Implementation and Evaluation of Two SARS-CoV-2 Assays for Companion Animal Testing to Expand Vet-LIRN COVID-19 Capacity

用于伴侣动物测试的两种 SARS-CoV-2 检测的实施和评估，以扩大 Vet-LIRN COVID-19 能力

• 批准号: 10609646
• 负责人: Stephen Douglas Cole
• 金额: $ 5.09万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10609646
• 关键词: Implementation; Evaluation; Two; SARS; CoV

Project Summary Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a zoonotic disease that, in addition to its importance in the human pandemic, has caused spillover infection and onward spread in mink, white-tailed deer, and hamsters. New viral variants with different characteristics are constantly emerging, with different characteristics and in some cases, these variants are able to infect species of animals that were not originally susceptible. Therefore, ongoing surveillance through Vet-LIRN is essential for early recognition of spread within animals. Given that companion animals reside in 85 million US households, if SARS-CoV-2 were to gain the ability to spread amongst pets or between pets and humans, there could be immense public health consequence and consequence to wildlife populations. The objective of this proposal is to evaluate and validate novel testing methodologies including validating loop-mediated isothermal amplification (LAMP) in a variety of sample matrices in dogs and cats, and to perform rigorous evaluation of a commercially available ELISA kit. Our specific aims include: Aim 1: Assess the validity of a commercial colorimetric LAMP assay for the qualitative detection of SARS-CoV-2 RNA in 4 novel matrices collected from both dogs and cats. Aim 2: Validate a commercially available ELISA for SARS-CoV-2 antibodies in dogs and cats while building capacity to conduct SARS-CoV-2 serological testing. These aims will provide technician training, equipment, and expertise to provide capacity for SARS-CoV-2 testing in dogs and cats.

项目摘要 严重的急性呼吸综合征冠状病毒2（SARS-COV-2）是一种人畜共患病，在 除了其在人类大流行中的重要性外，还引起了溢出感和继续 散布在貂皮，白尾鹿和仓鼠中。具有不同特征的新病毒变体 不断出现，具有不同的特征，在某些情况下，这些变体能够 感染最初不易感的动物物种。因此，正在进行的监视 通过兽医lirn对于早期识别动物内传播至关重要。鉴于那个同伴 如果SARS-COV-2获得传播的能力，则动物居住在8500万美国家庭中 在宠物中或宠物和人类之间，可能会有巨大的公共卫生后果 以及对野生动植物种群的影响。该建议的目的是评估和验证 新型测试方法，包括验证循环介导的等温扩增（LAMP） 在狗和猫的各种样品矩阵中，并对 市售Elisa套件。我们的具体目的包括：目标1：评估a的有效性 商业比色灯测定于4个新颖的SARS-COV-2 RNA定性检测 从狗和猫那里收集的矩阵。 AIM 2：验证商用ELISA 狗和猫中的SARS-COV-2抗体，同时建立进行SARS-COV-2的能力 血清学测试。这些目标将为技术人员培训，设备和专业知识提供 为狗和猫提供SARS-COV-2测试的能力。