ShEEP Request for Super Resolution Laser Scanning Confocal Microscopy System

ShEEP 请求超分辨率激光扫描共焦显微镜系统

• 批准号: 9361137
• 负责人: ROLANDO E RUMBAUT
• 金额: --
• 依托单位: MICHAEL E DEBAKEY VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-01-01 至 2017-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9361137
• 关键词: Biological; Blood Coagulation Disorders; Blood Platelets; Blood coagulation; Caliber; Carbon Dioxide; Cell Culture Techniques; Cells; Chronic Kidney Failure; Collaborations; Commuting; Complement; Computer Hardware; Computer software; Computers; Core Facility; Cultured Cells; Diabetes Mellitus; Disease; Electron Microscopy; Equipment; Event; Face; Fluorescent Dyes; Fostering; Goals; Humidity; Hybrids; Image; Individual; Laser Scanning Confocal Microscopy; Lasers; Lead; Medical center; Microscope; Microscopy; Modeling; Operating System; Program Evaluation; Recruitment Activity; Research; Research Personnel; Resolution; Sampling; Scanning; Schedule; Scientist; Shapes; Silicone Oils; Site; Speed; Structure; System; Talents; Technology; Temperature; Time; Tissues; Training; Veterans; cellular imaging; cellular targeting; design; flasks; improved; instrument; light microscopy; microscopic imaging; nanometer; optical spectra; programs; solid state; vibration

This application will support the purchase of a state-of-the-art microscopy system, a super resolution laser scanning confocal microscope (Olympus FV3000). This system will enhance the research program of 7 VA investigators and enable them to obtain microscopy images of cellular and subcellular events with unprecedented resolution (up to 120 nanometers). There is no equipment available on our campus for this purpose. Although our academic affiliate has comparable microscopy systems in core facilities, our VA investigators face obstacles in using them due to limited availability in scheduling which often lead to significant delays, and cumbersome commute to/from our affiliate with biological samples. Having this technology available on our campus will represent a major benefit to our VA researchers, providing exceptional microscopy capabilities. For example, 4 of the users study platelets (cells with diameter of 2-3 micrometers) and typically rely on electron microscopy to study their cellular components. The super resolution system will enable these researchers to obtain microscopy images of platelet shape and structure with unprecedented capabilities. The system contains 7 lasers, which will facilitate simultaneous imaging of multiple cellular targets, including identify their interactions. Further, the system will be particularly helpful for studies on cultured cells, with its inverted microscope design with high numerical aperture silicone oil objectives and a temperature, humidity, and carbon dioxide-controlled incubation chamber. This system will complement the existing core research equipment at our facility, and will provide a tremendous enhancement to our overall research program. This is expected to promote further collaboration among VA investigators as well as improve our efforts to recruit and retain talented investigators to our medical center.

该应用程序将支持购买最先进的显微镜 系统，超分辨率激光扫描共焦显微镜（奥林巴斯FV3000）。这 系统将增强 7 名 VA 研究人员的研究计划，使他们能够 获得前所未有的细胞和亚细胞事件的显微镜图像 分辨率（高达 120 纳米）。我们的校园内没有可供使用的设备 这个目的。尽管我们的学术附属机构在以下领域拥有类似的显微镜系统 核心设施，我们的 VA 调查员在使用它们时面临着由于有限的障碍 调度的可用性往往会导致严重的延误和麻烦 携带生物样本往返于我们的附属机构。拥有这项技术 在我们的校园里将为我们的 VA 研究人员带来重大好处，提供 卓越的显微镜功能。例如，4 位用户研究血小板（细胞） 直径为2-3微米），通常依靠电子显微镜来研究 他们的细胞成分。超分辨率系统将使这些研究人员能够 获得血小板形状和结构的显微图像，这是前所未有的 能力。该系统包含 7 个激光器，这将有助于同时成像 多个细胞目标，包括识别它们的相互作用。此外，该系统将 其倒置显微镜设计特别有助于培养细胞的研究 高数值孔径硅油物镜和温度、湿度和碳 二氧化碳控制的培养室。该系统将补充现有的核心 我们设施中的研究设备，将为我们的研究提供巨大的增强 总体研究计划。预计这将促进 VA 之间的进一步合作 调查员，并加强我们招募和留住有才华的调查员的努力 我们的医疗中心。