GEISHA: A Chicken Embryo Gene Expression Resource

GEISHA：鸡胚胎基因表达资源

• 批准号: 8519496
• 负责人: PARKER Bruce ANTIN
• 金额: $ 69.89万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-30 至 2015-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8519496
• 关键词: Adult; Amino Acid Sequence; Animal Model; Arizona; Biomedical Research; Birds; Chickens; Communities; Data; Data Analyses; Data Sources; Databases; Development; Embryo; Embryonic Development; Employee Strikes; Experimental Models; Funding; Gene Expression; Gene Expression Profiling; Genes; Genomics; Goals; Health; Housing; Human; Image; In Situ Hybridization; Java; Lead; Link; Literature; Metadata; Modeling; Mus; Online Mendelian Inheritance In Man; Ontology; Pattern; Peptide Sequence Determination; Publications; Publishing; RNA; Recording of previous events; Regulator Genes; Reporting; Research Personnel; Resources; Retrieval; Sequence Analysis; Services; Source; System; Xenopus; Zebrafish; base; data integration; gene function; human disease; improved; information display; mutant; repository; screening; tool; user-friendly

DESCRIPTION (Provided by Applicant): A central paradigm of biomedical research is that understanding gene function will ultimately lead to improvements in human health. A crucial first step is to know where and when genes are expressed during embryogenesis and in the adult. The chicken embryo provides many advantages for large-scale expression screening, including striking similarities to human, low expense, and a long history as an experimental model organism. The goal of this renewal application is to continue support and expansion of the GEISHA (gallus expression in situ hybridization analysis) gene expression database, an established community resource for chicken embryo in situ hybridization gene expression information and associated genomic metadata. Specific aims of this application are (1) to populate the GEISHA database with chicken gene expression information arising from targeted high throughput in situ hybridization screens, from the scientific literature, and from other researchers. High throughput in situ hybridization analyses will be performed to determine the expression patterns of developmental regulatory genes. In situ hybridization images and associated information will also be curated from publications and obtained from researchers. 2) The GEISHA database will be expanded to encompass new types of information and features. In situ hybridization images and associated data will be exchanged with other gene expression projects to enable cross-species display of expression information locally on each platform. The BioMart data integration system and a publication management system will also be added to enhance search and retrieval capabilities and to display gene related publications. 3) The user interface will be enhanced to accommodate new features and to facilitate navigation. Gene Expression Report pages will be reorganized and expanded to include detailed gene summary information, gene-related publications, and gene expression information for multiple species. User-friendly query features will be expanded to include searching by OMIM human disease gene and Gene Ontology terms. Through these enhancements, GEISHA will continue to provide a valuable and comprehensive resource for biomedical research. RELEVANCE: The objective of this project is to enhance an important existing gene expression resource for the chicken embryo, a recognized biomedical model of human disease. These services will provide valuable information that will assist efforts across a wide variety of related fields towards the identification of mechanisms underlying human diseases.

描述（由申请人提供）：生物医学研究的中心范式是，了解基因功能最终将导致人类健康的改善。 关键的第一步是知道在胚胎发生期间和成年人中表达基因的位置和何时。 鸡胚胎为大规模表达筛查提供了许多优势，包括与人类的相似之处，低费用以及作为实验模型生物的悠久历史。 这种更新应用的目的是继续支持和扩展地神沙群（Gallus表达原位杂交分析）基因表达数据库，这是一种既定的鸡胚胎的社区资源，用于原位杂交基因表达信息和相关的基因组元数据。 该应用程序的具体目的是（1）用鸡基因表达信息填充了地神沙数据库，该信息是由靶向高吞吐量的原位杂交筛选，科学文献和其他研究人员引起的。 将进行高通量的原位杂交分析，以确定发育调节基因的表达模式。 原位杂交图像和相关信息也将从出版物中策划并从研究人员那里获得。 2）将扩展地艺isa数据库，以涵盖新的信息和功能类型。 原位杂交图像和相关数据将与其他基因表达项目交换，以启用每个平台上本地表达信息的跨物种。 还将添加BiomArt数据集成系统和出版物管理系统，以增强搜索和检索功能并显示与基因相关的出版物。 3）将增强用户界面以适应新功能并促进导航。 基因表达报告页面将进行重新组织和扩展，以包括详细的基因摘要信息，与基因相关的出版物以及多种物种的基因表达信息。 用户友好的查询功能将扩展到包括OMIM人类疾病基因和基因本体学术语的搜索。 通过这些增强功能，艺妓将继续为生物医学研究提供宝贵而全面的资源。 相关性：该项目的目的是增强鸡胚胎的重要现有基因表达资源，鸡胚是一种公认​​的人类疾病生物医学模型。 这些服务将提供有价值的信息，这些信息将有助于各种相关领域的努力，以识别人类疾病潜在的机制。