The Resource of the MMRRC at UC Davis

加州大学戴维斯分校 MMRRC 的资源

• 批准号: 10565675
• 负责人: KC KENT LLOYD
• 金额: $ 117.02万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-30 至 2025-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10565675
• 关键词: Acceleration; Adopted; Advertising; Alleles; Analgesics; Applied Research; Archives; Area; Awareness; Bacterial Artificial Chromosomes; Biomedical Research; Biotechnology; Breeding; CRISPR/Cas technology; California; Caring; Collection; Communities; Contracts; Cost efficiency; Country; Cryopreservation; Custom; Database Management Systems; Development; Disease; Diverse Workforce; ES Cell Line; Effectiveness; Embryo; Ensure; Environment; Equipment; Ethylnitrosourea; Evaluation; Feedback; Freedom; Funding; Gene Targeting; Genomics; Genotype; Goals; Guidelines; Health; Human Resources; Individual; Informatics; Information Dissemination; Infrastructure; Injections; Institution; Intelligence; Intracytoplasmic Sperm Injections; Knock-out; Leadership; Marketing; Mentors; Mission; Modeling; Molecular Analysis; Mus; Mutant Strains Mice; National Heart, Lung, and Blood Institute; Outcome; Performance; Phenotype; Policies; Positioning Attribute; Prevention; Procedures; Protocols documentation; Published Comment; Recommendation; Reproducibility; Research; Research Activity; Research Personnel; Research Project Grants; Resource Development; Resources; Role; Scientist; Security; Services; Speed; Technology; Training; Transgenic Organisms; United States National Institutes of Health; Universities; Work; biobank; biomedical resource; blastocyst; community engaged research; congenic; embryonic stem cell; genetic variant; genome editing; gut microbiome; high standard; improved; innovation; meetings; microbiome analysis; microbiota transplantation; mouse model; multiple omics; mutant; mutant mouse model; next generation; online resource; operation; pathogen; programs; repository; tool; web site; zygote

ABSTRACT: Resource Section This proposal seeks renewal of 5 year support for the infrastructure, personnel, and resources of the Mutant Mouse Resource and Research Center (MMRRC) at the University of California (UC) Davis (MMRRC-UCD). Since its inception 20 years ago, the MMRRC-UCD has been one of the primary mutant mouse archive and distribution repositories in the world. As the largest of the 4 Centers in the MMRRC Consortium, the MMRRC- UCD has assumed a position of leadership in innovation and resource development. The MMRRC-UCD has introduced new products (e.g., KOMP knockout first alleles in ES cells, CRISPR/Cas9 genome edited alleles in zygotes) and initiated new services (e.g., blastocyst injection, ICSI, speed congenics, genetic variant modeling, microbiome analysis) that have added scientific value to mouse models submitted to the MMRRC, which in turn has enhanced utilization of the MMRRC by the scientific research community. As a result, to date the MMRRC-UCD leads the Consortium in the number of alleles archived (50,040 out of 59,847), orders (6,293 out of 13,165), and investigators served (4,372 out of 6477) at 2,445 institutions in 23 countries. The MMRRC- UCD has leveraged its infrastructure and expertise to extend its service role by contracting with categorical NIH institutes (e.g., NHLBI BayGenomics project), biotechnology companies (Genentech), and several organizations (e.g., the BaSH Consortium) to archive, distribute, custom breed, genotype, and phenotype 1000’s of transgenic and knockout, gene-targeted ES cell lines, BAC-transgenic, CRE, ENU-induced, and other mutant mouse collections. In addition, the MMRRC-UCD has worked with its MMRRC Consortium partners and NIH program staff to develop and implement advertising and marketing strategies, database management and informatics applications, website and online resources, customer and user services, operating procedures and protocols, and policies and practicies for shared governance. Further, over the last several years, the MMRRC-UCD completed several research projects in multiple areas that have enhanced operations of the resource, including in cryopreservation, gut microbiome analysis, mouse analgesics administration, genetic variant modeling using CRISPR/Cas9, and more. Over the next 5 years, the MMRRC- UCD looks forward to the assignment of another ~250 mouse lines (Type 1), providing users access to several new services including Fast-Track Genome Editing and Genomic Integration Analysis, and conducting 3 new research projects in the areas of microbiome transplantation, quantitative embryo evaluation, and multi-omic molecular analyses. By archiving and delivering research mouse products and services effectively and efficiently, and by conducting applied research activities to improve operations of the resource, the MMRRC- UCD aims to continue as a scientifically valuable biorepository for the biomedical research community. Over the next 5 years, we shall focus our efforts on optimizing the quality of our resource, ensuring its relevance to individual users, and seeking maximum performance for the benefit of all whom we serve.

摘要：资源部分 该提案寻求续签5年对突变体基础设施，人员和资源的支持 加利福尼亚大学戴维斯分校（MMRRC-UCD）的小鼠资源与研究中心（MMRRC）。 自20年前成立以来，MMRRC-UCD一直是主要的突变小鼠存档之一，并且 世界上的分销存储库。作为MMRRC财团中最大的4个中心，MMRRC- UCD在创新和资源开发方面担任领导地位。 MMRRC-UCD具有 引入了新产品（例如，ES细胞中的Komp敲除第一等位基因，CRISPR/CAS9基因组编辑了等位基因 Zygotes）并启动了新服务（例如，胚泡注射，ICSI，速度友善，遗传变异建模， 微生物组分析）为提交给MMRRC的小鼠模型增加了科学价值， 转弯已经提高了科学研究界对MMRRC的利用。结果，迄今为止 MMRRC-UCD领导财团的等位基因数量（59,847分中的50,040），订单（6,293 在13,165）中，在23个国家 /地区的2,445个机构中，调查人员在2,445个机构中服役（4,372个）。 mmrrc- UCD利用其基础架构和专业知识来通过与分类合同来扩展其服务角色 NIH Institutes（例如NHLBI Baygenomics项目），生物技术公司（Genentech）和几个 组织（例如，狂欢财团）归档，分布式，定制品种，基因型和表型 1000的转基因和敲除，基因靶向ES细胞系，BAC-转基因，CRE，ENU诱导的和 其他突变小鼠收集。此外，MMRRC-UCD已与其MMRRC联盟合作 合作伙伴和NIH计划人员制定和实施广告和营销策略，数据库 管理和信息应用程序，网站和在线资源，客户和用户服务， 操作程序和协议，共同治理的政策和实践。此外，最后 几年，MMRRC-UCD在多个领域完成了几项研究项目 资源的操作，包括冷冻保存，肠道微生物组分析，小鼠镇痛药 管理，使用CRISPR/CAS9的遗传变体建模等等。在接下来的5年中，MMRRC- UCD期待另一个〜250鼠标线（类型1）的分配，使用户访问多个 新服务，包括快速轨道基因组编辑和基因组整合分析，并进行3个新的服务 微生物组移植，定量胚胎评估和多运动的研究项目 分子分析。通过归档和交付研究鼠标产品和服务有效，以及 有效，通过开展应用研究活动以改善资源的运营，MMRRC- UCD的目标是继续作为生物医学研究界的科学有价值的生物座席。超过 在接下来的5年中，我们将重点放在优化资源质量上，以确保其与之相关 个人用户，并寻求最高绩效，以使我们服务的所有人受益。