Resource Development Core

资源开发核心

• 批准号: 10747628
• 负责人: Gerard L Apodaca
• 金额: $ 23.17万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2028-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10747628
• 关键词: 3-Dimensional; 4D Imaging; Address; Antibodies; Antigens; Architecture; Basic Science; Binding; Biological; Biological Models; Cell physiology; Cells; Collaborations; Collagen; Collection; Computers; Country; Creativeness; Cues; Custom; Dedications; Development; Disease; Educational process of instructing; Electron Microscope; Electrons; Energy Transfer; Ensure; Equipment; Faculty; Fluorochrome; Freeze Fracturing; Freeze Substitution; Functional disorder; Funding; Goals; Gold; Hand; Image; Imaging Device; Imaging technology; Incubators; Individual; Industry; Infrastructure; Injury; Kidney; Label; Light; Location; Lower urinary tract; Maintenance; Methods; Microfluidics; Microscope; Microscopy; Mission; Organ; Organelles; Organoids; Performance; Physiology; Polarization Microscopy; Positioning Attribute; Protocols documentation; Quality Control; Reagent; Recording of previous events; Renal function; Research; Research Personnel; Resource Development; Resources; Scanning; Schedule; Site; Standardization; Structure; System; Technology; Technology Transfer; Testing; Three-Dimensional Imaging; Time; Tissues; Training; Translational Research; Urinary tract; Validation; Variant; Vertebral column; Work; base; cell type; cohort; cost; design; extracellular; image processing; imaging facilities; imaging modality; imaging study; imaging system; improved; innovation; kidney cell; kidney dysfunction; kidney imaging; manufacture; mechanotransduction; microscopic imaging; multidisciplinary; novel; petabyte; repaired; response; screening; second harmonic; skills; superresolution imaging; technology development; technology platform; terabyte; tool; tool development; transmission process; two-photon; ultra high resolution; virtual reality

ABSTRACT: KIDNIT (Kidney Imaging: Developing Novel and Innovative Tools) is the Pittsburgh Center for Kidney Research’s Resource Development Core. Working in collaboration with kidney investigators and the Center’s Physiology and Model Systems Core, KIDNIT will build and adapt cutting-edge light, super-resolution, and electron microscopic imaging modalities and technologies to address open questions related to kidney function and dysfunction. The broad and long-range goals of KIDNIT are as follows: Aim 1 is to nurture a dynamic incubator space for imaging tool development. KIDNIT will capitalize on the resources provided by the Center for Biological Imaging (CBI), one of the largest imaging facilities in the country. The CBI has a dedicated expert faculty, a large cohort of research technicians, and a vast array of imaging equipment including over 40 contemporary widefield, confocal, two-photon, TIRF, light-sheet, super-resolution, and electron microscopes. Aim 2 is to develop new and custom-made imaging resources, tools, and technologies specifically to augment and advance kidney research. KIDNIT will leverage the CBI’s faculty expertise to develop, adapt, and implement new imaging tools for kidney researchers. Example projects in development include novel clearing and 3D imaging protocols to reveal and quantify kidney architecture and function at the level of the entire organ; analyzing collagen organization and structure in normal and fibrotic tissues using a recently developed variant of polarized light microscopy (instant polarized light microscopy; iPOL); development of new protocols that employ fluorescent lifetime imaging and fluorescent energy transfer to rapidly assess kidney cell function and responses to extracellular cues; use of high throughput platforms to perform large-scale screening of organoid form and function; and development of novel microfluidic chambers. Mechanisms are in place to ensure training and information transfer. Aim 3 is focused on ensuring robust validation and quality control of new resources. An example is a validated antibody collection that can be used to assess differentiation, injury, and repair status of kidney cells and tissues. Aim 4 is to collaborate with the Consortium to prioritize, suspend, or implement new and improved imaging resources. Working in collaboration with the Consortium, KIDNIT will replace obsolete technologies by leveraging its ties with industry leaders, along with a strong track record of S10 funding, to offer the most recent developments in microscopy, specifically to advance the research of our investigator collective. Impact: KIDNIT will advance kidney research by providing Center and Consortium investigators with novel and bespoke imaging tools that will provide critical information about the location, dynamics, amounts, and function of kidney-associated molecules, organelles, cells, and tissues in normal and disease states.

摘要：绑架（肾脏成像：开发小说和创新工具）是匹兹堡的中心 肾脏研究的资源开发核心。与肾脏调查员和 Center的生理学和模型系统核心，绑架将建立和改编尖端的光线，超分辨率， 和电子显微镜成像方式和技术，以解决与肾脏有关的开放问题 功能和功能障碍。绑架的广泛而远程目标如下：目标1是为动态提供护理 用于成像工具开发的孵化器空间。绑架将利用该中心提供的资源 用于生物成像（CBI），这是该国最大的成像设施之一。 CBI有专家 教师，大量的研究技术人员和大量的成像设备，包括40多个 现代广阔的田野，共聚焦，两光子，TIRF，灯页，超分辨率和电子显微镜。 AIM 2是开发新的和定制的成像资源，工具和技术，以增强 并提高肾脏研究。绑架将利用CBI的教师专业知识来发展，适应和实施 肾脏研究人员的新成像工具。开发中的示例项目包括新颖的清理和3D 成像协议，以揭示和量化整个器官水平的肾脏结构和功能； 使用最近开发的变体分析正常和纤维化组织中的胶原蛋白组织和结构 极化光学显微镜（即时极化光学显微镜； ipol）；开发新协议 员工荧光寿命成像和荧光能量转移，以迅速评估肾细胞功能和 对细胞外提示的反应；使用高吞吐量平台来进行大规模筛查器官 形式和功能；和新型微流体室的发展。有机制可以确保培训 和信息传输。 AIM 3专注于确保对新资源的强大验证和质量控制。 一个例子是经过验证的抗体收集，可用于评估分化，伤害和维修状态 肾细胞和组织。目标4是与财团合作以优先，暂停或实施新的 并改善了成像资源。与财团合作，绑架将取代过时 通过利用与行业领导者的联系以及S10资金的良好记录来提供技术，以提供 显微镜检查的最新发展，特别是为了推进我们研究者集体的研究。 影响：绑架将通过为中心和财团调查员提供新颖和联盟的研究来推进肾脏研究 定制成像工具将提供有关位置，动态，数量和功能的关键信息 正常和疾病状态中与肾脏相关的分子，细胞器，细胞和组织。