Center for Membrane Proteins in Infectious Diseases (MPID)

传染病膜蛋白中心 (MPID)

基本信息

批准号：
8300877
负责人：
PETRA FROMME
金额：
$ 134.76万
依托单位：
ARIZONA STATE UNIVERSITY-TEMPE CAMPUS
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-09-30 至 2015-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8300877
关键词：
Affinity Base Sequence Biochemical Bioinformatics Biological Biology Cells Communicable Diseases Communities Complement Complementary DNA Complex Computer Simulation Coupled Crystallization Databases Detergents Development Dialysis procedure Drug Delivery Systems Escherichia coli Evaluation Fermentation Fluorescence Gene Expression Generations Genes Goals Health Host Defense Human In Vitro Informatics Insecta Knowledge Lipids Membrane Membrane Proteins Methodology Methods Micelles Modeling Molecular Mono-S Nucleic Acids Pathogenesis Pathway interactions Peptides Phase Plants Play Precipitation Prevention Procedures Process Protein Array Protein Structure Initiative Proteins Protocols documentation Quality Control Roentgen Rays Role Scheme Screening procedure Structure System Techniques Technology Vaccinia Vaccinium Validation Variant Viral Viral Matrix Proteins X-Ray Crystallography Yeasts base chemical property computerized tools design gene synthesis in vivo innovation magnetic beads multidisciplinary new technology novel pathogen protein expression protein purification protein structure protein structure prediction scale up technology development tool vector

项目摘要

The MPID Center for membrane protein structure determination is focused on an important biological and medically relevant theme. It aims to determine the structures of membrane proteins involved in pathogenesis, with a focus on viral membrane proteins, bacterial membrane proteins, and human membrane proteins involved in pathogenic pathways. Membrane proteins represent >60% of all drug targets and they are also key players in the pathogenesis of infectious diseases. A critical step for the elucidation of the complex processes that are catalyzed by membrane proteins is an understanding of the structure, dynamics and function of membrane proteins. Our knowledge of processes catalyzed by membrane proteins suffers mainly from the lack of information concerning their structure as less than 300 different membrane protein structures are known at present. The Center targets membrane proteins of important viral and bacterial pathogens, their infectious pathways, and molecules involved in host defense against the pathogens. This theme is unique and the results and structures determined by the Center will be highly relevant for human health worldwide. The structure determination of each of the targets may provide important clues for the understanding of the infectious disease pathways and can therefore form the basis for the treatment and prevention of infectious diseases. The major goal of the Center is to use the biological theme of membrane proteins in infectious diseases as the basis for the determination of more than 40 novel membrane protein structures and to develop new technology for high throughput membrane protein expression, isolation, functional characterization, crystallization, and structure determination. Therefore, the Center aims to significantly contribute to the main goal of the PSI structure initiative by solving structures of novel membrane proteins with a large sequence and structural coverage. The Center also aims to develop new bioinformatics tools for the analysis of potential targets, solved structures, and the prediction of membrane protein structures. The MPID center will also collaborate with the community and the PSI on the structure determination of target membrane proteins that complement the initial targets of the center.

MPID 膜蛋白结构测定中心专注于重要的生物学和医学相关主题。它旨在确定参与发病机制的膜蛋白的结构，重点关注参与致病途径的病毒膜蛋白、细菌膜蛋白和人类膜蛋白。膜蛋白占所有药物靶点的 60% 以上，也是传染病发病机制的关键参与者。阐明膜蛋白催化的复杂过程的关键一步是了解膜蛋白的结构、动力学和功能。我们对膜蛋白催化过程的了解主要是由于缺乏有关膜蛋白结构的信息，因为目前已知的不同膜蛋白结构不到 300 种。该中心的目标是重要的病毒和细菌病原体的膜蛋白、它们的感染途径以及参与宿主防御病原体的分子。这个主题是独特的，该中心确定的结果和结构将与全世界人类健康高度相关。每个靶点的结构确定可能为了解传染病途径提供重要线索，从而为传染病的治疗和预防奠定基础。该中心的主要目标是利用膜蛋白在传染病中的生物学主题作为确定40多种新型膜蛋白结构的基础，并开发高通量膜蛋白表达、分离、功能表征、结晶的新技术。，和结构测定。因此，该中心旨在通过解决具有大序列和结构覆盖的新型膜蛋白的结构，为PSI结构倡议的主要目标做出重大贡献。该中心还致力于开发新的生物信息学工具，用于分析潜在目标、解析结构和预测膜蛋白结构。 MPID 中心还将与社区和 PSI 合作确定靶膜蛋白的结构，以补充该中心的初始靶点。