRISTOPHER P CARDOZO
• 金额: --
• 依托单位: JAMES J PETERS VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-05-01 至 2016-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9209599
• 关键词: Affect; Alzheimer' s Disease; Biological Sciences; Cell Culture System; Cell Culture Techniques; Cell Line; Cell Respiration; Cell physiology; Cells; Characteristics; Charge; Chemicals; Computer software; Cultured Cells; Data Analyses; Dementia; Disease; Energy-Generating Resources; Fatty acid glycerol esters; Funding; Genus Hippocampus; Glycolysis; Gold; HIV; Health; Immune System Diseases; Incubators; Individual; Institution; Life; Liquid substance; Malignant Neoplasms; Manufacturer Name; Measurement; Measures; Medical; Metabolic; Metabolism; Mitochondria; Nature; Oxygen; Pathogenesis; Pilot Projects; Price; Process; Quality of life; Reagent; Request for Applications; Research Personnel; Site; Spinal cord injury; Time; Tissues; Training; Traumatic Brain Injury; Veterans; carbohydrate metabolism; design; disability; extracellular; instrument; medical schools; metabolomics; sensor; standard measure; sugar

The instrument requested in this application is a Seahorse Extracellular Flux Analyzer package consisting of 6 well and 96 well platforms suitable for pilot studies and larger high throughput studies, respectively. These instruments are manufactured by Seahorse Bioscience. The purchase price includes on-site training and a one-year warranty. Data analysis software is available from the manufacturer for no charge and may be installed on an unlimited number of workstations. The instrument measures cellular respiration through changes over time on oxygen concentration and pH and is able to discern the portion of cellular respiration attributable to mitochondria or glycolysis. In addition, pharmacological manipulations permit one to measure mitochondrial and glycolytic reserve capacity and baseline cellular respiration. Using additional reagents, one can measure the fraction of cellular respiration attributable to metabolism of carbohydrates and fats. These measurements are obtained using two, separate sensors that are immersed in cell culture fluid and pre-mixed reagents purchased from the manufacturer. The instrument is designed to be easily used by individuals who do not have specialized training in metabolomics and is the de-facto standard for measuring metabolic parameters in living cells. Measurements may be obtained from cultured cell lines, primary cultures or tissue sections. Specialized, disposable plastic plates must be used for the measurements. Cells or tissues are placed into wells on these plates and can be maintained in cell culture incubators similarly to routine cell culture systems. No equivalent instrument is available at JJPVA. Use of the closest Seahorse platform, located at our academic affiliate the Icahn School of Medicine at Mount Sinai, is not feasible because of the challenges of transporting cell cultures over the 10 miles that separate the institutions. If funded, this application would afford JJPVA investigators access to the gold standard for cell metabolomics measurements. The instrument will be used in VA-funded projects in the fields of Alzheimer's disease, traumatic brain injury, HIV pathogenesis, and spinal cord injury. The instrument would add the capacity for systematic study of cellular metabolism in cells and cultured cells and, thereby, substantially broaden and enhance the understanding of medical conditions that affect the health and quality of life of veterans impacted by these diseases and disabilities.

本申请中要求的仪器是 Seahorse 细胞外通量分析仪包 由 6 孔和 96 孔平台组成，适用于试点研究和更大规模的高通量研究， 分别。这些仪器由 Seahorse Bioscience 制造。购买价格 包括现场培训和一年保修。数据分析软件可从 制造商免费提供，并且可以安装在无限数量的工作站上。这 仪器通过氧浓度和 pH 值随时间的变化来测量细胞呼吸 并能够辨别细胞呼吸归因于线粒体或糖酵解的部分。在 此外，药理学操作允许测量线粒体和糖酵解储备 能力和基线细胞呼吸。使用额外的试剂，可以测量 细胞呼吸归因于碳水化合物和脂肪的代谢。这些测量值是 使用浸入细胞培养液和预混合试剂中的两个独立传感器获得 从制造商处购买。该仪器的设计是为了方便个人使用 没有接受过代谢组学方面的专门培训，是测量代谢的事实上的标准 活细胞中的参数。测量值可以从培养的细胞系、原代培养物或 组织切片。测量时必须使用专用的一次性塑料板。细胞或 将组织放入这些板上的孔中，并可以类似地保存在细胞培养箱中 常规细胞培养系统。 JJPVA 没有类似的工具。使用最近的 Seahorse 平台位于我们的学术附属机构西奈山伊坎医学院，不是 由于在 10 英里之内运输细胞培养物存在挑战，因此这是可行的 机构。如果获得资助，该应用程序将使 JJPVA 调查人员能够获得黄金标准 细胞代谢组学测量。该仪器将用于 VA 资助的项目，涉及以下领域： 阿尔茨海默病、创伤性脑损伤、艾滋病毒发病机制和脊髓损伤。仪器 将增加系统研究细胞和培养细胞中细胞代谢的能力， 从而大大拓宽和增强对影响健康的医疗状况的理解 受这些疾病和残疾影响的退伍军人的健康和生活质量。