ShEEP request for high-resolution flow cytometry system

ShEEP 请求高分辨率流式细胞术系统

• 批准号: 9796556
• 负责人: ROLANDO E RUMBAUT
• 金额: --
• 依托单位: MICHAEL E DEBAKEY VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2019-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9796556
• 关键词: 16 year old; 20 year old; Blood coagulation; Cells; Chronic lung disease; Clinical; Collaborations; Core Facility; Data; Detection; Disease; Equipment; Flow Cytometry; Fluorochrome; Fostering; Future; Generations; Goals; Hospitals; Image; Individual; Infection; Laboratories; Lasers; Lung diseases; Maintenance; Malignant Neoplasms; Medical center; Price; Production; Program Evaluation; Research; Research Personnel; Resolution; Resources; Sampling; Schedule; Scientist; Secure; Sepsis; Services; Side; Site; System; Talents; Thrombosis; Time; Training; Travel; Tube; Veterans; Viola; cost; design; detector; equipment acquisition; high resolution imaging; high throughput analysis; improved; instrument; microvesicles; nanoparticle; particle; programs; recruit; research study; transmission process

This application will support the purchase of a state-of-the-art flow cytometry system, (CytoFLEX LX Flow Cytometer Plate Loader with Mode Control), to be used as a core research resource at Michael E. DeBakey VA Medical Center. This system will enhance the research program of 7 VA investigators and enable them to obtain high-throughput, high-sensitivity flow cytometry data for research studies focused on conditions relevant to Veterans including thrombosis, lung disease, cancer, and sepsis, among others. This instrument will replace a 16 year-old flow cytometer available currently in our research building. That instrument was transferred to the Research Service Line in 2013, in lieu of being discarded, after our hospital upgraded its clinical flow cytometer. The existing flow cytometer is outdated and no longer in production, which poses a challenge for securing timely and effective maintenance, which adversely impacts our VA researchers. Although our academic affiliate has comparable flow cytometry systems in core facilities nearly 1.5 miles away, the delays associated with travel to/from the affiliate and limited scheduling availability induce obstacles in using the resources at the affiliate. Having access to this equipment on station will enhance the existing core research equipment at our facility, help promote collaborative research among our VA investigators, and provide a tremendous improvement to our overall research program. This is expected to promote further collaboration among VA investigators as well as improve our efforts to recruit and retain talented investigators to our medical center.

该应用程序将支持购买最先进的流式细胞仪 系统（带模式控制的 CytoFLEX LX 流式细胞仪板装载机） 用作迈克尔·E·德贝基退伍军人医疗中心的核心研究资源。 该系统将增强 7 名 VA 研究人员的研究计划，并使 他们获得高通量、高灵敏度的流式细胞术数据用于研究 研究重点是与退伍军人相关的疾病，包括血栓形成、肺 疾病、癌症和败血症等。该仪器将取代 16 我们的研究大楼目前有一台已有一年历史的流式细胞仪。那 仪器于 2013 年转移至研究服务热线，而不是 我院临床流式细胞仪升级后废弃。现有的 流式细胞仪已经过时，不再生产，这带来了挑战 确保及时有效的维护，这对我们的 VA 产生了不利影响 研究人员。尽管我们的学术附属机构拥有类似的流式细胞仪 核心设施中的系统距离近 1.5 英里，与旅行相关的延误 往返联营公司和有限的日程安排会导致使用障碍 附属机构的资源。在空间站上使用该设备将 增强我们设施现有的核心研究设备，帮助促进 我们的 VA 研究人员之间的合作研究，并提供了巨大的 改进我们的整体研究计划。预计此举将促进 VA 研究人员之间的进一步合作以及改进我们的努力 招募并留住有才华的研究人员到我们的医疗中心。