Human Embryonic Stem Cell Derived Cardiac Progenitors

人胚胎干细胞衍生的心脏祖细胞

基本信息

批准号：
7879383
负责人：
Paul Esteso
金额：
$ 4.76万
依托单位：
JOHNS HOPKINS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-12-01 至 2011-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7879383
关键词：
Accounting Activities of Daily Living Address Adult Age Anterior Appearance Area Arteries Birth Cardiac Cardiac Myocytes Cardiac Myosins Cardiovascular system Cause of Death Cell Communication Cell Count Cell Cycle Cell Transplantation Cell Transplants Cells Cellularity Cessation of life Characteristics Cicatrix Clinical Communication Congenital Heart Defects Congestive Heart Failure Connexin 43 DNA Developed Countries Down-Regulation Ectoderm Embryo Endocardium Endoderm Engraftment Experimental Models Family suidae Fellowship Frequencies Future Gene Expression Generations Genes Genetic Determinism Genetic Enhancer Element Genetic Markers Goals Green Fluorescent Proteins Heart Heart Diseases Heart Rate Heat shock proteins Human Human Characteristics Hypoxia Image Immune system Immunohistochemistry In Vitro Individual Infarction Injury Laboratories Lateral Left Length Ligation Literature Location Manuscripts Mechanics Mesoderm Modality Modeling Mus Myocardial Myocardial Degeneration Myocardial Infarction Myocardium Names National Research Service Awards Natural regeneration Necrosis Nuclear Patients Phase Phenotype Physiological Population Pregnancy Process Proliferating Quality of life Rattus Reporting Research Rodent Model Role Safety Signal Transduction Site Stem cells Stroke Structural Genes Techniques Text Therapeutic Therapeutic immunosuppression Time TimeLine Tissues Transgenic Organisms Transplantation Troponin T Undifferentiated Vertebrates base cardiogenesis embryonic stem cell genetic profiling hemodynamics human embryonic stem cell human embryonic stem cell line improved in vivo minimally invasive mouse model myocardin neural plate notochord older patient outcome forecast pericardial sac prevent progenitor promoter repaired safety testing somatic cell nuclear transfer statistics stem stem cell differentiation transcription factor ventricular hypertrophy

项目摘要

The hypoxic insult that occurs in myocardial infarctions damages the myocardium it nourishes, often leading to tissue necrosis. The necrotic area becomes an inelastic scar, often leading to ventricular hypertrophy and congestive heart failure. The purpose of my research under the NRSA fellowship is to study presidentially approved WAO1 human embryonic stem (hES) cells to define populations of cardiac progenitor cells (CPCs) suitable for transplantation into the site of myocardial injury. Thus, I hope to prevent myocardial degeneration and maintain functionality. The characteristics of mouse embryonic stem cell derived CPCs have been defined in my laboratory, and will serve as a basis to establish hES cell derived CPC populations. My first aim will be to establish the genetic markers characteristic of these cells in vivo and in vitro. My second aim is to determine gene expression levels to pinpoint CPCs temporal location during hES cell differentiation. My third aim is to establish a mechanism for selection of CPCs into pure populations. My fourth aim is to evaluate purified hES CPCs proliferative potential in vitro and in vivo. Future goals would include testing the safety and therapeutic potential of these cells in the pig myocardial infarction transplant model.

心肌梗塞中发生的缺氧损伤会损害其所滋养的心肌，通常会导致至组织坏死。坏死区域变成无弹性疤痕，常常导致心室肥大和充血性心力衰竭。我在 NRSA 奖学金下进行研究的目的是研究总统批准 WAO1 人胚胎干 (hES) 细胞定义心脏祖细胞 (CPC) 群体适合移植到心肌损伤部位。因此，我希望能够预防心肌退化并保持功能。小鼠胚胎干细胞衍生的 CPC 的特性在我的实验室中定义，并将作为建立 hES 细胞衍生的 CPC 群体的基础。我的第一个目的是在体内和体外建立这些细胞的遗传标记特征。我的第二个目标是确定基因表达水平以查明 hES 细胞分化过程中 CPC 的时间位置。我的第三个目标是建立CPC纯种群选拔机制。我的第四个目标是评估纯化的 hES CPC 的体外和体内增殖潜力。未来的目标将包括测试这些细胞在猪心肌梗塞移植模型中的安全性和治疗潜力。