Omics Core

组学核心

• 批准号: 10608169
• 负责人: Xiang Zhang
• 金额: $ 29.85万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-06-10 至 2026-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10608169
• 关键词: Address; Applications Grants; Attention; Award; Bioinformatics; Biological; Biological Assay; Centers of Research Excellence; Chemicals; Chromatography; Clinical Research; Communities; Complex Mixtures; Continuing Education; Data; Data Analyses; Data Collection; Data Reporting; Derivation procedure; Development; Dimensions; Disease; Drug Interactions; Education; Ensure; Environment; Equipment; Fee-for-Service Plans; Funding; Future; Goals; Health Sciences; Hepatology; Human Resources; Individual; Infrastructure; Institution; Intervention Studies; Leadership; Liver; Liver Dysfunction; Liver diseases; Malignant neoplasm of liver; Mass Spectrum Analysis; Methods; Modality; Modeling; Modification; Molecular Evolution; Peptides; Performance; Phase; Preparation; Privatization; Procedures; Protein Analysis; Protein Fragment; Proteins; Proteomics; Protocols documentation; RNA; Reagent; Research; Research Activity; Research Personnel; Research Support; Resources; S10 grant; Sampling; Science; Services; Standardization; Statistical Data Interpretation; Techniques; Technology; Toxicology; Training; Training and Education; Translational Research; United States National Institutes of Health; Universities; career; career development; clinical infrastructure; data sharing; design; epitranscriptomics; experimental study; fatty liver disease; innovation; instrument; liver development; liver injury; meetings; member; metabolomics; method development; molecular marker; non-alcoholic; novel; nutrition; operation; problem drinker; programs; sample collection; small molecule; success; theories; tool; toxicant

Abstract: The primary function of OMICS Core (Core B) is to provide members of the University of Louisville Hepatobiology and Toxicology COBRE with the expertise, resources, and facilities needed for metabolomic, proteomic, and bioinformatic analyses of biological samples collected to study liver injury, nutrition, gut:liver interactions, and liver:environment/toxicant/drug interactions. Core B personnel will assist with the design of sample collection protocols, will drive the development and the execution of novel and robust chromatographic methods for sample separation and mass spectrometric methods for the analysis of proteins, peptides, and metabolites with a goal of providing a greater understanding of the evolution of molecular mechanisms that correlate with or participate in liver dysfunction. Core B will ensure uniformity and standardization in sample preparation, method development, instrument performance, data collection, data reporting, and data sharing. Core B endeavors to contribute to the overall success and utility of the COBRE and to enhance the infrastructure and the technical and academic capabilities of the institution as a whole. In addition to its function as a service core, Core B will also promote the education and training of the COBRE members and other biomedical investigators with regards to the state-of-the-art omics technologies; with particular attention to the COBRE junior investigators. Furthermore, Core B will become an independent and self-supporting entity using a set comprehensive approaches that have been already implemented and demonstrated their success in our Phase I COBRE. During the Hepatobiology and Toxicology COBRE Phase II period the goals of Core B will be addressed by the following Specific Aims: 1) To support current COBRE and UofL Health Science Center (HSC) projects. 2) To educate and train mentees and other investigators. 3) To develop new protocols and techniques. 4) To become self-sustaining feature of the COBRE and UofL HSC.

摘要：Omics Core（核心B）的主要功能是为路易斯维尔大学的成员提供 肝脏生物学和毒理学鞋垫，具有代谢组学所需的专业知识，资源和设施， 收集的生物学样品的蛋白质组学和生物信息学分析，用于研究肝损伤，营养，肠道：肝脏 相互作用和肝脏：环境/有毒/药物相互作用。核心B人员将协助设计 样本收集协议将驱动新颖和稳健色谱的开发和执行 样品分离和质谱法的方法，用于分析蛋白质，肽和 代谢产物的目标是对分子机制的进化有更多的了解 与肝功能障碍相关或参与肝功能障碍。核心B将确保样本中的均匀性和标准化 准备，方法开发，仪器性能，数据收集，数据报告和数据共享。 核心B努力为毛绒的整体成功和实用性做出贡献，并增强基础设施 以及整个机构的技术和学术能力。除了它作为服务的功能 核心，核心B还将促进毛类成员和其他生物医学的教育和培训 关于最先进的Omics技术的研究人员；特别关注索贝尔少年 调查人员。此外，核心B将使用一组成为一个独立的自我支持实体 已经实施并证明了他们在我们阶段的成功的全面方法 我鹅 在肝生物学和毒理学cobre II期时期期间，核心B的目标将由 遵循特定目的： 1）支持当前的毛病和UOFL健康科学中心（HSC）项目。 2）教育和培训受训者和其他调查人员。 3）开发新的协议和技术。 4）成为山子和UOFL HSC的自我维持特征。