Biological

生物

基本信息

批准号：
8335543
负责人：
MICHAEL V. SEIDEN
金额：
$ 5.38万
依托单位：
FOX CHASE CANCER CENTER
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-07-21 至 2014-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8335543
关键词：
Adopted Advisory Committees Anatomy Animals Basic Science Biological Cancer Center Support Grant Capital Financing Cathepsins Cells Clinical Research Comprehensive Cancer Center Consultations Data Doctor of Philosophy Elastases Electron Microscope Electron Microscopy Facility Equipment Evaluation Fees Fostering Fox Chase Cancer Center Foxes Funding Genomics Housing Image Imaging technology Instruction Investments Life Light Microscope Luciferases Magnetic Resonance Imaging Metalloproteases Microscope Molecular Optics Organ Organism Parents Peer Review Performance Policies Positioning Attribute Publications Recommendation Reporting Research Research Personnel Resolution Resource Sharing Resources Services Spectral Karyotyping Spectrum Analysis System Technical Expertise Technology Time Tissues Training Translational Research anticancer research assay development base cellular imaging cost gene function improved meetings new technology operation programs protocol development research study

项目摘要

PROJECT SUMMARY (See instructions): The Biological Imaging Facility (BIF) provides users with the equipment and technical expertise to conduct a wide array of imaging experiments that span from the ultrastructural level (Electron Microscope (EM)), cellular (Light Microscope (LM)) to tissues and organs in animals (Small Animal Imaging (SAI)). The BIF was created by merging previously separate approved CCSG Facilities for Cell Imaging, Electron Microscope (EM) and Small Animal Imaging services. The merger was based upon the recommendation of the Facility Parent Oversight Committee (FPOC) that was created since the last review to help prioritize shared resources. The FPOC noted that creating the BIF would consolidate overlapping scientific objectives of the three previous facilities and thus reduce operating costs by streamlining administrative and technical operations, without sacrificing any of the services that were offered by the original facilities. The services offered by this Facility have applications in clinical, translational and basic research. This is reflected by the center-wide use of the Facility resources. Since the last review, the BIF was utilized by 39 users from all five of the Center's Research Programs, 37 of whom were peer-review funded investigators. This Facility provides essential technical and scientific support as it has generated data that appeared in 60 peer reviewed publications, 18 of which reported collaborative studies that were fostered in part by interactions facilitated by the BIF. This Facility is a highly valued resource at the Center as is evident by the significant investment of Institutional funds for capital equipment. Equipment purchased during this funding period include a multispectral confocal microscope, Spectral Karyotype (SKY) equipment, a high-field MRI system (7-Tesla Bruker DRX) for anatomic imaging, a Caliperls IVIS spectrum that detects luciferase or fluorescently tagged cells in animals, and a Visen FMT2500 which detects the three dimensional distribution of enzymatically activated optical probes (metalloproteinases, cathepsin, elastase) or fluorescently tagged probes in live animals. These new technologies significantly expanded the capabilities of this Facility by providing users the ability to collect real-time information at the tissue, cellular and subcellular levels. The investment in equipment along with assay and protocol development conducted by a dedicated staff continue to provide users with the latest advances in imaging technologies. These vital services thus enhance the research programs of investigators and facilitate intra and interprogrammatic interactions.

项目摘要（参见说明）：生物成像设施 (BIF) 为用户提供设备和技术专业知识，以进行各种成像实验，范围从超微结构水平（电子显微镜 (EM)）、细胞（光学显微镜 (LM)）到组织和器官动物（小动物成像（SAI））。 BIF 是通过合并之前单独批准的 CCSG 设施而创建的，用于细胞成像、电子显微镜 (EM) 和小动物成像服务。此次合并是基于设施家长监督委员会 (FPOC) 的建议，该委员会自上次审查以来成立，旨在帮助优先考虑共享资源。 FPOC 指出，创建 BIF 将巩固之前三个设施的重叠科学目标，从而通过简化行政和技术来降低运营成本操作，而不牺牲原始设施提供的任何服务。该机构提供的服务可应用于临床、转化和基础研究。这反映在中心范围内对设施资源的利用。自上次审查以来，来自该中心所有五个研究项目的 39 名用户使用了 BIF，其中 37 名是同行评审资助的研究人员。该设施 BIF 提供了必要的技术和科学支持，因为它生成的数据出现在 60 份同行评审出版物中，其中 18 份出版物报告了合作研究，这些研究在一定程度上是通过 BIF 促进的互动而促进的。该设施是该中心的宝贵资源，机构资金对资本设备的大量投资就证明了这一点。在此资助期间购买的设备包括多光谱共焦显微镜、光谱核型 (SKY) 设备、用于解剖成像的高场 MRI 系统 (7-Tesla Bruker DRX)、用于检测荧光素酶或荧光标记细胞的 Caliperls IVIS 光谱。 Visen FMT2500 可检测活体动物中酶激活光学探针（金属蛋白酶、组织蛋白酶、弹性蛋白酶）或荧光标记探针的三维分布。这些新技术使用户能够收集组织、细胞和亚细胞水平的实时信息，从而显着扩展了该设施的功能。对设备的投资以及由专职人员进行的检测和方案开发继续为用户提供最新的成像技术的进步。因此，这些重要的服务增强了研究人员的研究计划，并促进计划内和计划间的互动。