UTHSC RBL Administrative Supplement For Antiviral Discovery & Development

UTHSC RBL 抗病毒药物发现行政补充

• 批准号: 10636200
• 负责人: Colleen B Jonsson
• 金额: $ 202.19万
• 依托单位: UNIVERSITY OF TENNESSEE HEALTH SCI CTR
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-15 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10636200
• 关键词: Administrative Supplement; Air; Air Movements; Animals; Area; Automation; Basic Science; Biological Assay; Bioluminescence; Biomedical Research; COVID-19; Cells; Chills; Clinical Research; Complement; Coupled; Data; Development; Disease; Equipment; Failure; Flow Cytometry; Fluorescence; Fostering; Functional disorder; Funding Opportunities; Future; Glycols; Goals; Hamsters; Health Sciences; Heating; Image; Immune response; Immunologics; Infection; Infectious Agent; Infrastructure; Laboratories; Lighting; Logic; Longevity; Maintenance; Manufacturer Name; Methods; Mission; Modernization; Monitor; Mus; Optics; Plants; Plaque Assay; Reaction Time; Recovery; Research; Research Personnel; Services; Structure; System; Tennessee; Therapeutic; Time; Translational Research; Tube; Universities; Update; Viral; Water; X-Ray Computed Tomography; animal imaging; biodefense; confocal imaging; design; emerging pathogen; exhaust; good laboratory practice; heat exchanger; high efficiency particulate air filter; imaging capabilities; imaging modality; imaging system; infectious disease treatment; operation; pathogen; pre-clinical; pressure; research and development; software systems; therapeutic development; trend; vaccine development; whole animal imaging

Project Summary The University of Tennessee Health Sciences Center (UTHSC) proposes to upgrade facilities and broaden research services in the existing UTHSC Regional Biocontainment Laboratory (RBL) located in Memphis, TN to support internal and external investigators in basic and translational research with emerging pathogens and select agents utilizing small animal. In Aim 1, upgrading the hardware and building automation system software that has been in place since 2009 is our top priority with this funding opportunity and will allow the UTHSC RBL to operate continuously well into the future. In Aim 2, we request support to expand existing infrastructure in order to use our current facility to its design capacity with additional caging and racks. Importantly, a major component of our plan is to upgrade our vivarium to house the Provantis Instem to provide support for preclinical services. In Aim 3 and 4, we request equipment for whole animal imaging and immunological analyses to strengthen our ability to meet the research service demands in terms of endpoints available to users; both internally and externally. In summary, this proposal seeks to: (1) upgrade and replace outdated or obsolete components of the building management system; (2) expand infrastructure for small animals other than mice, (3) upgrade current animal imaging capabilities and dedicate one animal biosafety level 3 (ABSL3) laboratory for the IVIS/CT imaging system, (4) enable preclinical services with a dedicate ABSL-3 area for Good Laboratory Practice (GLP) monitoring; and (5) equipment to enable immunological analyses currently not available (elispot) and replace an obsolete flow cytometry equipment with a spectral flow cytometer.

项目摘要 田纳西大学健康科学中心（UTHSC）提议升级设施并扩大 位于田纳西州孟菲斯的现有UTHSC区域生物疗养实验室（RBL）的研究服务 通过新兴病原体和外部研究人员在基础和转化研究中支持内部和外部研究人员 选择使用小动物的代理。在AIM 1中，升级硬件和构建自动化系统软件 自2009年以来，这是我们的重中之重，这是我们的重中之重，将允许UTHSC RBL可以在未来的情况下连续运行。在AIM 2中，我们请求支持以扩大现有基础架构 为了将我们当前的设施带到其设计能力，并带有其他笼子和机架。重要的是，专业 我们计划的组成部分是升级我们的Vivarium以容纳普罗凡尼式，以提供临床前的支持 服务。在AIM 3和4中，我们要求对全动物成像和免疫学分析的设备进行设备 增强我们根据用户可用的端点来满足研究服务需求的能力；两个都 内部和外部。总而言之，该提案寻求：（1）升级和更换过时或过时 建筑物管理系统的组成部分； （2）扩大小鼠以外的小动物的基础设施， （3）升级当前动物成像功能，并专用一个动物生物安全级别3（ABSL3）实验室 对于IVI/CT成像系统，（4）启用具有专用ABSL-3区域的临床前服务 练习（GLP）监测； （5）设备可实现当前不可用的免疫学分析（ELISPOT） 并用光谱流式细胞仪代替过时的流式细胞仪设备。