Animal Metabolism Phenotyping Core

动物代谢表型核心

基本信息

批准号：
10601026
负责人：
Stephen D Hursting
金额：
$ 14.71万
依托单位：
UNIV OF NORTH CAROLINA CHAPEL HILL
依托单位国家：
美国
项目类别：
财政年份：
1999
资助国家：
美国
起止时间：
1999-09-30 至 2026-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10601026
关键词：
Animals Appalachian Region Area Biological Assay Biological Models Biomedical Research Body Weight Calibration Calorimetry Cell model Cells Chills Collection Communities Computers and Advanced Instrumentation Data Analyses Dedications Development Diet Disease Documentation Energy Intake Ensure Exercise Experimental Designs Fatty acid glycerol esters Feces Food Preferences Funding Genetic Medicine Genetic Techniques Genetically Engineered Mouse Genomics Genus Hippocampus Goals Grant Guidelines Home Human IACUC Individual Industry Infrastructure Institution Intervention Maintenance Measurement Metabolism Methods Mitochondria Modeling Mus North Carolina Nutrient Nutritional Nutritional Study Obesity Obesity associated disease Phenotype Population Process Publications Quality Control Reproducibility Research Research Institute Research Personnel Resolution Resources Schedule Services Site Spirometry Study models Technology Therapeutic Thinness Time Training Universities Urine cell type clinically relevant disease phenotype endurance exercise energy balance experimental study feeding genetic manipulation industry partner instrumentation member metabolic phenotype metabolic rate metabolomics microbiome analysis mouse model next generation nutrition pre-clinical research programs response translational scientist

项目摘要

Project Summary – Animal Metabolism Phenotyping Core The Animal Metabolism Phenotyping Core is a high throughput facility dedicated to studying components of energy balance related to obesity and other nutritionally related diseases. The Core provides access to state- of-the-art methods, instrumentation, and access to mouse populations (such as Collaborative Cross lines and several genetically engineered mouse models unique to UNC) to assure a high level of quality control, reproducibility and maximal throughput that could not be achieved (or afforded) by individual investigators. The Core offers a variety of comprehensive, high-throughput services that can be interfaced with nutritional (and/or other environmental) treatments, such as long-term feeding of different diets or short-term provision of specific nutrients and therapeutic compounds. The Core provides investigators with critical information on the selection of appropriate diets, including the use of rigorous controls, and access to the Collaborative Cross and Diversity Outbred “next generation” mouse resource for nutrition and obesity research. The Core also provides training and professional development as it relates to studying components of murine energy balance. Based on strong demand from UNC NORC members, the Core implemented the scientific and technical support for mitochondria phenotyping of live cells and isolated mitochondria, an area of expertise for the Core Co- Directors. Core services were expanded from the University of North Carolina (UNC) Chapel Hill campus to include an additional site at the UNC Nutrition Research Institute (NRI) in Kannapolis, NC. The Core now has three mirrored facilities. Two of these facilities are located at the Chapel Hill campus in Kerr Hall and in the Genetic Medicine Building. The third facility is at the NRI. The NRI houses basic and translational scientists from UNC and other institutions/industry that rely on mouse models for studies consistent with the objectives of the UNC NORC. The purpose of this expansion was three-fold. First, it further enabled researchers housed at the NRI to utilize the UNC NORC core services. Second, it leveraged the extensive resources available at the NRI, including additional vivarium space. And third, it enables core services to be provided to a diverse group of external users, including academic partners from Appalachian State University, NC A&T University, NC Central University, NC State University, UNC Charlotte, UNC Greensboro, and industry partners.

项目摘要 – 动物代谢表型核心动物代谢表型核心是一个高通量设施，致力于研究动物代谢表型的组成部分与肥胖和其他营养相关疾病相关的能量平衡核心提供了获得状态的机会。最先进的方法、仪器和对小鼠群体的访问（例如协作交叉系和北卡罗来纳大学独有的几种基因工程小鼠模型），以确保高水平的质量控制，个别研究人员无法实现（或提供）的重现性和最大通量。 Core 提供各种全面的高通量服务，可以与营养（和/或其他环境）治疗，例如长期喂养不同的饮食或短期提供特定的核心为研究人员提供了有关选择的关键信息。适当的饮食，包括使用严格的控制，以及获得协作交叉和多样性用于营养和肥胖研究的远交“下一代”小鼠资源还提供培训。和专业发展，因为它涉及研究小鼠能量平衡的组成部分。应UNC NORC成员的需求，核心实施了科学和技术支持活细胞和分离线粒体的线粒体表型分析，这是核心合作伙伴的专业领域董事们。核心服务从北卡罗来纳大学 (UNC) 教堂山校区扩展至包括位于北卡罗来纳州坎纳波利斯的北卡罗来纳大学营养研究所 (NRI) 的另一个站点现在拥有三个站点。其中两个设施位于教堂山校区的 Kerr Hall 和 Genetic 中心。第三个设施位于 NRI，里面有来自 UNC 的基础科学家和转化科学家。以及其他依赖小鼠模型进行符合 UNC 目标的研究的机构/行业 NORC 的扩张有三个目的：首先，它进一步使 NRI 的研究人员能够其次，它利用了 NRI 的丰富资源。第三，它可以为不同的群体提供核心服务。外部用户，包括来自阿巴拉契亚州立大学、北卡罗来纳州农业与技术大学、北卡罗来纳州中部的学术合作伙伴大学、北卡罗来纳州立大学、北卡罗来纳大学夏洛特分校、北卡罗来纳大学格林斯伯勒分校以及行业合作伙伴。