Musculoskeletal Structure and Strength Core

肌肉骨骼结构和核心力量

基本信息

批准号：
10602565
负责人：
MATTHEW J SILVA
金额：
$ 14.35万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-04-15 至 2024-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10602565
关键词：
Address Adherence Animal Model Autopsy Biology Biomechanics Cartilage Communities Data Development Dual-Energy X-Ray Absorptiometry Ensure Equipment Evaluation Exhibits Fees Fostering Fracture Funding Future Goals Human Resources Image Image Enhancement Individual Investments Manuscripts Measures Mechanics Medicine Methods Microscope Morphology Muscle Muscle Tension Musculoskeletal Musculoskeletal structure Outcome Productivity Property Protocols documentation Publications Reporting Research Research Personnel Resolution Resources Roentgen Rays Schedule Services Structure Techniques Tendon structure Testing Tissues Training Training Programs Universities Washington animal tissue bone cellular imaging contrast enhanced cost effective functional outcomes imaging approach in vivo instrument long bone mechanical properties member microCT musculoskeletal imaging notch protein novel strategies online resource programs radiological imaging response skeletal disorder spine bone structure submicron

项目摘要

Summary – Resource Core B Measures of structure and strength are essential when assessing functional outcomes in animal models related to musculoskeletal biology and medicine. Evaluating these outcomes requires specialized equipment and expertise, which is impractical and inefficient to recreate in multiple labs. In response to this need, we established the Musculoskeletal Structure and Strength Core. This multifaceted resource supports our Research Community in understanding and implementing techniques to evaluate structure and mechanical properties of musculoskeletal tissues (bone, cartilage, disc, muscle, tendon) derived from animal models. We have had a strong impact. Since 2009, we have provided billable services to 94 unique investigators, who have reported structure or strength data in 176 manuscripts which have been cited 3266 times. The need for these services remains high, with 57 current and projected future users. In order to sustain and extend this resource going forward, we propose two Specific Aims. Aim 1 – Imaging: Maintain protocols and equipment, provide training and technical support, and perform imaging of musculoskeletal structures and tissues from animal models generated by Research Community investigators. Routine techniques include plane radiography, dual-energy X-ray absorptiometry (DXA), and micro-computed tomography (microCT), each available post mortem or in vivo. We have recently developed methods for contrast-enhanced imaging; we will make these available to our users and will continue to develop new approaches for imaging non-mineralized tissues (cartilage, disc, muscle, tendon). We will present regular seminars on imaging and strengthen our partnership with the Center for Cellular Imaging. Aim 2 – Mechanical Testing: Perform mechanical testing to assess the functional properties of musculoskeletal structures and tissues from animal models generated by Research Community investigators. Standard testing methods include long-bone bending, vertebral compression, tendon and tendon-to-bone tensile testing, and bone microindentation. Newly established methods include passive and active muscle tension testing and notched bending for fracture toughness. We will present seminars and provide training and enrichment to enhance understanding of biomechanics among Center investigators. In summary, Core B will provide critical support to members of our large Research Community to enable cost-effective utilization of methods to quantify morphology and mechanical properties from musculoskeletal tissues (bone, tendon, muscle, cartilage, disc). Through our efforts we will enhance research productivity and rigor, and provide access to state-of-the-art and emerging techniques.

摘要 – 资源核心 B 在评估动物模型的功能结果时，结构和强度的测量至关重要与肌肉骨骼生物学和医学相关的评估这些结果需要专门的知识。设备和专业知识，在多个实验室中重新创建是不切实际且低效的。需要，我们建立了肌肉骨骼结构和力量核心这个多方面的资源支持。我们的研究社区了解和实施技术来评估结构和肌肉骨骼组织（骨、软骨、椎间盘、肌肉、肌腱）的机械特性自 2009 年以来，我们已为 94 个独特的动物模型提供了收费服务。研究人员在 176 篇手稿中报告了结构或强度数据，这些手稿已被引用 3266 目前和预计的未来用户对这些服务的需求仍然很高。为了维持并扩展这一资源，我们提出了两个具体目标 1 – 成像：维持。协议和设备，提供培训和技术支持，并进行肌肉骨骼成像由研究团体研究人员生成的动物模型的结构和组织。技术包括平面放射线照相、双能 X 射线吸收测定法 (DXA) 和微计算断层扫描（microCT），每种都可用于尸检或体内检查。我们最近开发了方法。对比度增强成像；我们会将这些提供给我们的用户，并将继续开发新的我们将介绍常规的非矿化组织（软骨、椎间盘、肌肉、肌腱）成像方法。成像研讨会并加强我们与细胞成像中心的合作伙伴关系目标 2 –。机械测试：进行机械测试以评估肌肉骨骼的功能特性由研究团体标准研究人员生成的动物模型的结构和组织。测试方法包括长骨弯曲、椎体压缩、肌腱和肌腱-骨拉伸新建立的方法包括被动和主动肌肉张力。我们将举办研讨会并提供培训和断裂韧性测试和缺口弯曲。丰富内容以增强中心研究人员对生物力学的理解总之，核心 B。将为我们大型研究社区的成员提供关键支持，以实现具有成本效益的利用量化肌肉骨骼组织（骨、肌腱、通过我们的努力，我们将提高研究生产力和严谨性，并提供获得最先进的和新兴的技术。