Tech Development Core

技术开发核心

• 批准号: 10242752
• 负责人: PARKER Bruce ANTIN
• 金额: $ 63.37万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-12 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10242752
• 关键词: Anatomy; Arizona; Biology; Birds; Chick; Chick Embryo; Chickens; Collaborations; Communities; Data; Data Analyses; Data Set; Databases; Development; Gene Expression; Genes; Genome; Genomics; Goals; Image; In Situ Hybridization; Laboratories; Laboratory Research; Link; Literature; Metadata; Molecular; Ontology; Publications; Publishing; Regulation; Research; Research Personnel; Resources; Voting; experimental analysis; high throughput screening; human disease; multiple data types; technology development; web site

The goal of the Technology Development Core is to expand the high quality in situ hybridization (ISH) expression dataset that is presently available through the GEISHA (gallus expression in situ hybridization analysis) database and user interface, and to integrate GEISHA data into AviPort. GEISHA is an established community resource for chicken embryo, providing ISH gene expression information and associated genomic metadata through in house ISH screens, literature curation and acquisition of expression data from other research laboratories. In recent years, the GEISHA project has generated a high quality dataset of 50,000 whole mount ISH images and associated metadata for approximately 3,200 developmentally regulated genes. With the focus of much biomedically related avian research on the molecular regulation of development and direct experimental analysis, high quality ISH information is of particularly high value to the avian research community. Though in past years GEISHA has been a stand-alone resource, going forward GEISHA will be integrated into AviPort High throughput ISH screens will continue to be conducted as this data has been voted the highest priority, after high quality genomes, for the avian research community. Additional ISH information will be curated from the literature and acquired from other researchers. In collaboration with the Curation Core, a chicken anatomical ontology will be created and used to annotate ISH chicken embryo images.

技术开发核心的目标是扩大高质量原位杂交 (ISH) 表达数据集，目前可通过 GEISHA（原鸡表达原位 杂交分析）数据库和用户界面，并将 GEISHA 数据集成到 AviPort 中。艺妓 是鸡胚胎的成熟社区资源，提供 ISH 基因表达 通过内部 ISH 筛选、文献整理和 从其他研究实验室获取表达数据。近年来，GEISHA 项目 生成了包含 50,000 个全安装 ISH 图像和相关元数据的高质量数据集 大约 3,200 个发育调控基因。重点关注许多与生物医学相关的鸟类 发育分子调控研究和直接实验分析、高质量原位杂交 信息对鸟类研究界具有特别高的价值。虽然在过去的几年里艺妓 一直是独立资源，未来 GEISHA 将集成到 AviPort 高吞吐量中 ISH 筛查将继续进行，因为该数据已被评为最高优先级，继高 为鸟类研究界提供优质基因组。其他 ISH 信息将由 文献并从其他研究人员那里获得。与 Curation Core 合作，进行了鸡解剖学研究 将创建本体并用于注释 ISH 鸡胚胎图像。