University of Washington Center of Excellence in Opioid Addiction Research

华盛顿大学阿片类药物成瘾研究卓越中心

基本信息

批准号：
10611870
负责人：
Charles Chavkin
金额：
$ 132.76万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-07-01 至 2024-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10611870
关键词：
Address Affect Animals Anxiety Disorders Award Behavior Behavioral Brain Brain imaging CRISPR/Cas technology Calcium Signaling Canis familiaris Cells Collaborations Communities Complex Computer Analysis Confocal Microscopy Coupled DSM-V Development Disease Drug Addiction Drug usage Education and Outreach Educational workshop Endoscopy Ensure Fiber Fostering Funding Gene Expression Genetic Genetic Techniques Goals Image Imaging Device Infrastructure Institution Investigation Laboratories Light Link Mental Depression Methodology Modernization Molecular Molecular Genetics Monitor Morphine Mus National Institute of Drug Abuse Neurobiology Neurons Neurosciences Opiate Addiction Opioid Pain Participant Photometry Photons Pilot Projects Postdoctoral Fellow Productivity Qualifying Rattus Reagent Receptor Signaling Recording of previous events Reproducibility Research Research Personnel Resolution Resource Sharing Resources Risk Rodent Rodent Control Scientist Self Administration Substance Use Disorder Techniques Training Training and Education United States National Institutes of Health Universities Viral Viral Genes Visit Visualization Washington addiction archive data awake comorbidity computational neuroscience computerized tools data analysis pipeline design design and construction drug abstinence dysphoria genetic manipulation genetic resource graduate student imaging approach imaging genetics in vivo innovation insight meetings motivated behavior neural circuit neuroimaging novel novel therapeutics open data opioid use opioid use disorder optogenetics outreach recruit relapse risk research study sensor tool two photon microscopy two-photon undergraduate student vector web site

项目摘要

Overall Summary The “University of Washington Center of Excellence in Opioid Addiction Research” is designed to provide shared resources that would enhance efficiency and facilitate collaborative research for the study of the effects of opioids on neural circuits with the goal of understanding opioid addiction mechanisms and developing novel treatments for drug addiction. Center participants from 16 UW laboratories are using optogenetic control of rodent behavior, in vivo neuroimaging of single cell calcium signals and other receptor signaling probes, viral gene expression and CRISPR/cas9 manipulations to deconstruct and study opioid self-administration. This group has a long-history of highly productive, collaborative, cutting-edge research and training that will be strengthened by the shared resources provided by this award. The proposed Center would be comprised of four components: The Administrative Core will coordinate resource utilization, organize monthly Research Progress Meetings, organize training and outreach efforts. The Imaging and Neural Circuits Core would develop shared resources for in vivo brain imaging in rats and mice using 1-Photon endoscopy, Inscopix imaging, 2-Photon confocal microscopy, Spatial Light Modulation, fiber photometry and operant behaviors. The Molecular Genetics Resource Core would provide DIO-AAV / CRISPR-cas9 / Canine Adeno Viral reagents, develop new activity actuators and sensors and provide advanced training in cell-specific genetic manipulation coupled with behavioral and computational analysis. A Pilot Project Core would enable participants and trainees to initiate new projects utilizing the Imaging and Genetic Cores and would foster training and collaborations within the Center laboratory groups and university community at large. The Center would become a national neuroscience resource by providing training to visiting scientists, reagents for genetic manipulation and novel actuators/sensors on request from investigators at other institutions. It will provide workshops and summer courses for training advanced undergraduate and graduate students in optogenetics, computational neuroscience, and viral design & construction techniques. All of these components would be focused on understanding the changes in neural circuitry responsible for opioid addiction and on the development of new therapeutic tools based on these insights.

总体总结 “华盛顿大学阿片类药物成瘾研究卓越中心”旨在提供共享资源，这将提高效率并促进影响研究的合作研究阿片类药物对神经回路的影响，旨在了解阿片类药物成瘾机制并开发新的来自华盛顿大学 16 个实验室的中心参与者正在使用光遗传学控制来治疗药物成瘾。啮齿动物行为、单细胞钙信号和其他受体信号探针的体内神经成像、病毒基因表达和 CRISPR/cas9 操作来解构和研究阿片类药物的自我给药。该集团拥有高产、协作、前沿的研究和培训的悠久历史，这将是该奖项提供的共享资源将得到加强。拟议的中心将由以下人员组成。四个组成部分：行政核心将协调资源利用，组织每月研究进展会议、组织培训和外展工作。使用 1 光子内窥镜、Inscopix 开发大鼠和小鼠体内脑成像的共享资源成像、2 光子共焦显微镜、空间光调制、光纤光度测定和操作行为。分子遗传学资源核心将提供DIO-AAV / CRISPR-cas9 / 犬腺病毒试剂，开发新的活动执行器和传感器，并提供细胞特异性基因操作的高级培训加上行为和计算分析，试点项目核心将使参与者和学员利用成像和遗传核心启动新项目，并促进培训和中心实验室小组和整个大学社区之间的合作。通过为访问科学家、遗传试剂提供培训，成为国家神经科学资源它将根据其他机构的研究人员的要求提供操纵和新颖的执行器/传感器。用于培训光遗传学高级本科生和研究生的讲习班和暑期课程，所有这些组件都将是计算神经科学、病毒设计和构建技术。专注于了解导致阿片类药物成瘾的神经回路的变化以及基于这些见解开发新的治疗工具。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Recapitulating phenotypes of alcohol dependence via overexpression of Oprk1 in the ventral tegmental area of non-dependent TH::Cre rats.

通过在非依赖性 TH::Cre 大鼠腹侧被盖区过度表达 Oprk1 来概括酒精依赖性表型。

DOI：
10.1016/j.neuropharm.2023.109457
发表时间：
2023
期刊：
Neuropharmacology
影响因子：
4.7
作者：
Lepreux,Gaetan;Shinn,GraceE;Wei,Gengze;Suko,Azra;Concepcion,George;Sirohi,Sunil;SoonGo,Bok;Bruchas,MichaelR;Walker,BrendanM
通讯作者：
Walker,BrendanM

Kappa-opioid receptor activation reinstates nicotine self-administration in mice.

Kappa-阿片受体激活可恢复小鼠尼古丁的自我给药。

DOI：
10.1016/j.addicn.2022.100017
发表时间：
2022
期刊：
Addiction neuroscience
影响因子：
0
作者：
Gowrishankar,Raajaram;Gomez,Adrian;Waliki,Marie;Bruchas,MichaelR
通讯作者：
Bruchas,MichaelR

A unique role for cAMP signaling in microglia during opioid tolerance and withdrawal.

阿片类药物耐受和戒断期间小胶质细胞中 cAMP 信号传导的独特作用。

DOI：
10.1038/s41386-023-01685-2
发表时间：
2024
期刊：
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology
影响因子：
0
作者：
Coffey,KevinR;Neumaier,JohnF
通讯作者：
Neumaier,JohnF

Satb2 neurons in the parabrachial nucleus mediate taste perception.

DOI：
10.1038/s41467-020-20100-8
发表时间：
2021-01-11
期刊：
Nature communications
影响因子：
16.6
作者：
Jarvie BC;Chen JY;King HO;Palmiter RD
通讯作者：
Palmiter RD

A genetic variant of fatty acid amide hydrolase (FAAH) exacerbates hormone-mediated orexigenic feeding in mice.

DOI：
10.7554/elife.81919
发表时间：
2023-04-11
期刊：
eLife
影响因子：
7.7
作者：
Balsevich G;Petrie GN;Heinz DE;Singh A;Aukema RJ;Hunker AC;Vecchiarelli HA;Yau H;Sticht M;Thompson RJ;Lee FS;Zweifel LS;Chelikani PK;Gassen NC;Hill MN
通讯作者：
Hill MN