Automated Nucleic Acid Extraction and Purification System to Build Vet-LIRN Testing Capacity

自动化核酸提取和纯化系统，构建 Vet-LIRN 检测能力

• 批准号: 10820696
• 负责人: Stephen Douglas Cole
• 金额: $ 2.43万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10820696
• 关键词: Automated; Nucleic; Acid; Extraction; Purification

There is a public health need for veterinary laboratories to have significant capacity to test animal diagnostic specimens and food/feed samples rapidly and accurately for the presence of important pathogens such as Salmonella, Campylobacter, Listeria and SARS-CoV-2. The FDA Veterinary Laboratory Investigation and Response Network (Vet-LIRN) is a group of veterinary laboratories tasked with protecting the health of humans by being able to rapidly and robustly respond to outbreaks associated with animals and animal products. This equipment-only grant requests support for the purchase of a KingFisher Duo Prime semi-automated nucleic acid extraction system. This equipment will expand the capacity of a Vet-LIRN member laboratory (Ryan Small Animal Hospiital Clinical Microbiology Laboratory at the University of Pennsylvania’s) to perform additional molecular assays for case investigations and enhance their capability to handle additional specimens when surge capacity is potentially needed. It will also the laboratory to more fully engage with proficiency testing and interlaboratory comparison exercises developed by Vet-LIRN. Assays to be developed or implemented using thie equipment by the laboratory will include detection of Salmonella, Campylobacter, Listeria and imipenemase genes from carbapenem-resistant Enterobacterales.

公共卫生需要兽医实验室具有重大测试能力 动物诊断标本和食物/饲料样品迅速，准确地存在 重要的病原体，例如沙门氏菌，弯曲杆菌，李斯特菌和SARS-COV-2。 FDA 兽医实验室调查和反应网络（VET-LIRN）是一组兽医 实验室的任务是通过能够迅速，稳健地保护人类的健康 应对与动物和动物产品有关的暴发。此设备仅授予 要求支持购买翠鸟二重奏的半自动核酸 提取系统。该设备将扩大兽医LIRN成员实验室的能力 （瑞安小动物医院临床微生物学实验室 宾夕法尼亚州）进行额外的分子测定以进行案例调查并增强 当可能需要电涌能力时，它们可以处理其他规格的能力。会 还可以更充分地参与实验室的实验室和实验室间 VET-LIRN开发的比较练习。使用或实施的测定 实验室的Thie设备将包括沙门氏菌，弯曲杆菌，李斯特菌的检测 和抗碳青霉烯菌的丙咪成基因。